Transcript: Human NM_001256045.1

Homo sapiens phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta (PIK3CB), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-09-10
Taxon:
Homo sapiens (human)
Gene:
PIK3CB (5291)
Length:
4797
CDS:
347..2095

Additional Resources:

NCBI RefSeq record:
NM_001256045.1
NBCI Gene record:
PIK3CB (5291)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001145643 AAAGAGCACTTGGTAATCGG pXPR_003 AGG 537 31% 4 0.6354 PIK3CB PIK3CB 76193
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001256045.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000010025 CGACAAGACTGCCGAGAGATT pLKO.1 566 CDS 100% 4.950 6.930 N PIK3CB n/a
2 TRCN0000194877 CCACATTGACTTTGGACATAT pLKO.1 1684 CDS 100% 13.200 9.240 N PIK3CB n/a
3 TRCN0000018340 GATTGTGCCCTCTCTAGATTC pLKO.1 839 CDS 100% 10.800 7.560 N PIK3CB n/a
4 TRCN0000196367 GCTACTGTGTAGCTTCTTATG pLKO.1 1602 CDS 100% 10.800 7.560 N PIK3CB n/a
5 TRCN0000009860 CATTCAGCTGAACAGTAGCAA pLKO.1 1468 CDS 100% 3.000 2.100 N PIK3CB n/a
6 TRCN0000010016 AGCACTTGGTAATCGGAGGAT pLKO.1 871 CDS 100% 2.640 1.848 N PIK3CB n/a
7 TRCN0000010017 CCCATGTGTTATCCTCTCAGA pLKO.1 1171 CDS 100% 2.640 1.848 N PIK3CB n/a
8 TRCN0000194772 CACATGAAAGTGCTTTCTAAG pLKO.1 995 CDS 100% 10.800 6.480 N PIK3CB n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001256045.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01202 pDONR223 100% 54.3% 54.3% None 0_1ins1413;117_118ins51 n/a
2 ccsbBroad304_01202 pLX_304 4.2% 54.3% 54.3% V5 0_1ins1413;117_118ins51 n/a
3 TRCN0000467602 TCTTCGCAGCGGTTACTCCATTGC pLX_317 14.5% 54.3% 54.3% V5 0_1ins1413;117_118ins51 n/a
4 TRCN0000491617 TGTCAAAACTACAACGGAACACGC pLX_317 12.2% 54.3% 54.3% V5 (not translated due to prior stop codon) 0_1ins1413;117_118ins51 n/a
5 ccsbBroad304_14760 pLX_304 21.2% 54.1% 6.8% V5 (not translated due to prior stop codon) (many diffs) n/a
6 ccsbBroadEn_14760 pDONR223 51.7% 54.1% 6.8% None (many diffs) n/a
7 TRCN0000474394 GTGTCCGTATATGGCGTGCTCTTT pLX_317 13.2% 54.1% 6.8% V5 (not translated due to prior stop codon) (many diffs) n/a
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