Transcript: Human NM_032960.4

Homo sapiens MAPK activated protein kinase 2 (MAPKAPK2), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-08-27
Taxon:
Homo sapiens (human)
Gene:
MAPKAPK2 (9261)
Length:
3091
CDS:
326..1528

Additional Resources:

NCBI RefSeq record:
NM_032960.4
NBCI Gene record:
MAPKAPK2 (9261)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001146296 GTGTACGAGAATCTGTACGC pXPR_003 AGG 383 32% 2 0.9009 MAPKAPK2 MAPKAPK2 77208
2 BRDN0001147193 GATCTTCAACAAGAGGACCC pXPR_003 AGG 256 21% 1 0.827 MAPKAPK2 MAPKAPK2 77206
3 BRDN0001148536 TGTTATACACCGTACTATGT pXPR_003 GGG 686 57% 5 0.6542 MAPKAPK2 MAPKAPK2 77207
4 BRDN0001145417 CCGGACTTGACGTGGAACTG pXPR_003 CGG 135 11% 1 -0.4874 MAPKAPK2 MAPKAPK2 77209
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_032960.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000195312 CCGAGTTTATGAACCACCCTT pLKO.1 1275 CDS 100% 2.640 3.696 N MAPKAPK2 n/a
2 TRCN0000002285 CCAGCACTCGATTGTTGTAAA pLKO.1 3021 3UTR 100% 13.200 9.240 N MAPKAPK2 n/a
3 TRCN0000219652 TGGTTTGTTAGAGGGTATTTG pLKO.1 1878 3UTR 100% 13.200 9.240 N MAPKAPK2 n/a
4 TRCN0000002286 AGAAAGAGAAGCATCCGAAAT pLKO.1 802 CDS 100% 10.800 7.560 N MAPKAPK2 n/a
5 TRCN0000195074 CTGATTGTCATGGAATGTTTG pLKO.1 728 CDS 100% 10.800 7.560 N MAPKAPK2 n/a
6 TRCN0000219651 TCGTGGATGTGTACGAGAATC pLKO.1 684 CDS 100% 10.800 7.560 N MAPKAPK2 n/a
7 TRCN0000194963 CCATCCAGTATCTGCATTCAA pLKO.1 843 CDS 100% 5.625 3.938 N MAPKAPK2 n/a
8 TRCN0000002282 CTCTTTGACCACTCCTTGTTA pLKO.1 979 CDS 100% 5.625 3.938 N MAPKAPK2 n/a
9 TRCN0000002283 GACTACGAGCAGATCAAGATA pLKO.1 1421 CDS 100% 5.625 3.938 N MAPKAPK2 n/a
10 TRCN0000002284 GCAATCAACAAAGGTCCCTCA pLKO.1 1303 CDS 100% 2.160 1.512 N MAPKAPK2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_032960.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07381 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_07381 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000476256 ATCCGTGCATGCCAGTGTACTACA pLX_317 30.8% 100% 100% V5 n/a
4 ccsbBroadEn_14935 pDONR223 100% 95.5% 34% None (many diffs) n/a
5 ccsbBroad304_14935 pLX_304 0% 95.5% 34% V5 (not translated due to prior stop codon) (many diffs) n/a
6 TRCN0000470986 GCGGATCGTTTCACATTGGTTTGT pLX_317 36.2% 95.5% 34% V5 (not translated due to prior stop codon) (many diffs) n/a
7 TRCN0000489713 AGGCAGTCTCACCTTTAGTACACT pLX_317 30.7% 86.1% 84.1% V5 (not translated due to prior stop codon) (many diffs) n/a
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