Construct: ORF ccsbBroadEn_15028
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018926.1_s300c1, BRDN0000458222
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PRKD2 (25865)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25865 | PRKD2 | protein kinase D2 | NM_001079880.1 | 99.6% | 29.6% | (many diffs) |
| 2 | human | 25865 | PRKD2 | protein kinase D2 | NM_001079881.1 | 99.6% | 29.6% | (many diffs) |
| 3 | human | 25865 | PRKD2 | protein kinase D2 | NM_016457.4 | 99.6% | 29.6% | (many diffs) |
| 4 | human | 25865 | PRKD2 | protein kinase D2 | NM_001079882.1 | 81.7% | 12.4% | (many diffs) |
| 5 | human | 25865 | PRKD2 | protein kinase D2 | XM_005258716.2 | 81.7% | 12.4% | (many diffs) |
| 6 | mouse | 101540 | Prkd2 | protein kinase D2 | NM_001252458.1 | 87.8% | 28.6% | (many diffs) |
| 7 | mouse | 101540 | Prkd2 | protein kinase D2 | NM_178900.4 | 87.8% | 28.6% | (many diffs) |
| 8 | mouse | 101540 | Prkd2 | protein kinase D2 | XR_391036.1 | 60.2% | (many diffs) | |
| 9 | mouse | 101540 | Prkd2 | protein kinase D2 | XM_006539444.1 | 55.2% | 11.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 97
- ORF end:
- 1237
- ORF length:
- 1140
- Sequence:
-
1 ACGGATTTAT TTTGACTGAT AGTGACCTGT TCGTTGCAAC AAATTGATGA GCAATGCTTT 61 TTTATAATGC CAACTTTGTA CAAAAAAGTT GGCACCATGG CCACCGCCCC CTCTTATCCC 121 GCCGGGCTCC CTGGCTCTCC CGGGCCGGGG TCTCCTCCGC CCCCCGGCGG CCTAGAGCTG 181 CAGTCGCCGC CACCGCTACT GCCCCAGATC CCGGCCCCGG GTTCCGGGGT CTCCTTTCAC 241 ATCCAGATCG GGCTGACCCG CGAGTTCGTG CTGTTGCCCG CCGCCTCCGA GCTGGCTCAT 301 GTGAAGCAGC TGGCCTGTTC CATCGTGGAC CAGAAGTTCC CTGAGTGTGG CTTCTACGGC 361 CTTTACGACA AGATCCTGCT TTTCAAACAT GACCCCACGT CGGCCAACCT CCTGCAGCTG 421 GTGCGCTCGT CCGGAGACAT CCAGGAGGGC GACCTGGTGG AGGTGGTGCT GTCGGCCTCG 481 GCCACCTTCG AGGACTTCCA GATCCGCCCG CACGCCCTCA CGGTGCACTC CTATCGGGCG 541 CCTGCCTTCT GTGATCACTG CGGGGAGATG CTCTTCGGCC TAGTGCGCCA GGGCCTCAAG 601 TGCGATGGCT GCGGGCTGAA CTACCACAAG CGCTGTGCCT TCAGCATCCC CAACAACTGT 661 AGTGGGGCCC GCAAACGGCG CCTGTCATCC ACGTCTCTGG CCAGTGGCCA CTCGGTGCGC 721 CTCGGCACCT CCGAGTCCCT GCCCTGCACG GCTGAAGAGC TGAGCCGTAG CACCACCGAA 781 CTCCTGCCTC GCCGTCCCCC GTCATCCTCT TCCTCCTCTT CTGCCTCATC GTATACGGGC 841 CGCCCCATTG AGCTGGACAG ATGCTGCTCC CCAAGGTCAA ggtgccgcac accttcctca 901 tccacagcta tacacggccc accgtttgcc aggcttgcaa gaaactcctc aagggcctct 961 tccggcaggg cctgcaatgc aaagactgca agtttaactg tcacaaacgc tgcgccaccc 1021 gcgtccctaa tgactgcctg ggggaggccc ttatcaatgg agatgtgccg atggaggagg 1081 ccaccgattt cagcgaggct gacaagagcg ccctcatgga tgagtcagag gactccggtg 1141 tcatccctgg ctcccactca gagaatgcgc tccacgccag tgaggaggag gaaggcgagg 1201 gaggcaaggc ccagagctcc ctggggtaca tccccctaat gagggtggtg caatcggtgc 1261 gacacacgac gcggaaatcc agcaccacgc tgcgggaggg ttgggtggtt cattacagca 1321 acaaggacac gctgagaaag cggcactatt ggcgcctgga ctgcaagtgt atcacgctct 1381 tccagaacaa cacgaccaac agatactata aggaaattcc gctgtcagaa atcctcacgg 1441 tggagtccgc ccagaacttc agccttgtgc cgccgggcac caacccacac tgctttgaga 1501 tcgtcactgc caatgccacc tacttcgtgg gcgagatgcc tggcgggact ccgggtgggc 1561 caagtgggca gggggctgag gccgcccggg gctgggagac agccatccgc caggccctga 1621 tgcccgtcat ccttcaggac gcacccagcg ccccaggcca cgcgccccac agacaagctt 1681 ctctgagcat ctctgtgtcc aacagtcaga tccaagagaa tgtggacatt gccactgtct 1741 accagatctt ccctgacgaa gtgctgggct cagggcagtt tggagtggtc tatggaggaa 1801 aacaccggaa gacaggccgg gacgtggcag ttaaggtcat tgacaaactg cgcttcccta 1861 ccaagcagga gagccagctc cggaatgaag tggccattct gcagagcctg cggcatcccg 1921 ggatcgtgaa cctggagtgc atgttcgaga cgcctgagaa agtgttTGTG TGGATGAGAA 1981 AGCTGCATGG GGGACAGGTG GGAGATGATC CTGTCCAGTG AGAAGGGCCG GCTGCCTGAG 2041 CGCCTCACCA AGTTCCTCAT CACCCAGATC CTGGTGGCTT NGAGACACCT TCACTTCAAG 2101 AACATTGTCC ACTGTGACTT GAAACCAGAA AACGTGTTGC TGGCATCAGC AGACCCATTT 2161 CCTCAGGTGA AGCTGTGTGA CTTTGGCTTT GCTCGCATCA TCGGCGAGAA GTCGTTCCGC 2221 CGCTCAGTGG TGGGCACGCC GGCCTACCTG GCACCCGAGG TGCTGCTCAA CCAGGGCTAC 2281 AACCGCTCGC TGGACATGTG GTCAGTGGGC GTGATCATGT ACGTCAGCCT CAGCGGCACC 2341 TTCCCTTTCA ACGAGGATGA GGACATCAAT GACCAGATCC AGAACGCCGC CTTCATGTAC 2401 CCGGCCAGCC CCTGGAGCCA CATCTCAGCT GGAGCCATTG ACCTCATCAA CAACCTGCTG 2461 CAGGTGAAGA TGCGCAAACG CTACAGCGTG GACAAATCTC TCAGCCACCC CTGGTTACAG 2521 GAGTACCAGA CGTGGCTGGA CCTCCGAGAG CTGGAGGGGA AGATGGGAGA GCGATACATC 2581 ACGCATGAGA GTGACGACGC GCGCTGGGAG CAGTTTGCAG CAGAGCATCC GCTGCCTGGG 2641 TCTGGGCTGC CCACGGACAG GGATCTCGGT GGGGCCTGTC CACCACAGGA CCACGACATG 2701 CAGGGGCTGG CGGAGCGCAT CAGTGTTCTC TTGCCAACTT TCTTGTACAA AGTTGGCATT 2761 ATAAGAAAGC ATTGCTTATC AATTTGTTGC AACGAACAGG TCACTATCAG TCAAAATAAA 2821 ATCATTATTG CCATCCCAGC TGATATT