Construct: ORF ccsbBroadEn_15527

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013817.1_s300c1, BRDN0000386734
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PFKP (5214)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5214 PFKP phosphofructokinase, platelet NM_002627.5 99.8% 100% 483G>A;1281A>G;1794C>T
2 human 5214 PFKP phosphofructokinase, platelet XM_005252466.4 96.7% 96.5% (many diffs)
3 human 5214 PFKP phosphofructokinase, platelet NM_001323067.1 96.1% 95.4% (many diffs)
4 human 5214 PFKP phosphofructokinase, platelet NM_001323071.2 95% 95.1% (many diffs)
5 human 5214 PFKP phosphofructokinase, platelet NM_001323072.1 95% 95.1% (many diffs)
6 human 5214 PFKP phosphofructokinase, platelet NM_001345944.1 95% 95.1% (many diffs)
7 human 5214 PFKP phosphofructokinase, platelet XM_024448038.1 95% 95.1% (many diffs)
8 human 5214 PFKP phosphofructokinase, platelet XM_005252465.4 93.7% 93.8% (many diffs)
9 human 5214 PFKP phosphofructokinase, platelet NM_001323068.2 93.3% 93.4% (many diffs)
10 human 5214 PFKP phosphofructokinase, platelet NM_001242339.1 92.1% 85.9% (many diffs)
11 human 5214 PFKP phosphofructokinase, platelet XM_006717449.1 89.2% 89.3% (many diffs)
12 human 5214 PFKP phosphofructokinase, platelet NM_001323069.2 78.3% 78.4% 0_1ins507;774A>G;1287C>T
13 human 5214 PFKP phosphofructokinase, platelet NM_001323073.1 72.3% 72.4% 0_1ins648;633A>G;1146C>T
14 human 5214 PFKP phosphofructokinase, platelet NM_001323074.1 72.3% 72.4% 0_1ins648;633A>G;1146C>T
15 human 5214 PFKP phosphofructokinase, platelet NM_001323070.1 65.8% 65.9% (many diffs)
16 mouse 56421 Pfkp phosphofructokinase, platelet NM_019703.4 82.1% 88.2% (many diffs)
17 mouse 56421 Pfkp phosphofructokinase, platelet NM_001291071.1 80.1% 85.8% (many diffs)
18 mouse 56421 Pfkp phosphofructokinase, platelet XM_006516487.3 76.2% 82% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2418
ORF length:
2352
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag
61ttggcatgga cgcggacgac tcccgggccc ccaagggctc cttgcggaag ttcctggagc
121acctctccgg ggccggcaag gccatcggcg tgctgaccag cggcggggat gctcaaggta
181tgaacgctgc cgtccgtgcc gtggtgcgca tgggtatcta cgtgggggcc aaggtgtact
241tcatctacga gggctaccag ggcatggtgg acggaggctc aaacatcgca gaggccgact
301gggagagtgt ctccagcatc ctgcaagtgg gcgggacgat cattggcagt gcgcggtgcc
361aggccttccg cacgcgggaa ggccgcctga aggctgcttg caacctgctg cagcgcggca
421tcaccaacct gtgtgtgatc ggcggggacg ggagcctcac cggggccaac ctcttccgga
481aggagtggag tgggctgctg gaggagctgg ccaggaacgg ccagatcgat aaggaggccg
541tgcagaaata cgcctacctc aacgtggtgg gcatggtggg ctccatcgac aatgatttct
601gcggcaccga catgaccatc ggcacggact ccgccctgca caggatcatc gaggtcgtcg
661acgccatcat gaccacggcc cagagccacc agaggacctt cgttctggag gtgatgggac
721gacactgtgg gtacctggcc ctggtgagtg ccttggcctg cggtgcggac tgggtgttcc
781ttccagaatc tccaccagag gaaggctggg aggagcagat gtgtgtcaaa ctctcggaga
841accgtgcccg gaaaaaaagg ctgaatatta ttattgtggc tgaaggagca attgataccc
901aaaataaacc catcacctct gagaaaatca aagagcttgt cgtcacgcag ctgggctatg
961acacacgtgt gaccatcctc gggcacgtgc agagaggagg gaccccttcg gcattcgaca
1021ggatcttggc cagccgcatg ggagtggagg cagtcatcgc cttgctagag gccaccccgg
1081acaccccagc ttgcgtcgtg tcactgaacg ggaaccacgc cgtgcgcctg ccgctgatgg
1141agtgcgtgca gatgactcag gatgtgcaga aggcgatgga cgagaggaga tttcaagatg
1201cggttcgact ccgagggagg agctttgcgg gcaacctgaa cacctacaag cgacttgcca
1261tcaagctgcc ggatgatcag atcccaaaga ccaattgcaa cgtagctgtc atcaacgtgg
1321gggcacccgc ggctgggatg aacgcggccg tacgctcagc tgtgcgcgtg ggcattgccg
1381acggccacag gatgctcgcc atctatgatg gctttgacgg cttcgccaag ggccagatca
1441aagaaatcgg ctggacagat gtcgggggct ggaccggcca aggaggctcc attcttggga
1501caaaacgcgt tctcccgggg aagtacttgg aagagatcgc cacacagatg cgcacgcaca
1561gcatcaacgc gctgctgatc atcggtggat tcgaggccta cctgggactc ctggagctgt
1621cagccgcccg ggagaagcac gaggagttct gtgtccccat ggtcatggtt cccgctactg
1681tgtccaacaa tgtgccgggt tccgatttca gcatcggggc agacaccgcc ctgaacacta
1741tcaccgacac ctgcgaccgc atcaagcagt ccgccagcgg aaccaagcgg cgcgtgttca
1801tcatcgagac catgggcggc tactgtggct acctggccaa catggggggg ctcgcggctg
1861gagctgatgc cgcatacatt ttcgaagagc ccttcgacat cagggatctg cagtccaacg
1921tggagcacct gacggagaaa atgaagacca ccatccagag aggccttgtg ctcagaaatg
1981agagctgcag tgaaaactac accaccgact tcatttacca gctgtattca gaagagggca
2041aaggcgtgtt tgactgcagg aagaacgtgc tgggtcacat gcagcagggt ggggcaccct
2101ctccatttga tagaaacttt ggaaccaaaa tctctgccag agctatggag tggatcactg
2161caaaactcaa ggaggcccgg ggcagaggaa aaaaatttac caccgatgat tccatttgtg
2221tgctgggaat aagcaaaaga aacgttattt ttcaacctgt ggcagagctg aagaagcaaa
2281cggattttga gcacaggatt cccaaagaac agtggtggct caagctacgg cccctcatga
2341aaatcctggc caagtacaag gccagctatg acgtgtcgga ctcaggccag ctggaacatg
2401tgcagccctg gagtgtctnn ccaactttct tgtacaaagt tggcattata agaaagcatt
2461gcttatcaat ttgttgcaac gaac

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