Construct: ORF ccsbBroadEn_14876

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018932.1_s300c1, BRDN0000458228
DNA Barcode:
None
Epitope Tag:
None
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
VRK1 (7443)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 7443 VRK1 VRK serine/threonine kinase 1 NM_003384.3 99.9% 52.2% 619_620insA
2 human 7443 VRK1 VRK serine/threonine kinase 1 XM_017021624.2 99.6% 52.4% 619_620insA;1065_1066insAAG
3 human 7443 VRK1 VRK serine/threonine kinase 1 XM_017021625.1 99.4% 52% 1_6delATGAAA;625_626insA
4 human 7443 VRK1 VRK serine/threonine kinase 1 XM_006720247.4 94.2% 49.2% 619_620insA;1160_1231del
5 human 7443 VRK1 VRK serine/threonine kinase 1 XM_011537132.1 93.9% 49.4% 619_620insA;1065_1066insAAG;1157_1228del
6 human 7443 VRK1 VRK serine/threonine kinase 1 XR_001750539.2 64% (many diffs)
7 human 7443 VRK1 VRK serine/threonine kinase 1 XM_017021626.2 61.5% 84.8% 619_620insA;708_709ins121;732_733ins335
8 mouse 22367 Vrk1 vaccinia related kinase 1 NM_001029844.1 84.4% 47.9% (many diffs)
9 mouse 22367 Vrk1 vaccinia related kinase 1 XM_017315046.1 84.4% 47.9% (many diffs)
10 mouse 22367 Vrk1 vaccinia related kinase 1 NM_001029843.1 80.7% 45.6% (many diffs)
11 mouse 22367 Vrk1 vaccinia related kinase 1 XM_011244103.1 79.8% 45.2% (many diffs)
12 mouse 22367 Vrk1 vaccinia related kinase 1 NM_011705.3 76.3% 43.1% (many diffs)
13 mouse 22367 Vrk1 vaccinia related kinase 1 XM_006515809.2 76.3% 43.1% (many diffs)
14 mouse 22367 Vrk1 vaccinia related kinase 1 XM_006515810.3 76.3% 43.1% (many diffs)
15 mouse 22367 Vrk1 vaccinia related kinase 1 XM_006515811.3 76.3% 43.1% (many diffs)
16 mouse 22367 Vrk1 vaccinia related kinase 1 XM_006515812.2 76.3% 43.1% (many diffs)
17 mouse 22367 Vrk1 vaccinia related kinase 1 XM_017315045.1 76.1% 43.2% (many diffs)
18 mouse 22367 Vrk1 vaccinia related kinase 1 XM_011244104.2 51.6% 17.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
97
ORF end:
727
ORF length:
630
Sequence:
1GTAGGAGTTT TTTGACTGAT AGTGACCTGT TCGTTGCAAC AAATTGATGA GCAATGCTTT
61TTTATAATGC CAACTTTGTA CAAAAAAGTT GGCACCATGC CTCGTGTAAA AGCAGCTCAA
121GCTGGAAGAC AGAGCTCTGC AAAGAGACAT CTTGCAGAAC AATTTGCAGT TGGAGAGATA
181ATAACTGACA TGGCAAAAAA GGAATGGAAA GTAGGATTAC CCATTGGCCA AGGAGGCTTT
241GGCTGTATAT ATCTTGCTGA TATGAATTCT TCAGAGTCAG TTGGCAGTGA TGCACCTTGT
301GTTGTAAAAG TGGAACCCAG TGACAATGGA CCTCTTTTTA CTGAATTAAA GTTCTACCAA
361CGAGCTGCAA AACCAGAGCA AATTCAGAAA TGGATTCGTA CCCGTAAGCT GAAGTACCTG
421GGTGTTCCTA AGTATTGGGG GTCTGGTCTA CATGACAAAA ATGGAAAAAG TTACAGGTTT
481ATGATAATGG ATCGCTTTGG GAGTGACCTT CAGAAAATAT ATGAAGCAAA TGCCAAAAGG
541TTTTCTCGGA AAACTGTCTT GCAGCTAAGC TTAAGAATTC TGGATATTCT GGAATATATT
601CACGAGCATG AGTATGTGCA TGGAGATATC AAGGCCTCAA ATCTTCTTCT GAACTACAAG
661AATCCTGACC AGGTGTACTT GGTAGATTAT GGCCTTGCTT ATCGGTACTG CCCAGAAAGG
721AGTTCATAAA GAATACAAAG AAGACCCCAA AAGATGTCAC GATGGCACTA TTGAATTCAC
781GAGCATCGAT GCACACAATG GCGTGGCCCC ATCAAGACGT GGTGATTTGG AAATACTTGG
841TTATTGCATG ATCCAATGGC TTACTGGCCA TCTTCCTTGG GAGGATAATT TGAAAGATCC
901TAAATATGTT AGAGATTCCA AAATTAGATA CAGAGAAAAT ATTGCAAGTT TGATGGACAA
961ATGTTTTCCT GAGAAAAACA AACCAGGTGA AATTGCCAAA TACATGGAAA CAGTGAAATT
1021ACTAGACTAC ACTGAAAAAC CTCTTTATGA AAATTTACGT GACATTCTTT TGCAAGGACT
1081AAAAGCTATA GGAAGTAAGG ATGATGGCAA ATTGGACCTC AGTGTTGTGG AGAATGGAGG
1141TTTGAAAGCA AAAACAATAA CAAAGAAGCG AAAGAAAGAA ATTGAAGAAA GCAAGGAACC
1201TGGTGTTGAA GATACGGAAT GGTCAAACAC ACAGACAGAG GAGGCCATAC AGACCCGTTC
1261AAGAACCAGA AAGAGAGTCC AGAAGTTGCC AACTTTCTTG TACAAAGTTG GCATTATAAG
1321AAAGCATTGC TTATCAATTT GTTGCAACGA ACAGGTCACT ATCAGTCAAA ATAAAATCAT
1381TATTGCCATC CAGCGAACTC CG

Download FASTA (ORF) (Full)

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