Construct: ORF ccsbBroadEn_14796
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000888.2_s300c1, BRDN0000458455
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRKCZ (5590)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5590 | PRKCZ | protein kinase C zeta | NM_002744.6 | 100% | 100% | |
| 2 | human | 5590 | PRKCZ | protein kinase C zeta | XM_017001790.1 | 98.6% | 98.6% | 974_997del |
| 3 | human | 5590 | PRKCZ | protein kinase C zeta | XM_011541773.1 | 91.2% | 86.1% | (many diffs) |
| 4 | human | 5590 | PRKCZ | protein kinase C zeta | XM_017001789.1 | 90.1% | 85% | (many diffs) |
| 5 | human | 5590 | PRKCZ | protein kinase C zeta | NM_001242874.2 | 82.2% | 81.4% | (many diffs) |
| 6 | human | 5590 | PRKCZ | protein kinase C zeta | XM_011541774.1 | 74.1% | 68.5% | (many diffs) |
| 7 | human | 5590 | PRKCZ | protein kinase C zeta | XR_002957131.1 | 73.8% | 1_157del;1732_1814del;2017_2405del | |
| 8 | human | 5590 | PRKCZ | protein kinase C zeta | XR_002957130.1 | 73.1% | (many diffs) | |
| 9 | human | 5590 | PRKCZ | protein kinase C zeta | NM_001033581.2 | 69% | 69% | 0_1ins549 |
| 10 | human | 5590 | PRKCZ | protein kinase C zeta | NM_001033582.2 | 69% | 69% | 0_1ins549 |
| 11 | human | 5590 | PRKCZ | protein kinase C zeta | XM_017001801.1 | 68.4% | 68.4% | 0_1ins561 |
| 12 | human | 5590 | PRKCZ | protein kinase C zeta | NM_001350803.1 | 68.1% | 68.1% | 0_1ins549;425_448del |
| 13 | human | 5590 | PRKCZ | protein kinase C zeta | NM_001350804.1 | 68.1% | 68.1% | 0_1ins549;425_448del |
| 14 | human | 5590 | PRKCZ | protein kinase C zeta | NR_146911.1 | 66.6% | (many diffs) | |
| 15 | human | 5590 | PRKCZ | protein kinase C zeta | XM_011541775.3 | 66.5% | 56.3% | (many diffs) |
| 16 | human | 5590 | PRKCZ | protein kinase C zeta | XM_011541776.1 | 61.5% | 56.8% | (many diffs) |
| 17 | human | 5590 | PRKCZ | protein kinase C zeta | XM_024448396.1 | 61.5% | 56.8% | (many diffs) |
| 18 | human | 5590 | PRKCZ | protein kinase C zeta | XM_017001800.1 | 60.9% | 56.2% | (many diffs) |
| 19 | human | 5590 | PRKCZ | protein kinase C zeta | XM_017001792.1 | 60.7% | 56.1% | (many diffs) |
| 20 | human | 5590 | PRKCZ | protein kinase C zeta | XM_017001793.1 | 60.7% | 56.1% | (many diffs) |
| 21 | human | 5590 | PRKCZ | protein kinase C zeta | XM_024448391.1 | 60.7% | 56.1% | (many diffs) |
| 22 | human | 5590 | PRKCZ | protein kinase C zeta | XM_017001797.1 | 60.1% | 55.5% | (many diffs) |
| 23 | human | 5590 | PRKCZ | protein kinase C zeta | NM_001350805.1 | 59.9% | 59.9% | 0_1ins711 |
| 24 | human | 5590 | PRKCZ | protein kinase C zeta | NM_001350806.1 | 59.9% | 59.9% | 0_1ins711 |
| 25 | human | 5590 | PRKCZ | protein kinase C zeta | XM_017001803.2 | 58.1% | 56.2% | (many diffs) |
| 26 | human | 5590 | PRKCZ | protein kinase C zeta | XR_001737315.2 | 57.6% | (many diffs) | |
| 27 | human | 5590 | PRKCZ | protein kinase C zeta | XM_017001802.1 | 52.8% | 48.2% | (many diffs) |
| 28 | human | 5590 | PRKCZ | protein kinase C zeta | XM_024448397.1 | 52.1% | 47.6% | (many diffs) |
| 29 | mouse | 18762 | Prkcz | protein kinase C, zeta | NM_008860.3 | 88.8% | 95.6% | (many diffs) |
| 30 | mouse | 18762 | Prkcz | protein kinase C, zeta | XM_011250207.2 | 78% | 84.6% | (many diffs) |
| 31 | mouse | 18762 | Prkcz | protein kinase C, zeta | XM_006538655.3 | 72.7% | 79.3% | (many diffs) |
| 32 | mouse | 18762 | Prkcz | protein kinase C, zeta | NM_001039079.2 | 61% | 67.7% | (many diffs) |
| 33 | mouse | 18762 | Prkcz | protein kinase C, zeta | XM_006538656.2 | 61% | 67.7% | (many diffs) |
| 34 | mouse | 18762 | Prkcz | protein kinase C, zeta | XM_017320042.1 | 61% | 67.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1842
- ORF length:
- 1776
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag 61 ttggcatgcc cagcaggacc ggccccaaga tggaagggag cggcggccgc gtccgcctca 121 aggcgcatta cgggggggac atcttcatca ccagcgtgga cgccgccacg accttcgagg 181 agctctgtga ggaagtgaga gacatgtgtc gtctgcacca gcagcacccg ctcaccctca 241 agtgggtgga cagcgaaggt gacccttgca cggtgtcctc ccagatggag ctggaagagg 301 ctttccgcct ggcccgtcag tgcagggatg aaggcctcat cattcatgtt ttcccgagca 361 cccctgagca gcctggcctg ccatgtccgg gagaagacaa atctatctac cgccggggag 421 ccagaagatg gaggaagctg taccgtgcca acggccacct cttccaagcc aagcgcttta 481 acaggagagc gtactgcggt cagtgcagcg agaggatatg gggcctcgcg aggcaaggct 541 acaggtgcat caactgcaaa ctgctggtcc ataagcgctg ccacggcctc gtcccgctga 601 cctgcaggaa gcatatggat tctgtcatgc cttcccaaga gcctccagta gacgacaaga 661 acgaggacgc cgaccttcct tccgaggaga cagatggaat tgcttacatt tcctcatccc 721 ggaagcatga cagcattaaa gacgactcgg aggaccttaa gccagttatc gatgggatgg 781 atggaatcaa aatctctcag gggcttgggc tgcaggactt tgacctaatc agagtcatcg 841 ggcgcgggag ctacgccaag gttctcctgg tgcggttgaa gaagaatgac caaatttacg 901 ccatgaaagt ggtgaagaaa gagctggtgc atgatgacga ggatattgac tgggtacaga 961 cagagaagca cgtgtttgag caggcatcca gcaacccctt cctggtcgga ttacactcct 1021 gcttccagac gacaagtcgg ttgttcctgg tcattgagta cgtcaacggc ggggacctga 1081 tgttccacat gcagaggcag aggaagctcc ctgaggagca cgccaggttc tacgcggccg 1141 agatctgcat cgccctcaac ttcctgcacg agagggggat catctacagg gacctgaagc 1201 tggacaacgt cctcctggat gcggacgggc acatcaagct cacagactac ggcatgtgca 1261 aggaaggcct gggccctggt gacacaacga gcactttctg cggaaccccg aattacatcg 1321 cccccgaaat cctgcgggga gaggagtacg ggttcagcgt ggactggtgg gcgctgggag 1381 tcctcatgtt tgagatgatg gccgggcgct ccccgttcga catcatcacc gacaacccgg 1441 acatgaacac agaggactac cttttccaag tgatcctgga gaagcccatc cggatccccc 1501 ggttcctgtc cgtcaaagcc tcccatgttt taaaaggatt tttaaataag gaccccaaag 1561 agaggctcgg ctgccggcca cagactggat tttctgacat caagtcccac gcgttcttcc 1621 gcagcataga ctgggacttg ctggagaaga agcaggcgct ccctccattc cagccacaga 1681 tcacagacga ctacggtctg gacaactttg acacacagtt caccagcgag cccgtgcagc 1741 tgaccccaga cgatgaggat gccataaaga ggatcgacca gtcagagttc gaaggctttg 1801 agtatatcaa cccattattg ctgtccaccg aggagtcggt gtgcccaact ttcttgtaca 1861 aagttggcat tataagaaag cattgcttat caatttgttg caacgaac