Transcript: Mouse NM_172632.2

Mus musculus mitogen-activated protein kinase 4 (Mapk4), mRNA.

Source:
NCBI, updated 2017-05-14
Taxon:
Mus musculus (mouse)
Gene:
Mapk4 (225724)
Length:
4660
CDS:
951..2702

Additional Resources:

NCBI RefSeq record:
NM_172632.2
NBCI Gene record:
Mapk4 (225724)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_172632.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000378394 AGGGCGAGCTCTTCAAGTTTA pLKO_005 1225 CDS 100% 13.200 18.480 N Mapk4 n/a
2 TRCN0000363392 GTTCATCTCGCGTGCCCTAAA pLKO_005 2561 CDS 100% 10.800 15.120 N Mapk4 n/a
3 TRCN0000023182 CACGCCAAGCTGTTCATGTAT pLKO.1 1323 CDS 100% 5.625 7.875 N Mapk4 n/a
4 TRCN0000023179 GCAAGGATCGTGGATCAACAT pLKO.1 1464 CDS 100% 4.950 6.930 N Mapk4 n/a
5 TRCN0000023181 CGACAACATTGTCAAGGTGTA pLKO.1 1172 CDS 100% 4.050 3.240 N Mapk4 n/a
6 TRCN0000362008 GTGACCCACTTAGTCTCAAAT pLKO_005 3052 3UTR 100% 13.200 9.240 N Mapk4 n/a
7 TRCN0000362007 ATTACTCACACAAGGGTTATC pLKO_005 1483 CDS 100% 10.800 7.560 N Mapk4 n/a
8 TRCN0000023183 GCATCCTTACATGAGCCCGTA pLKO.1 1874 CDS 100% 2.160 1.512 N Mapk4 n/a
9 TRCN0000023180 CTGGAGAAGATCCTGACCTTT pLKO.1 1815 CDS 100% 4.950 2.970 N Mapk4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_172632.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01286 pDONR223 100% 87.1% 93.5% None (many diffs) n/a
2 ccsbBroad304_01286 pLX_304 0% 87.1% 93.5% V5 (many diffs) n/a
3 TRCN0000472205 CCCGGTACAACTATCCGTGCCAGA pLX_317 22.5% 87.1% 93.5% V5 (many diffs) n/a
4 TRCN0000488020 GCGATGGCTAGATTTTCGAGTCCA pLX_317 16.9% 82.5% 88.4% V5 (not translated due to frame shift) (many diffs) n/a
5 TRCN0000489673 GATACCCGTTCCACCGACTGCGGC pLX_317 7.7% 82.4% 79.8% V5 (not translated due to prior stop codon) (many diffs) n/a
6 ccsbBroadEn_14801 pDONR223 100% 39.4% 1.6% None (many diffs) n/a
7 ccsbBroad304_14801 pLX_304 0% 39.4% 1.6% V5 (not translated due to prior stop codon) (many diffs) n/a
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