Transcript: Human XM_024453374.1

PREDICTED: Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X22, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
NEK10 (152110)
Length:
8856
CDS:
2782..4920

Additional Resources:

NCBI RefSeq record:
XM_024453374.1
NBCI Gene record:
NEK10 (152110)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_024453374.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000002287 CACAGCAGATTACCATTTATT pLKO.1 4695 CDS 100% 15.000 21.000 N NEK10 n/a
2 TRCN0000196316 GAATTGGACATTTCGGATAAC pLKO.1 4285 CDS 100% 10.800 15.120 N NEK10 n/a
3 TRCN0000195225 CTGACACCAAACAACATTATG pLKO.1 3538 CDS 100% 13.200 10.560 N NEK10 n/a
4 TRCN0000195038 CTTTATCAGATGGCGACTTTG pLKO.1 3727 CDS 100% 10.800 8.640 N NEK10 n/a
5 TRCN0000002290 GCTCGTCCAGATATTGTAGAA pLKO.1 3886 CDS 100% 4.950 3.960 N NEK10 n/a
6 TRCN0000002289 CATTGCCAGAACACATTATAT pLKO.1 5114 3UTR 100% 15.000 10.500 N NEK10 n/a
7 TRCN0000002291 GCAGAGTAACCCTTGTAATTT pLKO.1 4614 CDS 100% 15.000 10.500 N NEK10 n/a
8 TRCN0000195480 CCCTTCTACAGCACTAACATG pLKO.1 3754 CDS 100% 4.950 3.465 N NEK10 n/a
9 TRCN0000196534 GCTTAGCTCTTCGATACTTAC pLKO.1 3488 CDS 100% 10.800 6.480 N NEK10 n/a
10 TRCN0000027639 GTCCAGATATTGTAGAAGTTA pLKO.1 3890 CDS 100% 5.625 3.938 N Nek10 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_024453374.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15268 pDONR223 100% 58.2% 23.1% None (many diffs) n/a
2 ccsbBroad304_15268 pLX_304 0% 58.2% 23.1% V5 (not translated due to prior stop codon) (many diffs) n/a
3 TRCN0000468798 TCTAGAATGAACCAGTCTGATATC pLX_317 25% 58.2% 23.1% V5 (not translated due to prior stop codon) (many diffs) n/a
4 ccsbBroadEn_13285 pDONR223 100% 55.4% 55.2% None (many diffs) n/a
5 ccsbBroad304_13285 pLX_304 0% 55.4% 55.2% V5 (many diffs) n/a
6 TRCN0000477492 AAGCTATCTCTCTTCCCTAAGAAG pLX_317 20.4% 55.4% 55.2% V5 (many diffs) n/a
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