Transcript: Mouse XM_011242829.2

PREDICTED: Mus musculus phosphatidylinositol 3-kinase, catalytic, beta polypeptide (Pik3cb), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Pik3cb (74769)
Length:
4715
CDS:
215..3409

Additional Resources:

NCBI RefSeq record:
XM_011242829.2
NBCI Gene record:
Pik3cb (74769)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_011242829.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000024790 GCTGTATATGAGGAACTGGAA pLKO.1 455 CDS 100% 2.640 2.112 N Pik3cb n/a
2 TRCN0000319776 GCTGTATATGAGGAACTGGAA pLKO_005 455 CDS 100% 2.640 2.112 N Pik3cb n/a
3 TRCN0000024789 GCACTTGATAATCGGAGGATT pLKO.1 2186 CDS 100% 4.950 3.465 N Pik3cb n/a
4 TRCN0000319718 GCACTTGATAATCGGAGGATT pLKO_005 2186 CDS 100% 4.950 3.465 N Pik3cb n/a
5 TRCN0000024793 GCCTCTTATGTCCTCGGCATT pLKO.1 2927 CDS 100% 4.050 2.835 N Pik3cb n/a
6 TRCN0000319778 GCCTCTTATGTCCTCGGCATT pLKO_005 2927 CDS 100% 4.050 2.835 N Pik3cb n/a
7 TRCN0000024791 GCACATTACGTTCCCAGAGAA pLKO.1 1669 CDS 100% 4.950 2.475 Y Pik3cb n/a
8 TRCN0000319777 GCACATTACGTTCCCAGAGAA pLKO_005 1669 CDS 100% 4.950 2.475 Y Pik3cb n/a
9 TRCN0000024792 CCTTTCCAAATTACCTTGGTT pLKO.1 1190 CDS 100% 3.000 1.500 Y Pik3cb n/a
10 TRCN0000039979 CCACTAATTCAGTTCCAGTAT pLKO.1 980 CDS 100% 4.950 3.465 N PIK3CB n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011242829.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01202 pDONR223 100% 87.9% 95.3% None (many diffs) n/a
2 ccsbBroad304_01202 pLX_304 4.2% 87.9% 95.3% V5 (many diffs) n/a
3 TRCN0000467602 TCTTCGCAGCGGTTACTCCATTGC pLX_317 14.5% 87.9% 95.3% V5 (many diffs) n/a
4 TRCN0000491617 TGTCAAAACTACAACGGAACACGC pLX_317 12.2% 87.9% 95.3% V5 (not translated due to prior stop codon) (many diffs) n/a
5 ccsbBroad304_14760 pLX_304 21.2% 87.4% 17.6% V5 (not translated due to prior stop codon) (many diffs) n/a
6 ccsbBroadEn_14760 pDONR223 51.7% 87.3% 17.6% None (many diffs) n/a
7 TRCN0000474394 GTGTCCGTATATGGCGTGCTCTTT pLX_317 13.2% 87.3% 17.6% V5 (not translated due to prior stop codon) (many diffs) n/a
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