Construct: ORF ccsbBroadEn_14876
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018932.1_s300c1, BRDN0000458228
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- VRK1 (7443)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7443 | VRK1 | VRK serine/threonine kinase 1 | NM_003384.3 | 99.9% | 52.2% | 619_620insA |
| 2 | human | 7443 | VRK1 | VRK serine/threonine kinase 1 | XM_017021624.2 | 99.6% | 52.4% | 619_620insA;1065_1066insAAG |
| 3 | human | 7443 | VRK1 | VRK serine/threonine kinase 1 | XM_017021625.1 | 99.4% | 52% | 1_6delATGAAA;625_626insA |
| 4 | human | 7443 | VRK1 | VRK serine/threonine kinase 1 | XM_006720247.4 | 94.2% | 49.2% | 619_620insA;1160_1231del |
| 5 | human | 7443 | VRK1 | VRK serine/threonine kinase 1 | XM_011537132.1 | 93.9% | 49.4% | 619_620insA;1065_1066insAAG;1157_1228del |
| 6 | human | 7443 | VRK1 | VRK serine/threonine kinase 1 | XR_001750539.2 | 64% | (many diffs) | |
| 7 | human | 7443 | VRK1 | VRK serine/threonine kinase 1 | XM_017021626.2 | 61.5% | 84.8% | 619_620insA;708_709ins121;732_733ins335 |
| 8 | mouse | 22367 | Vrk1 | vaccinia related kinase 1 | NM_001029844.1 | 84.4% | 47.9% | (many diffs) |
| 9 | mouse | 22367 | Vrk1 | vaccinia related kinase 1 | XM_017315046.1 | 84.4% | 47.9% | (many diffs) |
| 10 | mouse | 22367 | Vrk1 | vaccinia related kinase 1 | NM_001029843.1 | 80.7% | 45.6% | (many diffs) |
| 11 | mouse | 22367 | Vrk1 | vaccinia related kinase 1 | XM_011244103.1 | 79.8% | 45.2% | (many diffs) |
| 12 | mouse | 22367 | Vrk1 | vaccinia related kinase 1 | NM_011705.3 | 76.3% | 43.1% | (many diffs) |
| 13 | mouse | 22367 | Vrk1 | vaccinia related kinase 1 | XM_006515809.2 | 76.3% | 43.1% | (many diffs) |
| 14 | mouse | 22367 | Vrk1 | vaccinia related kinase 1 | XM_006515810.3 | 76.3% | 43.1% | (many diffs) |
| 15 | mouse | 22367 | Vrk1 | vaccinia related kinase 1 | XM_006515811.3 | 76.3% | 43.1% | (many diffs) |
| 16 | mouse | 22367 | Vrk1 | vaccinia related kinase 1 | XM_006515812.2 | 76.3% | 43.1% | (many diffs) |
| 17 | mouse | 22367 | Vrk1 | vaccinia related kinase 1 | XM_017315045.1 | 76.1% | 43.2% | (many diffs) |
| 18 | mouse | 22367 | Vrk1 | vaccinia related kinase 1 | XM_011244104.2 | 51.6% | 17.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 97
- ORF end:
- 727
- ORF length:
- 630
- Sequence:
-
1 GTAGGAGTTT TTTGACTGAT AGTGACCTGT TCGTTGCAAC AAATTGATGA GCAATGCTTT 61 TTTATAATGC CAACTTTGTA CAAAAAAGTT GGCACCATGC CTCGTGTAAA AGCAGCTCAA 121 GCTGGAAGAC AGAGCTCTGC AAAGAGACAT CTTGCAGAAC AATTTGCAGT TGGAGAGATA 181 ATAACTGACA TGGCAAAAAA GGAATGGAAA GTAGGATTAC CCATTGGCCA AGGAGGCTTT 241 GGCTGTATAT ATCTTGCTGA TATGAATTCT TCAGAGTCAG TTGGCAGTGA TGCACCTTGT 301 GTTGTAAAAG TGGAACCCAG TGACAATGGA CCTCTTTTTA CTGAATTAAA GTTCTACCAA 361 CGAGCTGCAA AACCAGAGCA AATTCAGAAA TGGATTCGTA CCCGTAAGCT GAAGTACCTG 421 GGTGTTCCTA AGTATTGGGG GTCTGGTCTA CATGACAAAA ATGGAAAAAG TTACAGGTTT 481 ATGATAATGG ATCGCTTTGG GAGTGACCTT CAGAAAATAT ATGAAGCAAA TGCCAAAAGG 541 TTTTCTCGGA AAACTGTCTT GCAGCTAAGC TTAAGAATTC TGGATATTCT GGAATATATT 601 CACGAGCATG AGTATGTGCA TGGAGATATC AAGGCCTCAA ATCTTCTTCT GAACTACAAG 661 AATCCTGACC AGGTGTACTT GGTAGATTAT GGCCTTGCTT ATCGGTACTG CCCAGAAAGG 721 AGTTCATAAA GAATACAAAG AAGACCCCAA AAGATGTCAC GATGGCACTA TTGAATTCAC 781 GAGCATCGAT GCACACAATG GCGTGGCCCC ATCAAGACGT GGTGATTTGG AAATACTTGG 841 TTATTGCATG ATCCAATGGC TTACTGGCCA TCTTCCTTGG GAGGATAATT TGAAAGATCC 901 TAAATATGTT AGAGATTCCA AAATTAGATA CAGAGAAAAT ATTGCAAGTT TGATGGACAA 961 ATGTTTTCCT GAGAAAAACA AACCAGGTGA AATTGCCAAA TACATGGAAA CAGTGAAATT 1021 ACTAGACTAC ACTGAAAAAC CTCTTTATGA AAATTTACGT GACATTCTTT TGCAAGGACT 1081 AAAAGCTATA GGAAGTAAGG ATGATGGCAA ATTGGACCTC AGTGTTGTGG AGAATGGAGG 1141 TTTGAAAGCA AAAACAATAA CAAAGAAGCG AAAGAAAGAA ATTGAAGAAA GCAAGGAACC 1201 TGGTGTTGAA GATACGGAAT GGTCAAACAC ACAGACAGAG GAGGCCATAC AGACCCGTTC 1261 AAGAACCAGA AAGAGAGTCC AGAAGTTGCC AACTTTCTTG TACAAAGTTG GCATTATAAG 1321 AAAGCATTGC TTATCAATTT GTTGCAACGA ACAGGTCACT ATCAGTCAAA ATAAAATCAT 1381 TATTGCCATC CAGCGAACTC CG