Construct: ORF ccsbBroadEn_14921
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018857.1_s300c1, BRDN0000458153
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- CDKL2 (8999)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 727758 | ROCK1P1 | Rho associated coiled-coil ... | NR_033770.1 | 19.8% | (many diffs) | |
| 2 | human | 727758 | ROCK1P1 | Rho associated coiled-coil ... | NR_160778.1 | 19.8% | (many diffs) | |
| 3 | human | 727758 | ROCK1P1 | Rho associated coiled-coil ... | NR_160777.1 | 18.2% | (many diffs) | |
| 4 | human | 6093 | ROCK1 | Rho associated coiled-coil ... | NM_005406.3 | 12% | 9.8% | (many diffs) |
| 5 | mouse | 19877 | Rock1 | Rho-associated coiled-coil ... | XM_006525725.1 | 11% | 9.5% | (many diffs) |
| 6 | mouse | 19877 | Rock1 | Rho-associated coiled-coil ... | NM_009071.2 | 11% | 9.5% | (many diffs) |
| 7 | mouse | 19877 | Rock1 | Rho-associated coiled-coil ... | XR_001782350.1 | 7.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 276
- ORF end:
- 714
- ORF length:
- 438
- Sequence:
-
1 NTNNNTNNNA NTNNNNNNNN NNNNNNNCTC CTATCTATCT CCGCGACCCC CCTTATTGAG 61 GTGCAGTACA CCCTCACACA TAAACAAATA CAGGAGTCAC AGACAGAGAT TTGGGTTTCA 121 GTAACTTCCC AGTCCCAGAG TGTAAAACGA CGGCCAGTCT TAAGCTCGGG CCCCAAATAA 181 TGATTTTATT TTGACTGATA GTGACCTGTT CGTTGCAACA AATTGATGAG CAATGCTTTT 241 TTATAATGCC AACTTTGTAC AAAAAAGTTG GCACCATGGC CTATATTTAT TTTCATTTTA 301 GTAAACTGTT TCACGTTAGA CCTGTAACCC AAGGAGATGT GTATAGAGCT GAAACTGAAG 361 AAATTCCTAA AATATTCCAG ATACTATATG CAAATGAAGG TGAATGTAGA AAAGATGTAG 421 AGATGGAACC AGTACAAGAA GCTGAAAAAA CTAATTTCCA AAATCACAAA GGCCATGAGT 481 TTATTCCTAC ACTCTACCAC TTTCCTGCCA ATTGTGATGC CTGTGCCAAA CCTCTCTGGC 541 ATGTTTTTAA GCCACCCCCT GCCCTAGAGT GTCGAAGATG CCATGTTAAG TGCCACAGAG 601 ATCACTTAGA TAAGAAAGAG GACTTAATTT GTCCATGTAA AGTAAGTTAT GATGTAACAT 661 CAGCAAGAGA TATGCTGCTG TTAGCATGTT CTCAGGATGA ACAAAAAAAT GGGTAACTCA 721 TTTAGTAAAG AAAATCCCTA AGAATCCACC ATCTGGTTTT GTTCGTGCTT CCCCTTTGCC 781 AACTTTCTTG TACAAAGTTG GCATTATAAG AAAGCATTGC TTATCAATTT GTTGCAACGA 841 ACAGGTCACT ATCAGTCAAA ATAAAATCAT TATTNNCNNN CNNNNNNNTN TCCCCTATAG 901 TGAGTCGTAT TACATGGTCA TAGCTGTTTC CTGGCAGCTC TGGCCCGTGT CTCAACTTTC 961 GTTTTTGTTT TCCTTTAGCT TATTTTAAAA CAAGCCTTTT CGGGTAGAGG CGATGAGGAG 1021 TACTCCCACC AGAGTCAGAT GGGTGGGGGG GTGATGAGCG ATAACTAGCT GGCGAGAGAA 1081 ATGCACTTTG TCATCCGCT