Construct: ORF ccsbBroadEn_14798
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018870.1_s300c1, BRDN0000458166
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PRKG2 (5593)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5593 | PRKG2 | protein kinase cGMP-depende... | NM_001363401.2 | 99.3% | 31.2% | (many diffs) |
| 2 | human | 5593 | PRKG2 | protein kinase cGMP-depende... | NM_006259.2 | 99.3% | 31.2% | (many diffs) |
| 3 | human | 5593 | PRKG2 | protein kinase cGMP-depende... | NM_001282485.2 | 95.5% | 32.4% | (many diffs) |
| 4 | human | 5593 | PRKG2 | protein kinase cGMP-depende... | XM_017008413.1 | 95% | 30.7% | (many diffs) |
| 5 | human | 5593 | PRKG2 | protein kinase cGMP-depende... | XM_017008414.1 | 95% | 30.7% | (many diffs) |
| 6 | human | 5593 | PRKG2 | protein kinase cGMP-depende... | XM_017008415.1 | 95% | 30.7% | (many diffs) |
| 7 | human | 5593 | PRKG2 | protein kinase cGMP-depende... | XM_017008416.1 | 95% | 30.7% | (many diffs) |
| 8 | human | 5593 | PRKG2 | protein kinase cGMP-depende... | XM_017008417.1 | 91.3% | 31.9% | (many diffs) |
| 9 | human | 5593 | PRKG2 | protein kinase cGMP-depende... | XM_017008418.1 | 60.4% | 51.5% | (many diffs) |
| 10 | human | 5593 | PRKG2 | protein kinase cGMP-depende... | NM_001282480.1 | 44.4% | 9.9% | (many diffs) |
| 11 | human | 5593 | PRKG2 | protein kinase cGMP-depende... | NM_001282481.1 | 44.4% | 9.9% | (many diffs) |
| 12 | human | 5593 | PRKG2 | protein kinase cGMP-depende... | NM_001282482.1 | 40.6% | 6.6% | (many diffs) |
| 13 | human | 5593 | PRKG2 | protein kinase cGMP-depende... | NM_001282483.1 | 36.5% | 12.3% | (many diffs) |
| 14 | mouse | 19092 | Prkg2 | protein kinase, cGMP-depend... | NM_008926.4 | 88.2% | 29.5% | (many diffs) |
| 15 | mouse | 19092 | Prkg2 | protein kinase, cGMP-depend... | XM_006534820.3 | 52.9% | 48.7% | (many diffs) |
| 16 | mouse | 19092 | Prkg2 | protein kinase, cGMP-depend... | XM_006534821.2 | 33% | 12.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 98
- ORF end:
- 833
- ORF length:
- 735
- Sequence:
-
1 AATGATTTTA TTTTGACTGA TAGTGACCTG TTCGTTGCAA CAAATTGATG AGCAATGCTT 61 TTTTATAATG CCAACTTTGT ACAAAAAAGT TGGCACCATG GGAAATGGTT CAGTGAAACC 121 TAAACATTCT AAGCACCCAG ATGGACACTC TGGGAACCTC ACCACTGATG CTCTGCGGAA 181 CAAGGTGACA GAGCTGGAGA GAGAGTTGAG GAGGAAGGAT GCTGAGATCC AGGAGCGGGA 241 GTACCATTTG AAGGAGCTGC GGGAGCAGCT GTCGAAGCAG ACTGTGGCCA TTGCTGAACT 301 CACAGAGGAG CTCCAGAACA AGTGCATCCA GCTGAACAAG CTGCAGGATG TGGTGCATAT 361 GCAGGGAGGA AGCCCGCTTC AGGCCTCTCC AGATAAAGTG CCTCTTGAGG TCCACCGGAA 421 GACCTCTGGA TTGGTCTCTC TCCATAGCAG GAGGGGAGCA AAGGCTGGCG TGTCTGCTGA 481 GCCAACAACC CGGACCTATG ACCTGAACAA ACCCCCTGAA TTTTCCTTTG AGAAAGCAAG 541 AGTCAGAAAA GACTCCAGTG AGAAGAAGCT CATTACAGAT GCCCTTAATA AAAATCAGTT 601 TCTGAAAAGA CTGGATCCTC AGCAGATCAA AGACATGGTG GAATGCATGT ATGGGAGAAA 661 CTATCAGCAA GGGAGTTACA TTATTAAGCA AGGAGAACCA GGAAACCATA TCTTTGTGCT 721 GGCAGAGGGT CGACTAGAGG TGTTCCAAGG GGAGAAATTG CTGTCCTCCA TCCCTATGTG 781 GACCACATTT GGGGAGCTTG CCATTTTATA CACTGTACAA GGACTGCCTC TGTGAAAGCT 841 ATTACCAATG TTAAAACATG GGCACTAGAT CGAGAGGTAT TCCAGAATAT AATGGAGGAG 901 GGACAGTCca agctagagat gaacaataca gaaacttcct cagaagtgta tccttgctga 961 agaatttacc tgaagataaa ttaaccaaga tcattgactg cttggaagtg gaatactatg 1021 acaaaggaga ttacatcatt agagagggcg aggaaggaag tacctttttc attttggcaa 1081 aaggaaaggt aaaagtaaca cagagcacag aaggccatga tcaaccacag ctgataaaaa 1141 cactgcagaa aggagaatac tttggagaaa aagctcttat cagtgatgat gtcaggtcag 1201 ctaacattat tgctgaagaa aatgatgttg catgcctggt tatagatcga gaaacattca 1261 accaaactgt cggtacattt gaagagctgc aaaaatacct tgaaggatat gtggcaaacc 1321 tgaaccgtga tgatgaaaaa agacatgcga agcggtccat gtctaactgg aagctgtcca 1381 aagcactctc tctggaaatg attcagctga aggagaaggt ggccagattt tcctcatcat 1441 ccccattcca gaaccttgag attattgcaa cactgggcgt tggtgggttc ggaagagttg 1501 agcttgttaa agtaaaaaat gagaatgttg cttttgctat gaagtgtata aggaagaagc 1561 acatagttga caccaagcag CAGGAGCATG TCTACTCAGA GAAGAGATTC CTAGAGGAGC 1621 TGTGCTCTCC ATTTCATTGT GAATTTATAT CGTACTTTCA AGGACAATAA GTATGTATAC 1681 ATGCTTCTGG AGCNNTGCTA GGNTGGGGAG CTCTGGAGTA TATTAAGGGA CAGAGGCAGC 1741 TTTGATGAAC CCACCTCCAA ATTCTGCGTT GCTTGTGTGA CAGAAGCATT TGATTACCTG 1801 CATCGACTAG GTATTATCTA CAGAGACTTG AAACCAGAAA ACTTAATTCT AGATGCTGAG 1861 GGTTACCTTA AATTGGTTGA CTTTGGATTT GCGAAGAAAA TAGGGTCTGG ACAGAAAACA 1921 TGGACATTCT GTGGGACTCC AGAATATGTA GCTCCTGAAG TCATTCTCAA CAAGGGACAT 1981 GACTTCAGTG TGGATTTCTG GTCACTGGGA ATTCTAGTGT ATGAGCTCCT AACGGGCAAC 2041 CCACCCTTTT CTGGGGTTGA CCAAATGATG ACCTACAATT TGATTCTCAA AGGAATTGAA 2101 AAAATGGATT TTCCCAGGAA GATAACACGA CGACCTGAGG ATTTGATTCG GAGGCTTTGC 2161 AGGCAAAATC CAACAGAAAG GCTGGGAAAT CTGAAGAATG GAATAAATGA CATTAAGAAA 2221 CACAGGTGGT TAAATGGTTT TAATTGGGAG GGACTGAAAG CACGGAGCCT TCCATCACCT 2281 TTGCAAAGAG AGCTCAAGGG ACCCATAGAT CACAGCTACT TTGACAAATA TCCTCCTGAA 2341 AAGGGAATGC CTCCAGATGA GCTATCAGGC TGGGATAAAG ACTTCTTGCC AACTTTCTTG 2401 TACAAAGTTG GCATTATAAG AAAGCATTGC TTATCAATTT GTTGCAACGA ACAGGTCACT 2461 ATCAGTCAAA ATAAAATCAT TATTGCCATC CCAGCCTGAT AT