Transcript: Mouse NM_001291071.1

Mus musculus phosphofructokinase, platelet (Pfkp), transcript variant 2, mRNA.

Source:
NCBI, updated 2017-06-04
Taxon:
Mus musculus (mouse)
Gene:
Pfkp (56421)
Length:
4033
CDS:
93..2447

Additional Resources:

NCBI RefSeq record:
NM_001291071.1
NBCI Gene record:
Pfkp (56421)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001291071.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000274765 TCGGAATGGTGATATCGATAA pLKO_005 536 CDS 100% 10.800 15.120 N Pfkp n/a
2 TRCN0000025948 GCAATCTATGACGGCTTTGAA pLKO.1 1422 CDS 100% 5.625 7.875 N Pfkp n/a
3 TRCN0000274764 TGACGGAGTCAGCAGTAATAA pLKO_005 2552 3UTR 100% 15.000 12.000 N Pfkp n/a
4 TRCN0000025894 GCAGAAGTATTCCTACCTCAA pLKO.1 566 CDS 100% 4.050 3.240 N Pfkp n/a
5 TRCN0000274698 ACGAAAGCTGCAGTGTAAATT pLKO_005 2002 CDS 100% 15.000 10.500 N Pfkp n/a
6 TRCN0000025916 GCCATTTGATATCGGAGATTT pLKO.1 1913 CDS 100% 13.200 9.240 N Pfkp n/a
7 TRCN0000274701 GCCATTTGATATCGGAGATTT pLKO_005 1913 CDS 100% 13.200 9.240 N Pfkp n/a
8 TRCN0000025919 GCTCTGCTAAACTCTGCATTT pLKO.1 3420 3UTR 100% 10.800 7.560 N Pfkp n/a
9 TRCN0000025962 GCGAGCTATGACATGTCTGAT pLKO.1 2385 CDS 100% 4.950 3.465 N Pfkp n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001291071.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06717 pDONR223 100% 80.1% 85.8% None (many diffs) n/a
2 ccsbBroad304_06717 pLX_304 0% 80.1% 85.8% V5 (many diffs) n/a
3 TRCN0000467766 TGCTGACACAGTGGCGCTGTTTGC pLX_317 19.2% 80.1% 85.8% V5 (many diffs) n/a
4 TRCN0000488232 TGCTCTAAATTATAAATGATCATC pLX_317 13% 80.1% 85.8% V5 (not translated due to prior stop codon) (many diffs) n/a
5 ccsbBroadEn_15527 pDONR223 0% 80.1% 85.8% None (many diffs) n/a
6 ccsbBroad304_15527 pLX_304 0% 80.1% 85.8% V5 (many diffs) n/a
7 ccsbBroadEn_14747 pDONR223 0% 80.1% 85.8% None (many diffs) n/a
8 ccsbBroad304_14747 pLX_304 0% 80.1% 85.8% V5 (many diffs) n/a
9 TRCN0000473407 TGCATTCACACTTTGGTCATCTTG pLX_317 19.4% 80.1% 85.8% V5 (many diffs) n/a
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