Transcript: Mouse XM_011250207.2

PREDICTED: Mus musculus protein kinase C, zeta (Prkcz), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Prkcz (18762)
Length:
4020
CDS:
139..1698

Additional Resources:

NCBI RefSeq record:
XM_011250207.2
NBCI Gene record:
Prkcz (18762)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_011250207.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000274628 CAGATGATGAGGACGTCATAA pLKO_005 1601 CDS 100% 13.200 18.480 N Prkcz n/a
2 TRCN0000022664 CGGTGATACAACAAGCACTTT pLKO.1 1131 CDS 100% 4.950 6.930 N Prkcz2 n/a
3 TRCN0000022665 CGACATCAAGTCTCATGCTTT pLKO.1 1449 CDS 100% 4.950 3.960 N Prkcz2 n/a
4 TRCN0000022873 GAAGTGCTCATCATTCATGTT pLKO.1 184 CDS 100% 4.950 3.960 N Prkcz n/a
5 TRCN0000274630 GAAGTGCTCATCATTCATGTT pLKO_005 184 CDS 100% 4.950 3.960 N Prkcz n/a
6 TRCN0000274588 ATCTCGGAAACATGATAATAT pLKO_005 570 CDS 100% 15.000 10.500 N Prkcz n/a
7 TRCN0000274629 TGTAATGCCCTGAGGAATAAA pLKO_005 2034 3UTR 100% 15.000 10.500 N Prkcz n/a
8 TRCN0000274627 CTTCCAAGCCAAGCGCTTTAA pLKO_005 315 CDS 100% 13.200 9.240 N Prkcz n/a
9 TRCN0000022871 GCACTGGGTGTCCTTATGTTT pLKO.1 1225 CDS 100% 5.625 3.938 N Prkcz n/a
10 TRCN0000022666 CGACCAGATTTACGCCATGAA pLKO.1 741 CDS 100% 4.950 3.465 N Prkcz2 n/a
11 TRCN0000022668 CCAGTAGATGACAAGAACGAT pLKO.1 499 CDS 100% 3.000 2.100 N Prkcz2 n/a
12 TRCN0000022869 CCAGTCCGAATTTGAAGGCTT pLKO.1 1632 CDS 100% 2.640 1.848 N Prkcz n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011250207.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000489417 TCCGCGACTATAACAATCCGTGAA pLX_317 19.5% 78% 84.4% V5 (many diffs) n/a
2 ccsbBroadEn_01284 pDONR223 100% 78% 84.6% None (many diffs) n/a
3 ccsbBroad304_01284 pLX_304 33.6% 78% 84.6% V5 (many diffs) n/a
4 TRCN0000468951 TCTAGCACTATCAGGCTTACCTCC pLX_317 19.9% 78% 84.6% V5 (many diffs) n/a
5 ccsbBroadEn_14796 pDONR223 0% 78% 84.6% None (many diffs) n/a
6 ccsbBroad304_14796 pLX_304 33.6% 78% 84.6% V5 (many diffs) n/a
7 TRCN0000471650 GCTGTAGTGTGCCCGCTGAGGCCT pLX_317 23.7% 78% 84.6% V5 (many diffs) n/a
8 TRCN0000488644 CACGCTAGCACGACATTCTTTGCC pLX_317 18.5% 78% 84.6% V5 (not translated due to prior stop codon) (many diffs) n/a
9 TRCN0000489003 GTAAATACAATCATGACTCATCGG pLX_317 20.2% 78% 84.6% V5 (not translated due to prior stop codon) (many diffs) n/a
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