Construct: ORF ccsbBroadEn_15254
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018831.1_s300c1, BRDN0000458127
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- NEK7 (140609)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 140609 | NEK7 | NIMA related kinase 7 | NM_133494.3 | 99% | 15.2% | (many diffs) |
| 2 | human | 140609 | NEK7 | NIMA related kinase 7 | XM_017000344.1 | 96.4% | 14.8% | (many diffs) |
| 3 | human | 140609 | NEK7 | NIMA related kinase 7 | XM_017000345.1 | 96.4% | 14.8% | (many diffs) |
| 4 | human | 140609 | NEK7 | NIMA related kinase 7 | XM_017000346.1 | 84.6% | 16.9% | (many diffs) |
| 5 | human | 140609 | NEK7 | NIMA related kinase 7 | XM_017000347.1 | 67.3% | 20.3% | (many diffs) |
| 6 | human | 140609 | NEK7 | NIMA related kinase 7 | XM_017000348.1 | 67.3% | 20.3% | (many diffs) |
| 7 | human | 140609 | NEK7 | NIMA related kinase 7 | XM_011509209.1 | 52.5% | 28.7% | (many diffs) |
| 8 | mouse | 59125 | Nek7 | NIMA (never in mitosis gene... | NM_021605.4 | 90.4% | 14.9% | (many diffs) |
| 9 | mouse | 59125 | Nek7 | NIMA (never in mitosis gene... | NM_001311148.1 | 60.2% | 22.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 81
- ORF end:
- 219
- ORF length:
- 138
- Sequence:
-
1 AGATAGTGAC CTGTTCGTTG CAACAAATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT 61 TGTACAAAAA AGTTGGCACC ATGGATGAGC AATCACAAGG AATGCAAGGG CCACCTGTTC 121 CTCAGTTCCA ACCACAGAAG GCCTTACGAC CGGATATGGG CTATAATACA TTAGCCAACT 181 TTCGAATAGA AAAGAAAATT GGTCGCGGAC AATTTAGTTG AAGTTTATAG AGCAGCCTGT 241 CTCTNGGATG GAGTACCAGT AGCTTTAAAA AAAGTGCAGA TATTTGNNTT AATGGATGCC 301 AAAGCACGTG CTGATTGCAT CAAAGAAATA GATCTTCTTA AGCAACTCAA CCATCCAAAT 361 GTAATAAAAT ATTATGCATC ATTCATTGAA GATAATGAAC TAAACATAGT TTTGGAACTA 421 GCAGATGCTG GCGACCTATC CAGAATGATC AAGCATTTTA AGAAGCAAAA GAGGCTAATT 481 CCTGAAAGAA CTGTTTGGAA GTATTTTGTT CAGCTTTGCA GTGCATTGGA ACACATGCAT 541 TCTCGAAGAG TCATGCATAG AGATATAAAA CCAGCTAATG TGTTCATTAC AGCCACTGGG 601 GTGGTAAAAC TTGGAGATCT TGGGCTTGGC CGGTTTTTCA GCTCAAAAAC CACAGCTGCA 661 CATTCTTTAG TTGGTACGCC TTATTACATG TCTCCAGAGA GAATACATGA AAATGGGATA 721 CAACTTCAAA TCTGACATCT GGTCTCTTGG CTGTCTACTA TATGAGATGG CTGCATTACA 781 AAGTCCTTTC TATGGTGACA AAATGAATTT ATACTCACTG TGTAAGNAGA TAGAACAGTG 841 TGACTACCCA CCTCTNNCTT CAGATCACTA TTCAGAAGNA CTCCGACAGT TAGTTAATAT 901 GTGCATCAAC CCAGATCCAG AGAAGCGACC AGACGTCACC TATGTTTATG ACGTAGCAAA 961 GAGGATGCAT GCATGCACTG CAAGCAGCTT GCCAACTTTC TTGTACAAAG TTGGCATTAT 1021 AAGAAAGCAT TGCTTATCAA TTTGTTGCAA CGAACAGGTC ACTATCAGTC AAAATAAAAT 1081 CATTATTGCC ATCCAGCGAG CTGGC