Transcript: Human XM_017000345.1

PREDICTED: Homo sapiens NIMA related kinase 7 (NEK7), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
NEK7 (140609)
Length:
4243
CDS:
413..1345

Additional Resources:

NCBI RefSeq record:
XM_017000345.1
NBCI Gene record:
NEK7 (140609)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001149173 ATAGCCCATATCCGGTCGTA pXPR_003 AGG 67 7% 4 0.3872 NEK7 NEK7 77741
2 BRDN0001162196 TCTCGAAGAGTCATGCATAG pXPR_003 AGG 500 54% 7 0.0844 NEK7 NEK7 77740
3 BRDN0001145539 ACCTAAAGAATGTGCAGCTG pXPR_003 TGG 598 64% 8 0.0017 NEK7 NEK7 77739
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017000345.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000199087 CGACCGGATATGGGCTATAAT pLKO.1 479 CDS 100% 15.000 21.000 N NEK7 n/a
2 TRCN0000001967 CTTTAGTTGGTACGCCTTATT pLKO.1 1020 CDS 100% 13.200 18.480 N NEK7 n/a
3 TRCN0000199150 CACGTGCTGATTGCATCAAAG pLKO.1 636 CDS 100% 10.800 15.120 N NEK7 n/a
4 TRCN0000001966 GCGGACAATTTAGTGAAGTTT pLKO.1 537 CDS 100% 5.625 7.875 N NEK7 n/a
5 TRCN0000194884 CCTATGTTTATGACGTAGCAA pLKO.1 1293 CDS 100% 3.000 4.200 N NEK7 n/a
6 TRCN0000196722 GAGTCATGCATAGAGATATAA pLKO.1 903 CDS 100% 15.000 10.500 N NEK7 n/a
7 TRCN0000001969 GAAGAGTGTAACCAAAGTAAT pLKO.1 1360 3UTR 100% 13.200 9.240 N NEK7 n/a
8 TRCN0000199184 CCACAGCTGCACATTCTTTAG pLKO.1 1005 CDS 100% 10.800 7.560 N NEK7 n/a
9 TRCN0000194964 CTATATGAGATGGCTGCATTA pLKO.1 1112 CDS 100% 10.800 7.560 N NEK7 n/a
10 TRCN0000001968 TCAGATCACTATTCAGAAGAA pLKO.1 1214 CDS 100% 4.950 3.465 N NEK7 n/a
11 TRCN0000010671 TGGCTGCATTACAAAGTCCTT pLKO.1 1122 CDS 100% 2.640 1.848 N NEK7 n/a
12 TRCN0000194795 CCAGCTAATGTGTTCATTACA pLKO.1 926 CDS 100% 0.563 0.394 N NEK7 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017000345.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000487923 GATATGCTGATTTGGGTTGCTATC pLX_317 27.1% 97.4% 97.4% V5 (not translated due to prior stop codon) 370_393del n/a
2 TRCN0000470840 GATCCCGGCAACAACTACTCGAAC pLX_317 36% 96.9% 14.8% V5 (not translated due to prior stop codon) (many diffs) n/a
3 ccsbBroadEn_15254 pDONR223 100% 96.4% 14.8% None (many diffs) n/a
4 ccsbBroad304_15254 pLX_304 0% 96.4% 14.8% V5 (not translated due to prior stop codon) (many diffs) n/a
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