Transcript: Human XM_011538225.3

PREDICTED: Homo sapiens kinase suppressor of ras 2 (KSR2), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
KSR2 (283455)
Length:
17784
CDS:
1126..3615

Additional Resources:

NCBI RefSeq record:
XM_011538225.3
NBCI Gene record:
KSR2 (283455)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001148172 TTAAATAGTTACCTCCTCGG pXPR_003 TGG 1025 41% 8 0.6566 KSR2 KSR2 76426
2 BRDN0001145237 AATTGAAGTGGAGCCAACGT pXPR_003 CGG 1480 59% 13 0.4586 KSR2 KSR2 76427
3 BRDN0001147424 CCACGTGGACAGGCTTACCG pXPR_003 TGG 319 13% 4 0.1162 KSR2 KSR2 76425
Download CSV

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011538225.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000382261 AGTTAAAGTGCCACAACAAAT pLKO_005 2087 CDS 100% 13.200 18.480 N KSR2 n/a
2 TRCN0000335901 CAGCCAGGGTGATGCGTTATT pLKO_005 3840 3UTR 100% 13.200 18.480 N KSR2 n/a
3 TRCN0000335903 GTAAGGGACGGACGCTCTATT pLKO_005 2987 CDS 100% 13.200 18.480 N KSR2 n/a
4 TRCN0000335902 TACGGAAGCCACCTCGCTATT pLKO_005 2210 CDS 100% 10.800 15.120 N KSR2 n/a
5 TRCN0000195374 CTTGGCACAATCTGGTATGAA pLKO.1 3340 CDS 100% 5.625 7.875 N KSR2 n/a
6 TRCN0000007063 GCAGAGGCAATAATCTGGCAA pLKO.1 3397 CDS 100% 2.640 3.696 N KSR2 n/a
7 TRCN0000381917 CCAACCAGCAGAGGCAATAAT pLKO_005 3390 CDS 100% 15.000 10.500 N KSR2 n/a
8 TRCN0000199112 CCAAGCACTCTGACGTCTTTG pLKO.1 3317 CDS 100% 10.800 7.560 N KSR2 n/a
9 TRCN0000195569 CGTTCCGTGTGACATCAACAA pLKO.1 2184 CDS 100% 4.950 3.465 N KSR2 n/a
10 TRCN0000007065 GAGAATGAAGAGGTCCATGAT pLKO.1 2611 CDS 100% 4.950 3.465 N KSR2 n/a
11 TRCN0000199619 GCCTCAGGAATGTCCACATGT pLKO.1 1211 CDS 100% 4.950 3.465 N KSR2 n/a
12 TRCN0000335815 GCCTCAGGAATGTCCACATGT pLKO_005 1211 CDS 100% 4.950 3.465 N KSR2 n/a
13 TRCN0000007062 CCTCAAGTCAAAGAACGTCTT pLKO.1 3120 CDS 100% 4.050 2.835 N KSR2 n/a
14 TRCN0000199136 CGTAGGCAGCTGCGAGAACAT pLKO.1 1794 CDS 100% 1.650 1.155 N KSR2 n/a
15 TRCN0000335816 CGTAGGCAGCTGCGAGAACAT pLKO_005 1794 CDS 100% 1.650 1.155 N KSR2 n/a
16 TRCN0000199943 GCAAGGTTAGTCCGGACAGAG pLKO.1 2161 CDS 100% 1.350 0.945 N KSR2 n/a
17 TRCN0000194721 CCATTACTTCAAATTGAAGTG pLKO.1 2578 CDS 100% 0.405 0.284 N KSR2 n/a
18 TRCN0000382366 TGTGCGAGACTGTGGAGAAAT pLKO_005 1142 CDS 100% 13.200 7.920 N KSR2 n/a
19 TRCN0000007061 CCAGGCAGATTGCTCAAGAAA pLKO.1 3050 CDS 100% 5.625 3.375 N KSR2 n/a
20 TRCN0000022631 CAAACCCTTGAACCTCAAGAT pLKO.1 1764 CDS 100% 4.950 2.970 N Gm1390 n/a
21 TRCN0000007064 CAAGTTAAAGTGCCACAACAA pLKO.1 2085 CDS 100% 4.950 2.970 N KSR2 n/a
22 TRCN0000363377 GCAAGTTAAAGTGCCACAATA pLKO_005 2084 CDS 100% 13.200 7.920 N Ksr2 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011538225.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13499 pDONR223 100% 99.9% 100% None 153G>C;858C>T n/a
2 ccsbBroad304_13499 pLX_304 0% 99.9% 100% V5 153G>C;858C>T n/a
3 TRCN0000475094 TCTTCTCCGAATTGTACACATATC pLX_317 14.9% 99.9% 100% V5 153G>C;858C>T n/a
4 ccsbBroadEn_15297 pDONR223 56.8% 99.8% 25.5% None (many diffs) n/a
5 ccsbBroad304_15297 pLX_304 0% 99.8% 25.5% V5 (not translated due to prior stop codon) (many diffs) n/a
6 TRCN0000469219 CAGATTCATAAGAGAGCCTCTTCA pLX_317 12.4% 99.8% 25.5% V5 (not translated due to prior stop codon) (many diffs) n/a
Download CSV