Construct: ORF ccsbBroadEn_14788
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018986.1_s300c1, BRDN0000458282
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PRKCA (5578)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5578 | PRKCA | protein kinase C alpha | NM_002737.3 | 99.5% | 33% | (many diffs) |
| 2 | human | 5578 | PRKCA | protein kinase C alpha | XM_017024836.2 | 92.8% | 34.4% | (many diffs) |
| 3 | human | 5578 | PRKCA | protein kinase C alpha | XM_017024837.1 | 90.8% | 23.1% | (many diffs) |
| 4 | human | 5578 | PRKCA | protein kinase C alpha | XM_024450829.1 | 85.9% | 19% | (many diffs) |
| 5 | human | 5578 | PRKCA | protein kinase C alpha | XM_024450830.1 | 85.9% | 19% | (many diffs) |
| 6 | human | 5578 | PRKCA | protein kinase C alpha | XM_017024841.1 | 46.8% | 68.5% | (many diffs) |
| 7 | mouse | 18750 | Prkca | protein kinase C, alpha | NM_011101.3 | 90.6% | 32.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 106
- ORF end:
- 772
- ORF length:
- 666
- Sequence:
-
1 CCCAAATAAA TGATTTTATT TTGACTGATA GTGACCTGTT CGTTGCAACA AATTGATGAG 61 CAATGCTTTT TTATAATGCC AACTTTGTAC AAAAAAGTTG GCACCATGGC TGACGTTTTC 121 CCGGGCAACG ACTCCACGGC GTCTCAGGAC GTGGCCAACC GCTTCGCCCG CAAAGGGGCG 181 CTGAGGCAGA AGAACGTGCA CGAGGTGAAG GACCACAAAT TCATCGCGCG CTTCTTCAAG 241 CAGCCCACCT TCTGCAGCCA CTGCACCGAC TTCATCTGGG GGTTTGGGAA ACAAGGCTTC 301 CAGTGCCAAG TTTGCTGTTT TGTGGTCCAC AAGAGGTGCC ATGAATTTGT TACTTTTTCT 361 TGTCCGGGTG CGGATAAGGG ACCCGACACT GATGACCCCA GGAGCAAGCA CAAGTTCAAA 421 ATCCACACTT ACGGAAGCCC CACCTTCTGC GATCACTGTG GGTCACTGCT CTATGGACTT 481 ATCCATCAAG GGATGAAATG TGACACCTGC GATATGAACG TTCACAAGCA ATGCGTCATC 541 AATGTCCCCA GCCTCTGCGG AATGGATCAC ACTGAGAAGA GGGGGCGGAT TTACCTAAAG 601 GCTGAGGTTG CTGATGAAAA GCTCCATGTC ACAGTACGAG ATGCAAAAAA TCTAATCCCT 661 ATGGATCCAA ACGGGCTTTC AGATCCTTAT GTGAAGCTGA AACTTATTCC TGATCCCAAG 721 AATGAAAGCA AGCAAAAAAC CAAAACCATC CGCTCCACAC TAAATCCGCA GTGAAATGAA 781 TCCTTTACTT TCAATTTGAA ACCTTTCGAC AAAGACCGAC AACTGTCTGT AGAAATCTGG 841 GACTGGGAAC gaacaacaag gaatgacttc atgggatccc tttcctttgg agtttcggag 901 ctgatgaaga tgccggccag tggatggtac aagttgctta accaagaaga aggtgagtac 961 tacaacgtac ccattccgga aggggacgag gaaggaaaca tggaactcag gcagaaattc 1021 gagaaagcca aacttggccc tgctggcaac aaagtcatca gtccctctga agacaggaaa 1081 caaccttcca acaaccttga ccgagtgaaa ctcacggact tcaatttcct catggtgttg 1141 ggaaagggga gttttggaaa ggtgatgctt gccgacagga agggcacaga agaactgtat 1201 gcaatcaaaa tcctgaagaa ggatgtggtg attcaggatg atgacgtgga gtgcaccatg 1261 gtagaaaagc gagtcttggc cctgcttgac aaacccccgt tcttgacgca gctgcactcc 1321 tgcttccaga cagtggatcg gctgtacttc gtcatggaat atgtcaacgg tggggacctc 1381 atgtaccaca ttcagcaagt aggaaaattt AAGGAACCAC AAGCAGTATT CTATGCGGCA 1441 GAGATTTCCA TCGGATTGTC TTTCTTCATA AAAGAGGAAT CATTTATAGG GATCTGAAGT 1501 TAGATAACGT CATGTTGGAT TCAGAAGGAC ATATCAAAAT TGCTGACTTT GGGATGTGCA 1561 AGGAACACAT GATGGATGGA GTCACGACCA GGACCTTCTG TGGGACTCCA GATTATATCG 1621 CCCCAGAGAT AATCGCTTAT CAGCCGTATG GAAAATCTGT GGACTGGTGG GCCTATGGCG 1681 TCCTGTTGTA TGAAATGCTT GCCGGGCAGC CTCCATTTGA TGGTGAAGAT GAAGACGAGC 1741 TATTTCAGTC TATCATGGAG CACAACGTTT CCTATCCAAA ATCCTTGTCC AAGGAGGCTG 1801 TTTCTATCTG CAAAGGACTG ATGACCAAAC ACCCAGCCAA GCGGCTGGGC TGTGGGCCTG 1861 AGGGGGAGAG GGACGTGAGA GAGCATGCCT TCTTCCGGAG GATCGACTGG GAAAAACTGG 1921 AGAACAGGGA GATCCAGCCA CCATTCAAGC CCAAAGTGTG TGGCAAAGGA GCAGAGAACT 1981 TTGACAAGTT CTTCACACGA GGACAGCCCG TCTTAACACC ACCTGATCAG CTGGTTATTG 2041 CTAACATAGA CCAGTCTGAT TTTGAAGGGT TCTCGTATGT CAACCCCCAG TTTGTGCACC 2101 CCATCTTACA GAGTGCAGTA TTGCCAACTT TCTTGTACAA AGTTGGCATT ATAAGAAAGC 2161 ATTGCTTATC AATTTGTTGC AACGAACAGG TCACTATCAG TCAAAATAAA ATCATTATTT 2221 GCCATCCAGC TGATATCCCC TATAGTGAGT CGTATTACAT GGTCATAGCT GTTTTC