Construct: ORF ccsbBroadEn_14801
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018876.1_s300c1, BRDN0000458172
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- MAPK4 (5596)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5596 | MAPK4 | mitogen-activated protein k... | NM_001292040.2 | 80.3% | 4.6% | (many diffs) |
| 2 | human | 5596 | MAPK4 | mitogen-activated protein k... | NM_002747.4 | 43.3% | 1.8% | (many diffs) |
| 3 | human | 5596 | MAPK4 | mitogen-activated protein k... | XM_005258299.3 | 43.3% | 1.8% | (many diffs) |
| 4 | human | 5596 | MAPK4 | mitogen-activated protein k... | XM_011526074.2 | 43.3% | 1.8% | (many diffs) |
| 5 | human | 5596 | MAPK4 | mitogen-activated protein k... | XM_011526075.3 | 43.3% | 1.8% | (many diffs) |
| 6 | human | 5596 | MAPK4 | mitogen-activated protein k... | XM_011526076.2 | 43.3% | 1.8% | (many diffs) |
| 7 | human | 5596 | MAPK4 | mitogen-activated protein k... | XM_017025839.2 | 43.3% | 1.8% | (many diffs) |
| 8 | mouse | 225724 | Mapk4 | mitogen-activated protein k... | NM_172632.2 | 39.4% | 1.6% | (many diffs) |
| 9 | mouse | 225724 | Mapk4 | mitogen-activated protein k... | XM_006525900.3 | 39.4% | 1.6% | (many diffs) |
| 10 | mouse | 225724 | Mapk4 | mitogen-activated protein k... | XM_006525901.3 | 39.4% | 1.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 136
- ORF end:
- 244
- ORF length:
- 108
- Sequence:
-
1 AAGGATTTTA TTTTGACTGA TAGTGACCTG TTCGTTGCAA CAAATTGATG AGCAATGCTT 61 TTTTATAATG CCAACTTTGT ACAAAAAAGN TNGCCANCCA NGGNTGAGAA NGGTGGACTG 121 CATCGCCNNN GTCTTATGGG TATGACNNNG NTGGGCGCTT TGTTNACTTC NAACCCCTGG 181 GNNTCGGTGT CAATGGTTTG GTGCTGTCGG CCGTGGACAG CCGGGCCTGC CGGAAGGTCG 241 CTGTGAAGAA GATTGCCCTG AGCGATGCCC GCAGCATGAA GCACGCGCTC CGAGAGATCA 301 AGATCATTCG GCGCCTGGAC CACGACAACA TCGTCAAAGT GTACGAGGTG CTCGGTCCCA 361 AGGGCACTGA CCTGCAGGGT GAGCTGTTCA AGTTCAGCGT GGCGTACATC GTCCAGGAGT 421 ACATGGAGAC CGACCTGGCA CGCCTGCTGG AGCAGGGCAC GCTGGCAGAA GAGCATGCCA 481 AGCTGTTCAT GTACCAGCTG CTCCGCGGGC TCAAGTACAT CCACTCCGCC AACGTGCTGC 541 ACAGGGACCT GAAGCCCGCC AACATCTTCA TCAGCACAGA GGACCTCGTG CTCAAGATTG 601 GGGATTTCGG GTTGGCAAGG ATCGTTGATC AGCATTACTC CCACAAGGGT TATCTGTCAG 661 AAGGGTTGGT AACAAAGTGG TACCGTTCCC CGGCCGCCCG GCCCCGGTGG ACGGCGGCGC 721 CAGCCCCCAG TTCGACCTGG ACGTGTTCAT CTCCCGCGCC CTGAAGCTCT GCACCAAGCC 781 CGAGGACCTG CCGGACAATN AACTGGGCGA CCTCAATGGT GCGTGCATCC CCGAGCACCC 841 TGGCGACCTC GTGCAGACCG AGGCCTTCTC NNAAGAAAGG TGGTTGCCAA CTTTCTTGTA 901 CAAAGTTGGC ATTATANGAA AGCATTGCTT ATCAATTTGT GCAACGAACA GGTCACTATC 961 AGTCAAAATA AAATCATTAT TGCCATCCCA GCTGATATT