Construct: ORF ccsbBroadEn_14808

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003307.2_s300c1, BRDN0000458287
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MAP2K2 (5605)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5605 MAP2K2 mitogen-activated protein k... NM_030662.3 99.8% 100% 453C>T;660C>A
2 human 5605 MAP2K2 mitogen-activated protein k... XM_006722799.2 76.5% 76.7% 453C>T;660C>A;705_706ins279
3 human 5605 MAP2K2 mitogen-activated protein k... XM_017026989.1 75.2% 67.6% (many diffs)
4 human 5605 MAP2K2 mitogen-activated protein k... XM_017026990.1 70.7% 50% (many diffs)
5 human 407835 LOC407835 mitogen-activated protein k... NR_002144.1 65.9% (many diffs)
6 human 5605 MAP2K2 mitogen-activated protein k... XM_017026991.1 61.5% 60.2% (many diffs)
7 mouse 26396 Map2k2 mitogen-activated protein k... NM_001347144.1 87.2% 94.2% (many diffs)
8 mouse 26396 Map2k2 mitogen-activated protein k... NM_023138.5 87% 94% (many diffs)
9 mouse 26396 Map2k2 mitogen-activated protein k... XM_006513684.3 45.1% 48.2% (many diffs)
10 mouse 26396 Map2k2 mitogen-activated protein k... XM_006513683.3 45% 48.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1266
ORF length:
1200
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag
61ttggcatgct ggcccggagg aagccggtgc tgccggcgct caccatcaac cctaccatcg
121ccgagggccc atcccctacc agcgagggcg cctccgaggc aaacctggtg gacctgcaga
181agaagctgga ggagctggaa cttgacgagc agcagaagaa gcggctggaa gcctttctca
241cccagaaagc caaggtcggc gaactcaaag acgatgactt cgaaaggatc tcagagctgg
301gcgcgggcaa cggcggggtg gtcaccaaag tccagcacag accctcgggc ctcatcatgg
361ccaggaagct gatccacctt gagatcaagc cggccatccg gaaccagatc atccgcgagc
421tgcaggtcct gcacgaatgc aactcgccgt acatcgtggg cttctacggg gccttctaca
481gtgacgggga gatcagcatt tgcatggaac acatggatgg cggctccctg gaccaggtgc
541tgaaagaggc caagaggatt cccgaggaga tcctggggaa agtcagcatc gcggttctcc
601ggggcttggc gtacctccga gagaagcacc agatcatgca ccgagatgtg aagccctcca
661acatcctcgt gaactctaga ggggagatca agctgtgtga cttcggggtg agcggccagc
721tcatagactc catggccaac tccttcgtgg gcacgcgctc ctacatggct ccggagcggt
781tgcagggcac acattactcg gtgcagtcgg acatctggag catgggcctg tccctggtgg
841agctggccgt cggaaggtac cccatccccc cgcccgacgc caaagagctg gaggccatct
901ttggccggcc cgtggtcgac ggggaagaag gagagcctca cagcatctcg cctcggccga
961ggccccccgg gcgccccgtc agcggtcacg ggatggatag ccggcctgcc atggccatct
1021ttgaactcct ggactatatt gtgaacgagc cacctcctaa gctgcccaac ggtgtgttca
1081cccccgactt ccaggagttt gtcaataaat gcctcatcaa gaacccagcg gagcgggcgg
1141acctgaagat gctcacaaac cacaccttca tcaagcggtc cgaggtggaa gaagtggatt
1201ttgccggctg gttgtgtaaa accctgcggc tgaaccagcc cggcacaccc acgcgcaccg
1261ccgtgtgccc aactttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt
1321gttgcaacga ac

Download FASTA (ORF) (Full)