Construct: ORF ccsbBroadEn_15268
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018872.1_s300c1, BRDN0000458168
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- NEK10 (152110)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 152110 | NEK10 | NIMA related kinase 10 | NM_001031741.3 | 89.5% | 36.4% | (many diffs) |
| 2 | human | 152110 | NEK10 | NIMA related kinase 10 | NM_001304384.1 | 87.6% | 35.7% | (many diffs) |
| 3 | human | 152110 | NEK10 | NIMA related kinase 10 | XM_024453374.1 | 58.2% | 23.1% | (many diffs) |
| 4 | human | 152110 | NEK10 | NIMA related kinase 10 | XM_017005772.2 | 38.2% | 15% | (many diffs) |
| 5 | human | 152110 | NEK10 | NIMA related kinase 10 | XM_006713001.3 | 37.2% | 14.7% | (many diffs) |
| 6 | human | 152110 | NEK10 | NIMA related kinase 10 | XM_017005771.2 | 37.2% | 14.7% | (many diffs) |
| 7 | human | 152110 | NEK10 | NIMA related kinase 10 | XM_017005769.1 | 37.2% | 14.6% | (many diffs) |
| 8 | human | 152110 | NEK10 | NIMA related kinase 10 | XM_017005770.2 | 37.2% | 14.6% | (many diffs) |
| 9 | human | 152110 | NEK10 | NIMA related kinase 10 | XM_017005768.1 | 37.2% | 14.6% | (many diffs) |
| 10 | human | 152110 | NEK10 | NIMA related kinase 10 | XM_017005767.2 | 36.6% | 14.4% | (many diffs) |
| 11 | human | 152110 | NEK10 | NIMA related kinase 10 | XM_024453373.1 | 36.6% | 14.4% | (many diffs) |
| 12 | human | 152110 | NEK10 | NIMA related kinase 10 | XM_006712997.3 | 36.1% | 14.1% | (many diffs) |
| 13 | human | 152110 | NEK10 | NIMA related kinase 10 | XM_017005766.2 | 36.1% | 14.1% | (many diffs) |
| 14 | human | 152110 | NEK10 | NIMA related kinase 10 | NM_152534.4 | 35.7% | 14% | (many diffs) |
| 15 | human | 152110 | NEK10 | NIMA related kinase 10 | XM_017005761.2 | 35.7% | 14% | (many diffs) |
| 16 | human | 152110 | NEK10 | NIMA related kinase 10 | XM_017005762.2 | 35.7% | 14% | (many diffs) |
| 17 | human | 152110 | NEK10 | NIMA related kinase 10 | XM_017005763.1 | 35.7% | 14% | (many diffs) |
| 18 | human | 152110 | NEK10 | NIMA related kinase 10 | XM_017005764.1 | 35.7% | 14% | (many diffs) |
| 19 | human | 152110 | NEK10 | NIMA related kinase 10 | XM_017005765.1 | 35.7% | 14% | (many diffs) |
| 20 | human | 152110 | NEK10 | NIMA related kinase 10 | XM_006712998.2 | 34.7% | 14.1% | (many diffs) |
| 21 | human | 152110 | NEK10 | NIMA related kinase 10 | XM_006712999.3 | 34.5% | 14.2% | (many diffs) |
| 22 | human | 152110 | NEK10 | NIMA related kinase 10 | XM_017005773.1 | 22% | 17.4% | (many diffs) |
| 23 | human | 152110 | NEK10 | NIMA related kinase 10 | XR_001740034.1 | 19.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 98
- ORF end:
- 653
- ORF length:
- 555
- Sequence:
-
1 AATGATTTTA TTTTGACTGA TAGTGACCTG TTCGTTGCAA CAAATTGATG AGCAATGCTT 61 TTTTATAATG CCAACTTTGT ACAAAAAAGT TGGCACCATG GTTCCTGTAC AGCCTGCAGA 121 ACCCCCCGAG GTACTGAAGA GTGAGCCGTA TGGGGAGAAG GCTGATGTCT GGGCAGTAGG 181 CTGCATCCTT TATCAGATGG CGACTTTGAG TCCCCCCTTC TACAGCACTA ACATGCTGTC 241 CTTGGCTACA AAAATAGTGG AGGCGGTATA TGAACCAGTG CCAGAAGGTA TCTACTCTGA 301 AAAAGTAACA GACACCATCA GCAGGTGCCT CACTCCTGAT GCGGAAGCTC GTCCAGATAT 361 TGTAGAAGTC AGTTCGATGA TATCAGATGT CATGATGAAA TATTTAGACA ACTTATCTAC 421 ATCCCAGTTG TCCTTGGAAA AGAAGCTAGA ACGGGAACGA AGACGCACAC AAAGGTATTT 481 TATGGAAGCC AACCGGAACA CCGTCACATG TCACCATGAG CTGGCTGTTC TATCTCACGA 541 GACCTTTGAG AAGGCAAGTT TGAGTAGCAN CAGCNNNGGA NCAGCCANCC TGAAAANTGA 601 ACTTTCANAA AGCGCAGACC TGCCCCCTGN AGGCTTTCCA GGCCTCCTAT GGTAAAGACG 661 AAGACAGGGC CTGTGACGAA ATCCTGTCAG ATGATAACTT CAACCTGGAA AATGCTGAGA 721 AAGATACATA TTCAGAGGTA GATGATGAAT TGGACATTTC GGATAACTCC AGCAGCTCCA 781 GTTCAAGCCC TCTGAAAGNA TCTACATTCA ACATTTTAAA GAGAAGTTTT AGTGCTTCAG 841 GAGGAGAAAG ACANNCCCAA ANNAGGGACT TCACTGGAGG AACAGGATCA AGACCAAGAC 901 CAGACAGACA GCATCAGCAG GAATTGCTGT GTCCCAGAGG AAAGTGCGTC AGATCAGTGA 961 TCCTATTCAG CAGATATTAA TTCAGCTGCA CAAAATAATC TATATCACAC AGCTTCCTCC 1021 AGCTTTGCAC CACAATTTGA AAAGAAGGGT TATAGAGAGA TTCAAGAAAT CCCTCTTCAG 1081 CCAGCAGAGT AACCCTTGTA ATTTGAAATC TGAAATTAAA AAGTTATCTC AGGGATCTCC 1141 AGAACCGATT GAGCCCAACT TTTTCACAGC AGATTACCAT TTATTACATC GTTCATCCGG 1201 TGGAAACAGC CTGTCCCCAA ATGACCCTAC AGGTTTACCA ACCAGCATTG AATTGGAGGA 1261 AGGAATAACA TATGAACAGA TGCAGACTGT GATTGAAGAA GTCCTTGAGG AAAGTGGCTA 1321 TTACAATTTT ACATCTAACA GGTATCATTC CTATCCATGG GGGACCAAGA ATCACCCAAC 1381 CAAAAGATTG CCAACTTTCT TGTACAAAGT TGGCATTATA AGAAAGCATT GCTTATCAAT 1441 TTGTTGCAAC GAACAGGTCA CTATCAGTCA AAATAAAATC ATTATTGCCA TCCAGGCTGA 1501 TATCCCCCTA