Construct: ORF ccsbBroadEn_14935
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018862.1_s300c1, BRDN0000458158
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- MAPKAPK2 (9261)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9261 | MAPKAPK2 | MAPK activated protein kina... | NM_032960.4 | 95.5% | 34% | (many diffs) |
| 2 | human | 9261 | MAPKAPK2 | MAPK activated protein kina... | NM_004759.5 | 85.8% | 36.7% | (many diffs) |
| 3 | human | 9261 | MAPKAPK2 | MAPK activated protein kina... | XM_005273353.3 | 85.8% | 36.6% | (many diffs) |
| 4 | human | 9261 | MAPKAPK2 | MAPK activated protein kina... | XM_017002810.1 | 79.9% | 15.6% | (many diffs) |
| 5 | mouse | 17164 | Mapkapk2 | MAP kinase-activated protei... | NM_008551.2 | 87.7% | 31.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 96
- ORF end:
- 546
- ORF length:
- 450
- Sequence:
-
1 TCATTTTATT TTGACTGATA GTGACCTGTT CGTTGCAACA AATTGATGAG CAATGCTTTT 61 TTATAATGCC AACTTTGTAC AAAAAAGTTG GCACCATGCT GTCCAACTCC CAGGGCCAGA 121 GCCCGCCGGT GCCGTTCCCC GCCCCGGCCC CGCCGCCGCC CCCGCAGCAG TTCCCGCAGT 181 TCCACGTCAA GTCCGGCCTG CAGATCAAGA AGAACGCCAT CATCGATGAC TACAAGGTCA 241 CCAGCCAGGT CCTGGGGCTG GGCATCAACG GCAAAGTTTT GCAGATCTTC AACAAGAGGA 301 CCCAGGAGAA ATTCGCCCTC AAAATGCTTC AGGACTGCCC CAAGGCCCGC AGGGAGGTGG 361 AGCTGCACTG GCGGGCCTCC CAGTGCCCGC ACATCGTACG GATCGTGGAT GTGTACGAGA 421 ATCTGTACGC AGGGAGGAAG TGCCTGCTGA TTGTCATGGA ATGTTTGGAC GGTGGAGAAC 481 TCTTTAGCCG AATCCCAGGA TCGAGGGAGA CCAGGCATTC ACAGAAAGAG AAGCATCCGA 541 AATCATGAAG AGCATCGGTG AGGCCATCCA GTATCTGCAT TCAATCAACA TTGCCCATCG 601 GGATGTCAAG CCTGAGAATC TCTTATACAC CTCCAAAAGG CCCAACGTCA TCCTGAAACT 661 CACTGACTTT GGCTTTGCCA AGGAAACCAC CAGCCACAAC TCTTTGACCA CTCCTTGTTA 721 TACACCGTAC TATGTGGCTC CAGAAGTGCT GGGTCCAGAG AAGTATGACA AGTCCTGTGA 781 CATGTGGTCC CTGGGTGTCA TCATGTACAT CCTGCTGTGT GGGTATCCCC CCTTCTACTC 841 CAACCACGGC CTTGCCATCT CTCCGGGCAT GAAGACTCGC ATCCGAATGG GCCAGTATGA 901 ATTTCCCAAC CCAGAATGGT CAGAAGTATC AGAGGAAGTG AAGATGCTCA TTCGGAATCT 961 GCTGAAAACA GAGCCCACCC AGAGAATGAC CATCACCGAG TTTATGAACC ACCCTTGGAT 1021 CATGCAATCA ACAAAGGTCC CTCAAACCCC ACTGCACACC AGCCGGGTCC TGAAGGAGGA 1081 CAAGGAGCGG TGGGAGGATG TCAAGGAGGA GATGACCAGT GCCTTGGCCA CAATGCGCGT 1141 TGACTACGAG CAGATCAAGA TAAAAAAGAT TGAAGATGCA TCCAACCCTC TGCTGCTGAA 1201 GAGGCGGAAG AAAGCTCGGG CCCTGGAGGC TGCGGCTCTG GCCCACTTGC CAACTTTCTT 1261 GTACAAAGTT GGCATTATAA GAAAGCATTG CTTATCAATT TGTTGCAACG AACAGGTCAC 1321 TATCAGTCAA AATAAAATCA TTATTGCCAT CCAGCGAAGC AAGA