Transcript: Mouse XM_006525725.1

PREDICTED: Mus musculus Rho-associated coiled-coil containing protein kinase 1 (Rock1), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Rock1 (19877)
Length:
6057
CDS:
476..4537

Additional Resources:

NCBI RefSeq record:
XM_006525725.1
NBCI Gene record:
Rock1 (19877)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001146798 TTACATATTATAGCAATCGT pXPR_003 AGG 1206 30% 10 0.2264 Rock1 ROCK1 77595
Download CSV

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006525725.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000361522 CTACAGGTAGATTAGATTAAT pLKO_005 4759 3UTR 100% 15.000 21.000 N Rock1 n/a
2 TRCN0000361452 CTAGCAAAGAGAGTGATATTG pLKO_005 3714 CDS 100% 13.200 18.480 N Rock1 n/a
3 TRCN0000022900 CGGGAGTTACAAGATCAACTT pLKO.1 2990 CDS 100% 4.950 6.930 N Rock1 n/a
4 TRCN0000022902 CCGTGCAAAGTAAGTTACGAT pLKO.1 4316 CDS 100% 3.000 4.200 N Rock1 n/a
5 TRCN0000022957 CCTGATTATATCTCACCCGAA pLKO.1 1187 CDS 100% 2.160 3.024 N Gm5374 n/a
6 TRCN0000361453 TTCAATTGGTGAGGCATAAAT pLKO_005 744 CDS 100% 15.000 12.000 N Rock1 n/a
7 TRCN0000361521 CAACTTTCTAAGCAGATATAA pLKO_005 634 CDS 100% 15.000 10.500 N Rock1 n/a
8 TRCN0000022958 GCACCAGTTGTGCCTGATTTA pLKO.1 1532 CDS 100% 13.200 9.240 N Gm5374 n/a
9 TRCN0000022956 GCTGGATAAGTCTGGACATTT pLKO.1 1090 CDS 100% 13.200 9.240 N Gm5374 n/a
10 TRCN0000381922 GTGTCGAAGATGCCATGTTAA pLKO_005 4252 CDS 100% 13.200 9.240 N ROCK1P1 n/a
11 TRCN0000361455 TGTGGGATGCTACCTGATAAA pLKO_005 4567 3UTR 100% 13.200 9.240 N Rock1 n/a
12 TRCN0000381905 CAAGAGATATGCTGCTGTTAG pLKO_005 4347 CDS 100% 10.800 7.560 N ROCK1P1 n/a
13 TRCN0000022901 CCTGGTTTATGATTTGGATTT pLKO.1 583 CDS 100% 10.800 7.560 N Rock1 n/a
14 TRCN0000022954 GCCTGATTTAAGTAGTGACAT pLKO.1 1543 CDS 100% 4.950 3.465 N Gm5374 n/a
15 TRCN0000022899 GCAGAAATAATGAATCGCAAA pLKO.1 3491 CDS 100% 4.050 2.835 N Rock1 n/a
16 TRCN0000194943 CTAATGACTCTGTGCTCATTT pLKO.1 5999 3UTR 100% 1.320 0.924 N ROCK1 n/a
17 TRCN0000022903 GCAGTGTCTCAAATTGAGAAA pLKO.1 1910 CDS 100% 4.950 2.970 N Rock1 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006525725.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14921 pDONR223 100% 11% 9.5% None (many diffs) n/a
2 ccsbBroad304_14921 pLX_304 0% 11% 9.5% V5 (not translated due to prior stop codon) (many diffs) n/a
Download CSV