Construct: ORF ccsbBroadEn_14758
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018893.1_s300c1, BRDN0000458189
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PIK3C3 (5289)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5289 | PIK3C3 | phosphatidylinositol 3-kina... | NM_002647.4 | 99.5% | 21.3% | (many diffs) |
| 2 | human | 5289 | PIK3C3 | phosphatidylinositol 3-kina... | NM_001308020.2 | 92.4% | 14.2% | (many diffs) |
| 3 | human | 5289 | PIK3C3 | phosphatidylinositol 3-kina... | XM_011526031.3 | 78.6% | 5.5% | (many diffs) |
| 4 | human | 5289 | PIK3C3 | phosphatidylinositol 3-kina... | XR_002958176.1 | 41.4% | (many diffs) | |
| 5 | human | 5289 | PIK3C3 | phosphatidylinositol 3-kina... | XR_935226.1 | 40% | (many diffs) | |
| 6 | human | 5289 | PIK3C3 | phosphatidylinositol 3-kina... | XR_001753204.2 | 39% | (many diffs) | |
| 7 | mouse | 225326 | Pik3c3 | phosphatidylinositol 3-kina... | NM_181414.5 | 87.4% | 21% | (many diffs) |
| 8 | mouse | 225326 | Pik3c3 | phosphatidylinositol 3-kina... | XR_385985.2 | 58.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 98
- ORF end:
- 680
- ORF length:
- 582
- Sequence:
-
1 AATGGTTTTA TTTTGACTGA TAGTGACCTG TTCGTTGCAA CAAATTGATG AGCAATGCTT 61 TTTTATAATG CCAACTTTGT ACAAAAAAGT TGGCACCATG GGGGAAGCAG AGAAGTTTCA 121 CTACATCTAT AGTTGTGACC TGGATATCAA CGTCCAGCTT AAGATAGGAA GCTTGGAAGG 181 GAAGAGAGAA CAAAAGAGTT ATAAAGCTGT CCTGGAAGAC CCAATGTTGA AGTTCTCAGG 241 ACTATATCAA GAGACATGCT CTGATCTTTA TGTTACTTGT CAAGTTTTTG CAGAAGGGAA 301 GCCTTTGGCC TTGCCAGTGA GAACATCCTA CAAAGCATTT AGTACAAGAT GGAACTGGAA 361 TGAATGGCTG AAACTACCAG TAAAATACCC TGACCTGCCC AGGAATGCCC AAGTGGCCCT 421 CACCATATGG GATGTGTATG GTCCCGGAAA AGCAGTGCCT GTAGGAGGAA CAACGGTTTC 481 GCTCTTTGGA AAATACGGCA TGTTTCGCCA AGGGATGCAT GACTTGAAAG TCTGGCCTAA 541 TGTAGAAGCA GATGGATCAG AACCCACAAA AACTCCTGGC AGAACAAGTA GCACTCTCTC 601 AGAAGATCAG ATGAGCCGTC TTGCCAAGCT CACCAAAGCT CATCGACAAG GACACATGGG 661 TGAAAGTAGA TTGGCTGGAT AGATTGACAT TTAGAGAAAT AGAAATGATA AATGAGAGTG 721 AAAAACGAAG TTCTAATTTC ATGTACCTGA TGGTTGAATT TCGATGTGTC AAGTGTGATG 781 ATAAGGAATA TGGTATTGTT TATTATGAAA AGGACGGTGA TGAATCATCT CCATTTTAAC 841 AAGTTTTGAA TTAGTGGAAA GTTCCTGACC CCCAGATGTC TTATGGAGAA TTTAGTTGAg 901 agcaaacacc acaagcttgc ccggagttta agaagtggac cttctgacca cgatctgaaa 961 cccaatgctg ccacgagaga tcagttaaat attattgtga gttatccacc aaccaagcaa 1021 cttacatatg aagaacaaga tcttgtttgg aagtttagat attatcttac gaatcaagaa 1081 aaagccttga caaaattctt gaaatgtgtt aattgggatc tacctcaaga ggccaaacag 1141 gccttggaac ttctgggaaa atggaagccg atggatgtag aggactcctt ggagctgtta 1201 tcctctcatt acaccaaccc aactgtgagg cgttatgctg ttgcccggtt gcgacaggcc 1261 gatgatgagg atttgttgat gtacctatta caattggtcc aggctctcaa atatgaaaat 1321 tttgatgata taaagaatgg attggaacct accaagaagg atagtcagag ttcagtgtca 1381 gaaaatgtgt caaattctgg aataaattct gcagaaatag atagctccca aattataacc 1441 agcccccttc cttcagtctc ttcacctcct cctgcatcaa aaacaaaaga agttccagat 1501 ggcgaaaatc tggaacaaga tctctgtacc ttcttgatat cgagagcctg caaaaactca 1561 acactggcta attatttata ctggtatgtg atagtggaat gtgaagatca agatactcag 1621 cagagagatc caaagaccca tgagatgtac ttgaacgtaa tgagaagatt cagccaagca 1681 ttgttgaagg gtgataagtc tgtcagagtt atgcgttctt tgctggctgc acaacagaca 1741 tttgtagatc ggttggtgca tctaatgaag gcagtacaac gcgaaagtgg aaatcgtaag 1801 aaaaagaatg agagactaca ggcattgctt ggagataatg aaaagatgaa tttgtcagat 1861 gtggaactta tcccgttgcc tttagaaccc caagtgaaaa ttagaggaat aattccggaa 1921 acagctacac tgtttaaaag tgcccttatg cctgCCCAGT GTTTTTTAAG ACGGGAGGAT 1981 GGAGGAAAAT ATCCCAGTTA TATTTAAGCA TGGAGATGAT TTACGTCAAG ATCAACTTAT 2041 TCTTCAAATC ATTTCACTCA TGGACAAGCT GTTACGGAAA GAAAATCTGG ACTGAAAATT 2101 GACACCTTAT AAGGTGTTAG CCACCAGTAC AAAACATGGC TTCATGCAGT TTATCCAGTC 2161 AGTTCCTGTG GCTGAAGTTC TTGATACAGA GGGAAGCATT CAGAACTTTT TTAGAAAATA 2221 TGCACCAAGT GAGAATGGGC CAAATGGGAT TAGTGCTGAG GTCATGGACA CTTACGTTAA 2281 AAGCTGTGCT GGATATTGCG TGATCACCTA TATACTTGGA GTTGGAGACA GGCACCTGGA 2341 TAACCTTTTG CTAACAAAAA CAGGCAAACT CTTCCACATA GACTTTGGAT ATATTTTGGG 2401 TCGGGATCCA AAGCCTCTTC CTCCACCAAT GAAGCTGAAT AAAGAAATGG TAGAAGGAAT 2461 GGGGGGCACA CAGAGTGAGC AGTACCAAGA GTTCCGTAAA CAGTGTTACA CGGCTTTCCT 2521 CCACCTGCGA AGGTATTCTA ATCTGATTTT GAACTTGTTT TCCTTGATGG TTGATGCAAA 2581 CATTCCAGAT ATTGCACTTG AACCAGATAA AACTGTGAAA AAGGTTCAGG ATAAATTCCG 2641 CTTAGACCTG TCGGATGAAG AGGCTGTGCA TTACATGCAG AGTCTGATTG ATGAGAGTGT 2701 CCATGCTCTT TTTGCTGCAG TGGTGGAACA GATTCACAAG TTTGCCCAGT ACTGGAGAAA 2761 ATTGCCAACT TTCTTGTACA AAGTTGGCAT TATAAGAAAG CATTGCTTAT CAATTTGTTG 2821 CAACGAACAG GTCACTATCA GTCAAAATAA AATCATTATT GCCATCCCAG CCTGA