Construct: ORF ccsbBroadEn_15152

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018928.1_s300c1, BRDN0000458224
DNA Barcode:
None
Epitope Tag:
None
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
PEAK1 (79834)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 79834 PEAK1 pseudopodium enriched atypi... NM_024776.4 90.7% 6.2% (many diffs)
2 human 79834 PEAK1 pseudopodium enriched atypi... XM_005254670.2 90.7% 6.2% (many diffs)
3 human 79834 PEAK1 pseudopodium enriched atypi... XM_005254671.2 90.7% 6.2% (many diffs)
4 human 79834 PEAK1 pseudopodium enriched atypi... XM_005254673.2 90.7% 6.2% (many diffs)
5 human 79834 PEAK1 pseudopodium enriched atypi... XM_005254674.2 90.7% 6.2% (many diffs)
6 human 79834 PEAK1 pseudopodium enriched atypi... XM_005254675.4 90.7% 6.2% (many diffs)
7 human 79834 PEAK1 pseudopodium enriched atypi... XM_011522033.2 90.7% 6.2% (many diffs)
8 human 79834 PEAK1 pseudopodium enriched atypi... XM_011522034.2 90.7% 6.2% (many diffs)
9 human 79834 PEAK1 pseudopodium enriched atypi... XM_011522035.2 90.7% 6.2% (many diffs)
10 human 79834 PEAK1 pseudopodium enriched atypi... XM_011522036.2 90.7% 6.2% (many diffs)
11 human 79834 PEAK1 pseudopodium enriched atypi... XM_011522037.2 90.7% 6.2% (many diffs)
12 human 79834 PEAK1 pseudopodium enriched atypi... XM_011522038.2 90.7% 6.2% (many diffs)
13 human 79834 PEAK1 pseudopodium enriched atypi... XM_011522039.2 90.7% 6.2% (many diffs)
14 human 79834 PEAK1 pseudopodium enriched atypi... XM_024450056.1 90.7% 6.2% (many diffs)
15 human 79834 PEAK1 pseudopodium enriched atypi... XM_024450057.1 90.7% 6.2% (many diffs)
16 human 79834 PEAK1 pseudopodium enriched atypi... XM_024450058.1 90.7% 6.2% (many diffs)
17 human 79834 PEAK1 pseudopodium enriched atypi... XM_024450059.1 90.7% 6.2% (many diffs)
18 human 79834 PEAK1 pseudopodium enriched atypi... XM_024450060.1 90.7% 6.2% (many diffs)
19 human 79834 PEAK1 pseudopodium enriched atypi... XM_024450061.1 90.7% 6.2% (many diffs)
20 human 79834 PEAK1 pseudopodium enriched atypi... XM_024450062.1 90.7% 6.2% (many diffs)
21 human 79834 PEAK1 pseudopodium enriched atypi... XM_024450063.1 90.7% 6.2% (many diffs)
22 human 79834 PEAK1 pseudopodium enriched atypi... XM_024450064.1 90.7% 6.2% (many diffs)
23 human 79834 PEAK1 pseudopodium enriched atypi... XM_011522040.1 69.3% 7.9% (many diffs)
24 human 79834 PEAK1 pseudopodium enriched atypi... XM_011522041.1 51.6% 10.3% (many diffs)
25 human 79834 PEAK1 pseudopodium enriched atypi... XM_011522042.2 41.5% 3.7% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
159
ORF end:
570
ORF length:
411
Sequence:
1NNANGNNNNT NNNNNGNNNN ANNGNGANNN AACGACGTCC AGTTTTTAGT TCGGGCCCCA
61AATAAGATTC TATTTGGACT GAAAGTTACT NTTNNTTGCA ANAAATTGAT GAGCAATGCT
121TTTTTATAAT GCCAANTTNN TACAAAAAAN TNGGCACCAT GTCTGCTTGT AACACCTTTA
181CTGAACATGT TTGGAAACCT GGTGAATGCA AGAATTGCTT TAAACCTAAA AGTTTGCACC
241AGCTTCCCCC AGACCCTGAG AAGGCACCCA TCACCCATGG CAATGTGAAA ACTAATGCCA
301ATCACAGTAA CAACCACCGC ATCAGGAACA CGGGCAATTT CCGGCCTCCT GTGGCTAAAA
361AACCCACTAT AGCTGTGAAG CCCACTATGA TAGTGGCAGA TGGGCAAAGT ATATGTGGTG
421AGCTTAGCAT CCAAGAACAC TGTGAGAACA AACCTGTCAT CATAGGGTGG AACCGAAAAG
481CGGATCCTCA TTTCAGACGC CAAAGGCATC CTCCAGTGTC TGCTCTGGGG CCCCCGCGAA
541GATCTCTTCC AGACTTTCAC CGCCTGCCCT AGCCTAGTAC AGAGGAACAC CCTGCTCCAA
601AACTGGCTAG ACATCAAGCG AACACTGCTC ATGATCAAGT TTGCTGAGAA GTCCCTGGAC
661AGGGAAGGTG GAATCAGCCT TGAGGACTGG CTTTGTGCTC AGTATTTGGC TTTTGCCACT
721ACAGACTCCC TCAGTTGTAT TGTGAAAATT CTGCAGCACC GTTTGCCAAC TTTCTTGTAC
781AAAGTTGGCA TTATAAGAAA GCNNNNNTTN NCNANNTGTT GCAACGAACA GGTCACTATC
841AGTCAAAATA AAATCATTAT TGCCATCNNG NNNGATTCAA AGTTGGCATT ATAAGAAAGC
901ANCCCCCTGC TTATCAATTT GTTGCAACGA ACAGGTCACT ATCAGTCAAA ATAAAATCAT
961TATTTGCCAT CCAGGCTGAT ATCCCCGTAT AGTGAGTCGT ATTACATGGA TCATAGCTGT
1021TTCCTGGCAG CTCTGGGCCC GTGTCTCAAT Ccacatcacc agattcttct ttaacagaag
1081aatcacgttc tgagacagcc agtagtttat cccagaagat ttgtaatggg ggattatctc
1141ctggtaaccc aggagattct aaggacatga aggaaattga gcccaattat gaaagtccct
1201ctagtaataa tcaggataaa gattcatcac aggcttccaa aagctcaata aaagttccag
1261agacccacaa agcagtcctt gctctccgat tagaagagaa agatggcaag attgctgtac
1321aaactgagaa ggaagaaagt aaagcctcta cagatgttgc tgggcaagca gtaaccataa
1381accttgtccc cacagaagag caagcaaaac cttaccgagt tgtgaacctg gaacagccat
1441tgtgcaagcc atatactgtc gtggatgtgt cagcagccat ggccagtgag cacctcgagg
1501gccctgttaa cagccccaag acaaaaagct catcctctac tccaaactct ccagttacat
1561catcttcatt gacaccagga caaataagtg cccatttcca aaaatccagt gcaattcgat
1621accaagaagt atggacttct agcaccagtc cacgacaaaa gatacctaaa gtagaactaa
1681ttactagtgg aactggacca aatgttcctc caaggaaaaa ctgtcacaaa tcagcaccta
1741catcacccac agctacaaac atttcctcca aaaccatccc tgttaagtca cctaatttgt
1801ctgaaattaa atttaatagt tataacaatg ctggtatgcc accttttcca attatcattc
1861atgacgagcc aacttatgct cggagttcca aaaatgctat caaagttccc attgttatca
1921atccaaatgc atatgacaat ctagctatct acaaaagttt tctgggaaca agtggagaac
1981tctcagtgaa ggaaaaaacc acaagtgtaa taagccatac ttatgaagaa atagaaacag
2041aaagcaaagt gcctgataac accactagca aaaccactga ctgtcttcaa actaaagggt
2101tttcaaacag cacagagcat aaaaggggct cagtggctca gaaggttcaa gagtttaaca
2161actgtctcaa cagaggtcag tcttcaccac agagaagcta tagttccagc cacagctccc
2221cagcaaagat ccagagagcc actcaagagc ctgtggccaa aatagaaggc actcaggagt
2281ctcagatggt gggcagcagc agcaccagag agaaagcaag cacagtgctt tctcagattg
2341tggcttcaat ccaaccccca cagtctcctc cagaaacacc tcaatctggc cctaaagctt
2401gcagtgtgga agagctttat gccattcctc cagatgctga tgttgctaag agcacaccta
2461agagtacgcc agtccggccc aaatctctct ttacatctca gcctagtggt gaggctgaag
2521cacctcagac cacagacagt cctaccacca aagtacagaa agacccatcc ataaagccag
2581tcaccccctc tccctccaaa ttagtgacta gcccccaaag tgagccacca gctccctttc
2641ccccgccacg ctctacttct tctccttacc atgcaggtaa ccttttgcag aggcatttca
2701ccaactggac caagccaacc agccctacca ggtcaacaga agctgaatca gttttgcact
2761ctgaaggcag caggcgggca gctgatgcaa aacctaagcg ctggatatca tttaaaagct
2821tcttccgccg tcggaaaaca gatgaggagg atgacaaaga gaaagagcga gagaaaggga
2881aactggtggg cctggatggc acagtcattc acatgctgcc tcctcctcca gttcagcgcc
2941atcactggtt cacagaggcg aaaggagagt ccagtgagaa accagccatt gtcttcatgt
3001acaggtgcga ccctgctcaa ggccagctca gtgtggatca gagcaaggct aggacagacc
3061aggcagcagt catggagaag ggtagagcag agaatgcatt actacaggac tcagagaaga
3121agaggagtca ttcttctcca tcacagattc ctaaaaagat tctcagtcac atgacccatg
3181aagtaacaga ggatttttct cctcgggatc caagaactgt tgttgggaag caagatggca
3241ggggctgcac ttcagtcaca acagcattgt ccctacctga actggaaagg gaagatggaa
3301aagaagacat ttcagatcct atggacccga acccttgtag tgcaacatac agcaacttag
3361ggcaatctag agcagccatg atacctccca agcagccacg acagcccaag ggagctgtgg
3421acgatgccat cgcctttgga gggaaaacag accaagaagc acccaatgct tcccaaccta
3481caccaccccc actgccaaag aagatgatca taagagccaa tacagagcca atctccaagg
3541acctccaaaa atccatggaa agtagtcttt gtgtcatggc taatcccacc tatgatatcg
3601accccaactg ggatgccagc agtgctggtt cttccatcag ctatgaactc aaaggactgg
3661acattgagtc ttatgactcc ttggaaaggc ctttgcgcaa ggagagacct gtcccctcag
3721cagcaaacag catttccagc ttaaccactc tcagtattaa ggatagattt tccaacagca
3781tggaatccct ctccagccgg cgtgggccct cttgcagaca gggccgaggc atccagaagc
3841cgcagagaca agcactttat cgaggacttg agaatcggga ggaagtagtg ggtaaaatcc
3901gaagccttca tacagatgcc ttgaagaaac tggctgttaa atgcgaagac cttttcatgg
3961ctgggcagaa agaccagctc cgttttggag tggacagctg gtcagacttc aggctaacca
4021gtgacaaacc atgttgtgag gcaggtgatg cggtttacta tactgcttca tatgcaaaag
4081atccacttaa taactatgca gtcaagatct gtaagagcaa agctaaagaa tctcagcagt
4141attatcacag cttggctgtc cggcagagtc tggctgtcca ttttaacatt cagcaggact
4201gtggtcattt ccttgctgaa gtccctaacc gtctgcttcc ctgggaggat ccagatgacc
4261ctgaaaagga tgaggatgac atggaagaga ctgaagaaga cgccaaagga gaaacggatg
4321ggaaaaaccc aaagccctgt tctgaagcag catcatccca gaaagagaat cagggagtca
4381tgagcaagaa gcagaggagc cacgttgtgg tcatcaccag ggaggttcca tgtcttactg
4441tggctgattt tgtgcgagac tctctggccc agcatgggaa aagccctgat ttgtatgaga
4501ggcaggtgtg tctgctgctc ttacagctat gctctggtct tgagcacctc aaaccctacc
4561atgtcactca ctgcgatcta cgcctagaga acctgctact tgtccactac cagcctgggg
4621ggactgccca aggctttggg cctgcagagc ccagccccac ctcatcttat cccactaggc
4681ttatagtgag caacttctct caggccaagc agaagagcca tctggtggac cccgagatcc
4741tccgggacca gtctcgcctt gccccagaga tcataacagc tacccagtat aaaaagtgtg
4801atgagttcca gacaggcatc ctcatctatg agatgctgca cctacccaac ccctttgatg
4861agaacccaga gctgaaggag agggaataca cacgagcaga cctgcctcgc atcccattcc
4921gctcccccta ctcccggggt ctgcagcagc tggccagctg cctcctgaat cccaaccctt
4981ctgagcggat cctcatttca gacgccaaag gcatcctcca gtgtctgctc tggggccccc
5041gcgaagatct cttccagact ttcaccgcct gccctagcct agtacagagg aacaccctgc
5101tccaaaactg gctagacatc aagcgaacac tgctcatgat caagtttgct gagaagtccc
5161tggacaggga aggtggaatc agccttgagg actggctttg tgctcagtat ttggcttttg
5221ccactacaga ctccctcagt tgtattgtga aaattctgca gcaccgtttg ccaactttct
5281tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac gaac

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