Transcript: Human XM_011522039.2

PREDICTED: Homo sapiens pseudopodium enriched atypical kinase 1 (PEAK1), transcript variant X18, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
PEAK1 (79834)
Length:
12328
CDS:
1088..6328

Additional Resources:

NCBI RefSeq record:
XM_011522039.2
NBCI Gene record:
PEAK1 (79834)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001145890 ATGACGAGCCAACTTATGCT pXPR_003 CGG 1848 35% 11 0.8276 PEAK1 PEAK1 75853
2 BRDN0001147244 GAAGTGATTAGTAATGAAGG pXPR_003 GGG 662 13% 11 0.6761 PEAK1 PEAK1 75854
3 BRDN0001147666 GTGCAAGCCATATACTGTCG pXPR_003 TGG 1429 27% 11 0.2487 PEAK1 PEAK1 75855
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011522039.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000088549 CCCTGTTAAGTCACCTAATTT pLKO.1 2836 CDS 100% 15.000 21.000 N Peak1 n/a
2 TRCN0000195001 CCCTGTTAAGTCACCTAATTT pLKO.1 2836 CDS 100% 15.000 21.000 N PEAK1 n/a
3 TRCN0000037443 CCACAAGTGTAATAAGCCATA pLKO.1 3057 CDS 100% 4.050 5.670 N PEAK1 n/a
4 TRCN0000333784 CCACAAGTGTAATAAGCCATA pLKO_005 3057 CDS 100% 4.050 5.670 N PEAK1 n/a
5 TRCN0000195688 CCTAAGCGCTGGATATCATTT pLKO.1 3851 CDS 100% 13.200 9.240 N PEAK1 n/a
6 TRCN0000196723 GCATAGAACATGTGCACATAA pLKO.1 8486 3UTR 100% 13.200 9.240 N PEAK1 n/a
7 TRCN0000197185 GAAGATCTCTTCCAGACTTTC pLKO.1 6101 CDS 100% 10.800 7.560 N PEAK1 n/a
8 TRCN0000199724 GCCATGTTCCTAAGCCTTATG pLKO.1 1467 CDS 100% 10.800 7.560 N PEAK1 n/a
9 TRCN0000037439 CCCATGAAGTAACAGAGGATT pLKO.1 4233 CDS 100% 4.950 3.465 N PEAK1 n/a
10 TRCN0000333785 CCCATGAAGTAACAGAGGATT pLKO_005 4233 CDS 100% 4.950 3.465 N PEAK1 n/a
11 TRCN0000037442 CCTCTAGTAATAATCAGGATA pLKO.1 2256 CDS 100% 4.950 3.465 N PEAK1 n/a
12 TRCN0000037441 CGGTTTACTATACTGCTTCAT pLKO.1 5109 CDS 100% 4.950 3.465 N PEAK1 n/a
13 TRCN0000333865 CGGTTTACTATACTGCTTCAT pLKO_005 5109 CDS 100% 4.950 3.465 N PEAK1 n/a
14 TRCN0000037440 CCCACTATGATAGTGGCAGAT pLKO.1 1310 CDS 100% 0.405 0.284 N PEAK1 n/a
15 TRCN0000074123 CGCCTGTAATCCCAACACTTT pLKO.1 10111 3UTR 100% 4.950 2.475 Y GJD4 n/a
16 TRCN0000166650 CGCCTGTAATCCCAACACTTT pLKO.1 10111 3UTR 100% 4.950 2.475 Y C9orf85 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011522039.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15152 pDONR223 17.6% 90.7% 6.2% None (many diffs) n/a
2 ccsbBroad304_15152 pLX_304 0% 90.7% 6.2% V5 (not translated due to prior stop codon) (many diffs) n/a
3 TRCN0000491580 AATTGGATTGTCGATGTGTTGATT pLX_317 5.1% 90.7% 6.2% V5 (not translated due to prior stop codon) (many diffs) n/a
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