Transcript: Human NM_152534.4

Homo sapiens NIMA related kinase 10 (NEK10), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-05-01
Taxon:
Homo sapiens (human)
Gene:
NEK10 (152110)
Length:
4215
CDS:
314..3832

Additional Resources:

NCBI RefSeq record:
NM_152534.4
NBCI Gene record:
NEK10 (152110)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001146401 TAGAGGCTGAGTTAGTGACA pXPR_003 GGG 2952 84% 32 1.094 NEK10 NEK10 75633
2 BRDN0001162522 GGGAACGAAGACGCACACAA pXPR_003 AGG 2436 69% 27 0.4319 NEK10 NEK10 75635
3 BRDN0001149193 AAGACAATCCCAAACAAGGT pXPR_003 AGG 2830 80% 30 0.089 NEK10 NEK10 75634
4 BRDN0001162335 GTCAGATGATAACTTCAACC pXPR_003 TGG 2668 76% 28 -0.0332 NEK10 NEK10 75636
Download CSV

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_152534.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000002287 CACAGCAGATTACCATTTATT pLKO.1 3607 CDS 100% 15.000 21.000 N NEK10 n/a
2 TRCN0000196316 GAATTGGACATTTCGGATAAC pLKO.1 3026 CDS 100% 10.800 15.120 N NEK10 n/a
3 TRCN0000195225 CTGACACCAAACAACATTATG pLKO.1 2279 CDS 100% 13.200 10.560 N NEK10 n/a
4 TRCN0000195038 CTTTATCAGATGGCGACTTTG pLKO.1 2468 CDS 100% 10.800 8.640 N NEK10 n/a
5 TRCN0000002290 GCTCGTCCAGATATTGTAGAA pLKO.1 2627 CDS 100% 4.950 3.960 N NEK10 n/a
6 TRCN0000002289 CATTGCCAGAACACATTATAT pLKO.1 4026 3UTR 100% 15.000 10.500 N NEK10 n/a
7 TRCN0000002291 GCAGAGTAACCCTTGTAATTT pLKO.1 3526 CDS 100% 15.000 10.500 N NEK10 n/a
8 TRCN0000195480 CCCTTCTACAGCACTAACATG pLKO.1 2495 CDS 100% 4.950 3.465 N NEK10 n/a
9 TRCN0000196372 GCTGTGATTCTTCGAAATCTC pLKO.1 3293 CDS 100% 4.950 3.465 N NEK10 n/a
10 TRCN0000196534 GCTTAGCTCTTCGATACTTAC pLKO.1 2229 CDS 100% 10.800 6.480 N NEK10 n/a
11 TRCN0000002288 CCTCTTGACCTGCTTCTGAAA pLKO.1 3191 CDS 100% 4.950 2.970 N NEK10 n/a
12 TRCN0000027639 GTCCAGATATTGTAGAAGTTA pLKO.1 2631 CDS 100% 5.625 3.938 N Nek10 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_152534.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13285 pDONR223 100% 39.9% 39.7% None (many diffs) n/a
2 ccsbBroad304_13285 pLX_304 0% 39.9% 39.7% V5 (many diffs) n/a
3 TRCN0000477492 AAGCTATCTCTCTTCCCTAAGAAG pLX_317 20.4% 39.9% 39.7% V5 (many diffs) n/a
4 ccsbBroadEn_15268 pDONR223 100% 35.7% 14% None (many diffs) n/a
5 ccsbBroad304_15268 pLX_304 0% 35.7% 14% V5 (not translated due to prior stop codon) (many diffs) n/a
6 TRCN0000468798 TCTAGAATGAACCAGTCTGATATC pLX_317 25% 35.7% 14% V5 (not translated due to prior stop codon) (many diffs) n/a
Download CSV