Construct: ORF ccsbBroadEn_14760
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018978.1_s300c1, BRDN0000458274
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PIK3CB (5291)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5291 | PIK3CB | phosphatidylinositol-4,5-bi... | NM_006219.3 | 99.2% | 19.2% | (many diffs) |
| 2 | human | 5291 | PIK3CB | phosphatidylinositol-4,5-bi... | XM_006713659.3 | 99.2% | 19.2% | (many diffs) |
| 3 | human | 5291 | PIK3CB | phosphatidylinositol-4,5-bi... | XM_011512895.2 | 99.2% | 19.2% | (many diffs) |
| 4 | human | 5291 | PIK3CB | phosphatidylinositol-4,5-bi... | XM_017006619.1 | 99.2% | 19.2% | (many diffs) |
| 5 | human | 5291 | PIK3CB | phosphatidylinositol-4,5-bi... | XM_024453596.1 | 92.3% | 14.2% | (many diffs) |
| 6 | human | 5291 | PIK3CB | phosphatidylinositol-4,5-bi... | XR_002959542.1 | 63.9% | (many diffs) | |
| 7 | human | 5291 | PIK3CB | phosphatidylinositol-4,5-bi... | XM_024453597.1 | 62.7% | 6.2% | (many diffs) |
| 8 | human | 5291 | PIK3CB | phosphatidylinositol-4,5-bi... | XM_017006621.2 | 61.1% | 7.6% | (many diffs) |
| 9 | human | 5291 | PIK3CB | phosphatidylinositol-4,5-bi... | NM_001256045.1 | 54.1% | 6.8% | (many diffs) |
| 10 | mouse | 74769 | Pik3cb | phosphatidylinositol-4,5-bi... | NM_029094.3 | 87.3% | 17.6% | (many diffs) |
| 11 | mouse | 74769 | Pik3cb | phosphatidylinositol-4,5-bi... | XM_011242829.2 | 87.3% | 17.6% | (many diffs) |
| 12 | mouse | 74769 | Pik3cb | phosphatidylinositol-4,5-bi... | XM_011242830.2 | 87.3% | 17.6% | (many diffs) |
| 13 | mouse | 74769 | Pik3cb | phosphatidylinositol-4,5-bi... | XM_006511520.2 | 75% | 4.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 95
- ORF end:
- 746
- ORF length:
- 651
- Sequence:
-
1 CACGGTTTTA TTTTGACTGA TAGTGACCTG TTCGTTGCAA CAAATTGATG AGCAATGCTT 61 TTTTATAATG CCAACTTTGT ACAAAAAAGT TGGCATGTGC TTCAGTTTCC TAATGCCTCC 121 TGCTATGGCA GACATCCTTG ACATCTGGGC GGTGGATTCA CAGATAGCAT CTGATGGCTC 181 CATACCTGTG GATTTCCTTT TGCCCACTGG GATTTATATC CAGTTGGAGG TACCTCGGGA 241 AGCTACCATT TCTTATATTA AGCAGATGTT ATGGAAGCAA GTTCACAATT ACCCAATGTT 301 CAACCTCCTT ATGGATATTG ACTCCTATAT GTTTGCATGT GTGAATCAGA CTGCTGTATA 361 TGAGGAGCTT GAAGATGAAA CACGAAGACT CTGTGATGTC AGACCTTTTC TTCCAGTTCT 421 CAAATTAGTG ACAAGAAGTT GTGACCCAGG GGAAAAATTA GACTCAAAAA TTGGAGTCCT 481 TATAGGAAAA GGTCTGCATG AATTTGATTC CTTGAAGGAT CCTGAAGTAA ATGAATTTCG 541 AAGAAAAATG CGCAAATTCA GCGAGGAAAA AATCCTGTCA CTTGTGGGAT TGTCTTGGAT 601 GGACTGGCTA AAACAAACAT ATCCACCAGA GCATGAACCA TCCATCCCTG AAAACTTAGA 661 AGATAAACTT TATGGGGGAA AGCTCATCGT AGCTGTTCAT TTTGAAAACT GCCAGACGTG 721 TTTAGCTTTC AAGTGTCTCC TAATATGAAT CCTATCAAAG TAAATGAATT GGCAATCCAA 781 AAACGTTTGA CTATTCATGG GAAGGAGATG AGTTAGCCCC TATGATTATG TGTTGCAGTC 841 AGCGGAGAGT AGAATATGTT TTTGGTGATC ATCCACTATT CAGTTCCAGT ATATCCGGAA 901 CTGTGTGATG AACAGAAGcc tgccccattt tatacttgtg gaatgctgca agatcaagaa 961 aatgtatgaa caagaaatga ttgccataga ggctgccata aatcgaaatt catctaatct 1021 tcctcttcca ttaccaccaa agaaaacacg aattatttct catgtttggg aaaataacaa 1081 ccctttccaa attgtcttgg ttaagggaaa taaacttaac acagaggaaa ctgtaaaagt 1141 tcatgtcagg gctggtcttt ttcatggtac tgagctcctg tgtaaaacca tcgtaagctc 1201 agaggtatca gggaaaaatg atcatatttg gaatgaacca ctggaatttg atattaatat 1261 ttgtgactta ccaagaatgg ctcgattatg ttttgctgtt tatgcagttt tggataaagt 1321 aaaaacgaag aaatcaacga aaactattaa tccctctaaa tatcagacca tcaggaaagc 1381 tggaaaagtg cattatcctg tagcgtgggt aaatacgatg gtttttgact ttaaaggaca 1441 attgagaact ggagacataa tattacacag ctggtcttca tttcctgatg aactcgaaga 1501 aatgttgaat ccaatgggaa ctgttcaaac aaatccatat actgaaaatg caacagcttt 1561 gcatgttaaa tttccagaga ataaaaaaca accttattat taccctccct tcgataagat 1621 tattgaaaag gcagctgaga ttgcaagcag tgatagtgct aatgtgtcaa gtcgaggtgg 1681 aaaaaagttt cttcctgtat tgaaagaaat cttggacagg gatcccttgt ctcaactgtg 1741 tgaaaatgaa atggatctta tttggacttt gcgacaagac tgccgagaga ttttcccaca 1801 atcactgcca aaattactgc tgtcaatcaa gtggaataaa cttgaggatg ttgctcagct 1861 tcaggcgctg cttcagattt ggcctaaact gcccccccgg gaggccctag agcttctgga 1921 tttcaactat ccagaccagt acgttcgaga atatgctgta ggctgcctgc gacagatgag 1981 tgatgaagaa ctttctcaat atcttttaca actggtgcaa gtgttaaaat atgagccttt 2041 tcttgattgt gccctctcta gattcctatt agaaagagca cttggtaatc ggaggatagg 2101 gcagtttcta ttttggcatc ttaggtcaga agtgcacatt cctgctgtct cagtacaatt 2161 tggtgtcatc cttgaagcat actgccgggg aagtgtgggg cacatgaaag tgctttctaa 2221 gcaggttgaa gcactcaata agttaaaaac tttaaatagt ttaatcaaac tgaatgccgt 2281 gaagttaaac agagccaaag ggaaggaggc catgcatacc tgtttaaaac agagtgctta 2341 ccgggaagcc ctctctgacc tgcagtcacc cctgaaccca tgtgttatcc tctcagaact 2401 ctatgttgaa aagtgcaaat acatggattc caaaatgaag cctttgtggc tggtatacaa 2461 taacaaggta tttggtGAGG ATTCCAGTTG GGAGTGATTT TTAAAAAATG GTGATGATTT 2521 ACGACCAGAT AATGTTGACA CTCCCAAATG TTGCGCTGGA TGATTTTACT CTGGAAAGAA 2581 GCTGGTTGGA TTCTTCGGAT GTTGCCTTAT GGCTGTTAGC AAACAGGAGA TCGCTCTGGC 2641 NTCATTGAAG TTGTGAGCAC CTCTGAAACA ATTGCTGACA TTCAGCTGAA CAGTAGCAAT 2701 GTGGCTGCTG CAGCAGCCTT CAACAAAGAT GCCCTTCTGA ACTGGCTTAA AGAATACAAC 2761 TCTGGGGATG ACCTGGACCG AGCCATTGAG GAATTTACAC TGTCCTGTGC TGGCTACTGT 2821 GTAGCTTCTT ATGTCCTTGG GATTGGTGAC AGACATAGTG ACAACATCAT GGTCAAAAAA 2881 ACTGGCCAGC TCTTCCACAT TGACTTTGGA CATATTCTTG GAAATTTCAA ATCTAAGTTT 2941 GGCATTAAAA GGGAGCGAGT GCCTTTTATT CTTACCTATG ATTTCATCCA TGTCATTCAA 3001 CAAGGAAAAA CAGGAAATAC AGAAAAGTTT GGCCGGTTCC GCCAGTGTTG TGAGGATGCA 3061 TATCTGATTT TACGACGGCA TGGGAATCTC TTCATCACTC TCTTTGCGCT GATGTTGACT 3121 GCAGGGCTTC CTGAACTCAC ATCAGTCAAA GATATACAGT ATCTTAAGGA CTCTCTTGCA 3181 TTAGGGAAGA GTGAAGAAGA AGCACTCAAA CAGTTTAAGC AAAAATTTGA TGAGGCGCTC 3241 AGGGAAAGCT GGACTACTAA AGTGAACTGG ATGGCCCACA CAGTTCGGAA AGACTACAGA 3301 TCTTTGCCAA CTTTCTTGTA CAAAGTTGGC ATTATAAGAA AGCATTGCTT ATCAATTTGT 3361 TGCAACGAAC AGGTCACTAT CAGTCAAAAT AAAATCATTA TTTGCCATCC CCAG