Construct: ORF ccsbBroadEn_14780
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018931.1_s300c1, BRDN0000458227
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRKACA (5566)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5566 | PRKACA | protein kinase cAMP-activat... | NM_002730.4 | 99.7% | 99.4% | 680T>N;682_683delCTinsNN |
| 2 | human | 5566 | PRKACA | protein kinase cAMP-activat... | NM_207518.3 | 96.5% | 95.4% | (many diffs) |
| 3 | human | 5568 | PRKACG | protein kinase cAMP-activat... | NM_002732.3 | 85.1% | 82.3% | (many diffs) |
| 4 | human | 5566 | PRKACA | protein kinase cAMP-activat... | XM_017026948.1 | 84% | 83.7% | 680T>N;682_683delCTinsNN;760_761ins165 |
| 5 | human | 5566 | PRKACA | protein kinase cAMP-activat... | NM_001304349.1 | 81.2% | 78.9% | (many diffs) |
| 6 | human | 5567 | PRKACB | protein kinase cAMP-activat... | XM_006710758.2 | 73.5% | 87.9% | (many diffs) |
| 7 | human | 5567 | PRKACB | protein kinase cAMP-activat... | NM_207578.3 | 55.3% | 67.5% | (many diffs) |
| 8 | human | 5567 | PRKACB | protein kinase cAMP-activat... | XM_017001713.2 | 55.3% | 67.5% | (many diffs) |
| 9 | mouse | 18747 | Prkaca | protein kinase, cAMP depend... | NM_008854.5 | 90.9% | 97.4% | (many diffs) |
| 10 | mouse | 18747 | Prkaca | protein kinase, cAMP depend... | NM_001277898.1 | 88.1% | 93.4% | (many diffs) |
| 11 | mouse | 18749 | Prkacb | protein kinase, cAMP depend... | NM_011100.4 | 78.1% | 91.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 98
- ORF end:
- 1151
- ORF length:
- 1053
- Sequence:
-
1 AAGGATTTTA TTTTGACTGA TAGTGACCTG TTCGTTGCAA CAAATTGATG AGCAATGCTT 61 TTTTATAATG CCAACTTTGT ACAAAAAAGT TGGCACCATG GGCAACGCCG CCGCCGCCAA 121 GAAGGGCAGC GAGCAGGAGA GCGTGAAAGA ATTCTTAGCC AAAGCCAAAG AAGATTTTCT 181 TAAAAAATGG GAAAGTCCCG CTCAGAACAC AGCCCACTTG GATCAGTTTG AACGAATCAA 241 GACCCTCGGC ACGGGCTCCT TCGGGCGGGT GATGCTGGTG AAACACAAGG AGACCGGGAA 301 CCACTATGCC ATGAAGATCC TCGACAAACA GAAGGTGGTG AAACTGAAAC AGATCGAACA 361 CACCCTGAAT GAAAAGCGCA TCCTGCAAGC TGTCAACTTT CCGTTCCTCG TCAAACTCGA 421 GTTCTCCTTC AAGGACAACT CAAACTTATA CATGGTCATG GAGTACGTGC CCGGCGGGGA 481 GATGTTCTCA CACCTACGGC GGATCGGAAG GTTCAGTGAG CCCCATGCCC GTTTCTACGC 541 GGCCCAGATC GTCCTGACCT TTGAGTATCT GCACTCGCTG GATCTCATCT ACAGGGACCT 601 GAAGCCGGAG AATCTGCTCA TTGACCAGCA GGGCTACATT CAGGTGACAG ACTTCGGTTT 661 CGCCAAGCGC GTGAAGGGCC GCACTTGGAC CTTGTGCGGC ACCCCTGAGT ACCTGGCCCC 721 TGAGATTATC CTGAGCAAAG GCTACAACAA GGCCGTGGAC TGGTGGGCCC TGGGGGNTNN 781 TATCTATGAA ATGGCCGCTG GCTACCCGCC CTTCTTCGCA GACCAGCCCA TCCAGATCTA 841 TGAGAAGATC GTCTCTGGGA AGGTGCGCTT CCCTTCCCAC TTCAGCTCTG ACTTGAAGGA 901 CCTGCTGCGG AACCTCCTGC AGGTAGATCT CACCAAGCGC TTTGGGAACC TCAAGAATGG 961 GGTCAACGAT ATCAAGAACC ACAAGTGGTT TGCCACAACT GACTGGATTG CCATCTACCA 1021 GAGGAAGGTG GAAGCTCCCT TCATACCAAA GTTTAAAGGC CCTGGGGATA CGAGTAACTT 1081 TGACGACTAT GAGGAAGAAG AAATCCGGGT CTCCATCAAT GAGAAGTGTG GCAAGGAGTT 1141 TTCTGAGTTT TTGCCAACTT TCTTGTACAA AGTTGGCATT ATAAGAAAGC ATTGCTTATC 1201 AATTTGTTGC AACGAACAGG TCACTATCAG TCAAAATAAA ATCATTATTG CCATCCAGCT 1261 GAACTTAC