Construct: ORF ccsbBroadEn_15297
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018937.1_s300c1, BRDN0000458233
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- KSR2 (283455)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 283455 | KSR2 | kinase suppressor of ras 2 | XM_011538225.3 | 99.8% | 25.5% | (many diffs) |
| 2 | human | 283455 | KSR2 | kinase suppressor of ras 2 | NM_173598.6 | 87.1% | 22.3% | (many diffs) |
| 3 | human | 283455 | KSR2 | kinase suppressor of ras 2 | XM_011538224.3 | 86.9% | 22.3% | (many diffs) |
| 4 | human | 283455 | KSR2 | kinase suppressor of ras 2 | XM_017019208.2 | 83.8% | 22.6% | (many diffs) |
| 5 | human | 283455 | KSR2 | kinase suppressor of ras 2 | XM_011538226.3 | 65.1% | 28.5% | (many diffs) |
| 6 | human | 283455 | KSR2 | kinase suppressor of ras 2 | XM_017019210.2 | 61.8% | 6.9% | (many diffs) |
| 7 | human | 283455 | KSR2 | kinase suppressor of ras 2 | XM_011538229.3 | 52.2% | 34.2% | (many diffs) |
| 8 | human | 283455 | KSR2 | kinase suppressor of ras 2 | XM_017019209.2 | 51.5% | 34.1% | (many diffs) |
| 9 | mouse | 333050 | Ksr2 | kinase suppressor of ras 2 | NM_001312914.1 | 77.4% | 20.2% | (many diffs) |
| 10 | mouse | 333050 | Ksr2 | kinase suppressor of ras 2 | XM_006530393.3 | 77.2% | 20.3% | (many diffs) |
| 11 | mouse | 333050 | Ksr2 | kinase suppressor of ras 2 | XM_006530394.3 | 69.5% | 7.6% | (many diffs) |
| 12 | mouse | 333050 | Ksr2 | kinase suppressor of ras 2 | XM_006530395.3 | 61.8% | 8.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 99
- ORF end:
- 744
- ORF length:
- 645
- Sequence:
-
1 AGAGGGTTTT TTTTTGACTG ATAGTGACCT GTTCGTTGCA ACAAATTGAT GAGCAATGCT 61 TTTTTATAAT GCCAACTTTG TACAAAAAAG TTGGCACCAT GACGGATGAA CAGGTGTGCG 121 AGACTGTGGA GAAATACGGA GCCAACCGGG AGGAGTGTGC CCGCCTCAAC GCCTCCCTCT 181 CCTGCCTCAG GAATGTCCAC ATGTCAGGAG GCAACCTTTC CAAACAAGAC TGGACCATCC 241 AGTGGCCCAC CACAGAGACG GGGAAGGAGA ACAATCCCGT GTGCCCCCCG GAGCCCACCC 301 CGTGGATCCG CACCCATCTC TCCCAGAGCC CCAGGGTCCC GTCCAAGTGC GTCCAGCACT 361 ATTGTCACAC CAGCCCCACT CCCGGGGCCC CTGTGTACAC CCACGTGGAC AGGCTTACCG 421 TGGACGCCTA CCCGGGCTTG TGCCCGCCCC CGCCACTGGA GTCGGGCCAC CGTTCCCTGC 481 CCCCATCGCC CCGGCAGCGG CACGCGGTCC GCACCCCGCC GCGCACCCCC AACATCGTCA 541 CCACCGTGAC CCCGCCGGGC ACGCCGCCCA TGAGGAAGAA GAACAAGCTG AAGCCCCCGG 601 GGACCCCACC GCCCTCCTCC CGAAAACTGA TACACTTGAT CCCGGGATTC ACCGCGCTGC 661 ATCGGAGCAA ATCCCACGAG TTCCAGCTGG GGCACCGCGT GGACGAGGCC CACACGCCCA 721 AAGCCAAGAA GAGAGCAAAC CCTTGAACCT CAAGATCCAC AGCAGCGTAG GCAGCTGCGA 781 GAACATCCCC TCTCAGCAGC GCTTCccgct gctgtccgag cgctccctcc gctccttctt 841 tgtgggacac gcacctttcc tgccttccac ccctcctgtt cacactgagg ccaacttctc 901 tgcaaacaca ctgtcagtgc cacgctggtc cccgcagatc cctcgcagag atctcggcaa 961 ctccatcaag cacaggtttt ccaccaagta ctggatgtct cagacgtgca cagtctgtgg 1021 gaaagggatg ctttttggcc tcaagtgtaa aaactgcaag ttaaagtgcc acaacaaatg 1081 caccaaagaa gccccaccct gtcatcttct gatcatccac cgaggagatc cagcaaggtt 1141 agtccggaca gagtccgttc cgtgtgacat caacaaccct ctacggaagc cacctcgcta 1201 ttcagacctg cacatcagtc agacgctccc caaaaccaac aaaatcaaca aggaccacat 1261 ccctgtccct taccagccag actccagcag caacccctcc tccacgacgt cctccacgcc 1321 ctcctcgcca gcaccccccc tccctcctag tgccacgccg ccttctcccc tacacccttc 1381 cccacagtgc acacggcagc agaagaactt caacctgcca gcatcccact actacaaata 1441 caagcagcag ttcatcttcc cagatgtggt gccggtgccg gagacgccga cccgggcgcc 1501 ccaggtcatc ctgcatccgg tgacctcgaa tccaatcttg gaaggaaatc cattacttca 1561 aattgaagtg gagccaacgt cggagaatga agaggtccat gatgaggccg aagagtcaga 1621 ggatgacttc gaggagatga acctgtccct cctctcggcc cggagcttcc cacgcaaggc 1681 cagccagacc agcatcttcc ttcaggagtg ggacatcccc tttgagcagc tggagatcgg 1741 cgagctcatt ggaaagggcc gctttgggca agtgtaccac ggccgctggc atggcgaggt 1801 ggccatccgg ctgattgaca ttgagaggga caacgaggac cagctcaagg ccttcaagcg 1861 ggaggtgatg gcctacaggc agacacggCA TGAGAACGTG GTGCTTTTCA TGGGTGCCTG 1921 CATGAGCCCG CCTCACCTGG CCCATCATCA CCAGCCTCTG TAAGGGACGG ACGCTCTATT 1981 CCGTTGTGAG GGATGCCAAA ATCGTTTTGG ATGTCAACAA AACCAGGCAG ATTGCTCAAG 2041 AAATTGTGAA GGGCATGGGC TACCTCCACG CCAAGGGAAT CCTACACAAG GACCTCAAGT 2101 CAAAGAACGT CTTCTATGAC AACGGCAAAG TGGTCATCAC GGACTTTGGA CTCTTCAGCA 2161 TTTCTGGGGT GCTGCAGGCT GGCAGGCGGG AGGACAAACT GCGCATCCAG AATGGCTGGC 2221 TATGCCACCT GGCACCAGAG ATCATCCGCC AGCTGTCCCC CGACACAGAG GAGGATAAGC 2281 TCCCCTTCTC CAAGCACTCT GACGTCTTTG CCCTTGGCAC AATCTGGTAT GAACTCCACG 2341 CCAGGGAATG GCCTTTCAAG ACCCAACCAG CAGAGGCAAT AATCTGGCAA ATGGGCACAG 2401 GCATGAAACC CAACCTCAGC CAGATTGGCA TGGGAAAAGA AATCTCGGAC ATTCTTCTCT 2461 TCTGCTGGGC CTTTGAACAA GAAGAGAGAC CTACCTTCAC CAAGCTCATG GACATGCTGG 2521 AGAAACTGCC AAAGCGAAAC CGTCGCCTGT CTCACCCTGG ACATTTCTGG AAGTCTGCAG 2581 AGCTGTTGCC AACTTTCTTG TACAAAGTTG GCATTATAAG AAAGCATTGC TTATCAATTT 2641 GTTGCAACGA ACAGGTCACT ATCAGTCAAA ATAAAATCAT TATTGCCATC CAGCGTGCTC 2701 TG