Transcript: Human NM_030662.3

Homo sapiens mitogen-activated protein kinase kinase 2 (MAP2K2), mRNA.

Source:
NCBI, updated 2019-08-03
Taxon:
Homo sapiens (human)
Gene:
MAP2K2 (5605)
Length:
1759
CDS:
255..1457

Additional Resources:

NCBI RefSeq record:
NM_030662.3
NBCI Gene record:
MAP2K2 (5605)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001147805 AAGCACCAGATCATGCACCG pXPR_003 AGG 575 48% 5 0.5921 MAP2K2 MAP2K2 77600
2 BRDN0001147931 GGATTCCCGAGGAGATCCTG pXPR_003 GGG 507 42% 4 0.4451 MAP2K2 MAP2K2 77603
3 BRDN0001146932 ACGGCGAGTTGCATTCGTGC pXPR_003 AGG 368 31% 3 0.2132 MAP2K2 MAP2K2 77602
4 BRDN0001147718 GGCCCATCCCCTACCAGCGA pXPR_003 GGG 77 6% 1 0.0533 MAP2K2 MAP2K2 77601
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_030662.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000199043 CGACAGCGCATGCAGGAACGG pLKO.1 1593 3UTR 100% 0.000 0.000 N MAP2K2 n/a
2 TRCN0000195448 CGAGGTGGAAGAAGTGGATTT pLKO.1 1370 CDS 100% 10.800 7.560 N MAP2K2 n/a
3 TRCN0000199414 CGAATGCAACTCGCCGTACAT pLKO.1 623 CDS 100% 4.950 3.465 N MAP2K2 n/a
4 TRCN0000342310 CGAATGCAACTCGCCGTACAT pLKO_005 623 CDS 100% 4.950 3.465 N MAP2K2 n/a
5 TRCN0000196991 GCATTTGCATGGAACACATGG pLKO.1 685 CDS 100% 4.050 2.835 N MAP2K2 n/a
6 TRCN0000007008 CTGGACTATATTGTGAACGAG pLKO.1 1218 CDS 100% 2.640 1.584 N MAP2K2 n/a
7 TRCN0000007007 TGGACTATATTGTGAACGAGC pLKO.1 1219 CDS 100% 2.160 1.296 N MAP2K2 n/a
8 TRCN0000007006 GACTATATTGTGAACGAGCCA pLKO.1 1221 CDS 100% 0.660 0.396 N MAP2K2 n/a
9 TRCN0000195037 CTTCCAGGAGTTTGTCAATAA pLKO.1 1277 CDS 100% 13.200 6.600 Y MAP2K2 n/a
10 TRCN0000342312 CTTCCAGGAGTTTGTCAATAA pLKO_005 1277 CDS 100% 13.200 6.600 Y MAP2K2 n/a
11 TRCN0000007005 CCAACATCCTCGTGAACTCTA pLKO.1 847 CDS 100% 4.950 2.475 Y MAP2K2 n/a
12 TRCN0000195427 CGAACTCAAAGACGATGACTT pLKO.1 449 CDS 100% 4.950 2.475 Y MAP2K2 n/a
13 TRCN0000342309 CGAACTCAAAGACGATGACTT pLKO_005 449 CDS 100% 4.950 2.475 Y MAP2K2 n/a
14 TRCN0000011062 CAACATCCTCGTGAACTCTAG pLKO.1 848 CDS 100% 4.050 2.025 Y MAP2K2 n/a
15 TRCN0000342311 CAACATCCTCGTGAACTCTAG pLKO_005 848 CDS 100% 4.050 2.025 Y MAP2K2 n/a
16 TRCN0000055065 CCTCCGAGAGAAGCACCAGAT pLKO.1 803 CDS 100% 1.350 0.675 Y Map2k2 n/a
17 TRCN0000288078 CCTCCGAGAGAAGCACCAGAT pLKO_005 803 CDS 100% 1.350 0.675 Y Map2k2 n/a
18 TRCN0000199022 CCCTTCCAGCTGAGGACAGGC pLKO.1 1518 3UTR 100% 0.000 0.000 Y MAP2K2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_030662.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000488054 AAGGTTTGCCGATAAATCTTAGCG pLX_317 23.1% 100% 100% V5 (not translated due to prior stop codon) n/a
2 TRCN0000487985 AGCCCTCCCGCTAGCATAACCTAG pLX_317 26.4% 99.9% 99.7% V5 1200_1201insG n/a
3 ccsbBroadEn_14808 pDONR223 0% 99.8% 100% None 453C>T;660C>A n/a
4 ccsbBroad304_14808 pLX_304 0% 99.8% 100% V5 453C>T;660C>A n/a
5 TRCN0000465915 AACCGTCGGGCCCACCATGAAATT pLX_317 17.9% 99.8% 100% V5 453C>T;660C>A n/a
6 TRCN0000465689 TGACCGAAAAAAAATTTTTCGTTT pLX_317 17.9% 99.5% 68.1% V5 (not translated due to prior stop codon) (many diffs) n/a
7 ccsbBroadEn_10634 pDONR223 100% 90.9% 88% None (many diffs) n/a
8 ccsbBroad304_10634 pLX_304 0% 90.9% 88% V5 (many diffs) n/a
9 TRCN0000477744 AAGCGGTCACCAGAATGAGGGTTC pLX_317 27.6% 90.9% 88% V5 (many diffs) n/a
10 TRCN0000487698 GCGCTTTTCCTGCACCTTGACTTA pLX_317 23.3% 76.6% 76.7% V5 (not translated due to prior stop codon) 660C>A;706_984del n/a
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