Construct: ORF ccsbBroadEn_14831
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018965.1_s300c1, BRDN0000458261
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- RPS6KA2 (6196)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6196 | RPS6KA2 | ribosomal protein S6 kinase A2 | NM_021135.6 | 99.3% | 34.6% | (many diffs) |
| 2 | human | 6196 | RPS6KA2 | ribosomal protein S6 kinase A2 | XM_006715549.3 | 95.6% | 33.4% | (many diffs) |
| 3 | human | 6196 | RPS6KA2 | ribosomal protein S6 kinase A2 | NM_001006932.3 | 95.2% | 29.8% | (many diffs) |
| 4 | human | 6196 | RPS6KA2 | ribosomal protein S6 kinase A2 | NM_001318936.2 | 94.2% | 29.1% | (many diffs) |
| 5 | human | 6196 | RPS6KA2 | ribosomal protein S6 kinase A2 | NM_001318938.1 | 87.2% | 23% | (many diffs) |
| 6 | human | 6196 | RPS6KA2 | ribosomal protein S6 kinase A2 | NM_001318937.2 | 85.4% | 20.7% | (many diffs) |
| 7 | mouse | 20112 | Rps6ka2 | ribosomal protein S6 kinase... | NM_011299.4 | 87.2% | 33.5% | (many diffs) |
| 8 | mouse | 20112 | Rps6ka2 | ribosomal protein S6 kinase... | XM_006523199.3 | 84.1% | 30% | (many diffs) |
| 9 | mouse | 20112 | Rps6ka2 | ribosomal protein S6 kinase... | XM_006523198.3 | 84.1% | 30.3% | (many diffs) |
| 10 | mouse | 20112 | Rps6ka2 | ribosomal protein S6 kinase... | XM_006523200.1 | 74.4% | 20.4% | (many diffs) |
| 11 | mouse | 20112 | Rps6ka2 | ribosomal protein S6 kinase... | XM_006523201.1 | 74.4% | 20.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 96
- ORF end:
- 1053
- ORF length:
- 957
- Sequence:
-
1 CTATGATTTT ATTTTGACTG ATAGTGACCT GTTCGTTGCA ACAAATTGAT GAGCAATGCT 61 TTTTTATAAT GCCAACTTTG TACAAAAAAG TTGGCATGGA CCTGAGCATG AAGAAGTTCG 121 CCGTGCGCAG GTTCTTCTCT GTGTACCTGC GCAGGAAGTC GCGCTCCAAG AGCTCCAGCC 181 TGAGCCGGCT CGAGGAAGAA GGTGTCGTGA AGGAGATAGA CATCAGCCAT CATGTGAAGG 241 AGGGCTTTGA GAAGGCAGAT CCTTCCCAGT TTGAGCTGCT GAAGGTTTTA GGACAAGGAT 301 CCTATGGAAA GGTGTTCCTG GTGAGGAAGG TGAAGGGGTC CGACGCTGGG CAGCTCTACG 361 CCATGAAGGT CCTTAAGAAA GCCACCCTAA AAGTTCGGGA CCGAGTGAGA TCGAAGATGG 421 AGAGAGACAT CTTGGCAGAA GTGAATCACC CCTTCATTGT GAAGCTTCAT TATGCCTTTC 481 AGACGGAAGG AAAGCTCTAC CTGATCCTGG ACTTCCTGCG GGGAGGGGAC CTCTTCACCC 541 GGCTCTCCAA AGAGGTCATG TTCACGGAGG AGGATGTCAA GTTCTACCTG GCTGAGCTGG 601 CCTTGGCTTT AGACCATCTC CACAGCCTGG GGATCATCTA CAGAGATCTG AAGCCTGAGA 661 ACATCCTCCT GGATGAAGAG GGGCACATTA AGATCACAGA TTTCGGCCTG AGTAGGGAGG 721 CCATTGACCA CGACAAGAGA GCGTACTCCT TCTGCGGGAC GATCGAGTAC ATGGCGCCCG 781 AGGTGGTGAA CCGGCGAGGA CACACGCAGA GTGCCGACTG GTGGTCCTTC GGCGTGCTCA 841 TGTTTGAGAT GCTCACGGGG TCCCTGCCGT TCCAGGGAAA GGACCAGGAA AGGAGGccat 901 ggctctcatc ctcaaagcca agctggggat gccgcagttc ctcagtgggg aggcacagag 961 tttgctgcga gctctcttca aacggaaccc ctgcaaccgg ctgggtgctg gcattgacgg 1021 agtggaggaa attaagcgcc atcccttctt tgtgaccata gactggaaca cgctgtaccg 1081 gaaggagatc aagccaccgt tcaaaccagc agtgggcagg cctgaggaca ccttccactt 1141 tgaccccgag ttcacagcgc ggacgcccac agactctcct ggcgtccccc cgagtgcaaa 1201 cgctcatcac ctgtttagag gattcagctt tgtggcctca agcctgatcc aggagccctc 1261 acagcaagat ctgcacaaag tcccagttca cccaatcgtg cagcagttac acgggaacaa 1321 catccacttc accgatggct acgagatcaa ggaggacatc ggggtgggct cctactcagt 1381 gtgcaagcga tgtgtgcata aagccacaga caccgagtat gccgtgaaga tcattgataa 1441 gagcaagaga gacccctcgg aagagattga gatcctcctg cggtacggcc agcacccgaa 1501 catcatcacc CTCAAAGGAT GTTATGATGA TGCCAAGTTT GTGTACCTGG TAATGGAGCT 1561 GATGCGTGGT GGGGAGCTCC TGGACCGCAT CTTCCGGGCA GAGATACTTC TCGGAGCGCG 1621 AAGCCAGTGA CGTCCTGTGC ACCATCACCA AGACCATGGA CTACCTCCAT TCCCAGGGGG 1681 TGTCCATCGA GACCTGAAGC CGAGTAACAT CCTGTACAGG GATGAGTCGG GGAGCCCAGA 1741 ATCCATCCGA GTCTGCGACT TCGGCTTTGC CAAGCAGCTG CGCGCGGGGA ACGGGCTGCT 1801 CATGACACCC TGCTACACGG CCAATTTCGT GGCCCCGGAG GTCCTGAAGC GTCAAGGCTA 1861 TGATGCGGCG TGTGACATCT GGAGTTTGGG GATCCTGTTG TACACCATGC TGGCAGGATT 1921 TACCCCTTTT GCAAATGGGC CAGACGATAC CCCTGAGGAG ATTCTGGCGC GGATCGGCAG 1981 TGGGAAGTAT GCCCTTTCTG GGGGAAACTG GGACTCGATA TCTGACGCAG CTAAAGACGT 2041 CGTGTCCAAG ATGCTCCACG TGGACCCTCA TCAGCGCCTG ACGGCGATGC AAGTGCTCAA 2101 ACACCCGTGG GTGGTCAACA GAGAGTACCT GTCCCCAAAC CAGCTCAGCC GACAGGACGT 2161 GCACCTGGTG AAGGGCGCGA TGGCCGCCAC CTACTTTGCT CTAAACAGAA CACCTCAGGC 2221 CCCGCGGCTG GAGCCCGTGC TGTCGTCCAA CCTGGCTCAG CGCAGAGGCA TGAAGAGACT 2281 CACGTCCACG CGGTTGTACC CAACTTTCTT GTACAAAGTT GGCATTATAA GAAAGCATTG 2341 CTTATCAATT TGTTGCAACG AACAGGTCAC TATCAGTCAA AATAAAATCA TTATTGCCAT 2401 CCAGCCTGAA TCCCCCCT