Construct: ORF ccsbBroadEn_15539

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001605.1_s300c1, BRDN0000395192
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PKN2 (5586)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5586 PKN2 protein kinase N2 NM_001320707.1 99.8% 99.8% 421T>C;1533T>C;2239T>C
2 human 5586 PKN2 protein kinase N2 XM_017001783.2 98.1% 98.1% (many diffs)
3 human 5586 PKN2 protein kinase N2 NM_006256.4 95% 95% (many diffs)
4 human 5586 PKN2 protein kinase N2 NM_001320709.2 93.3% 93.3% (many diffs)
5 human 5586 PKN2 protein kinase N2 XM_017001782.2 88.7% 88.6% (many diffs)
6 human 5586 PKN2 protein kinase N2 NM_001320708.2 79% 79% (many diffs)
7 human 5586 PKN2 protein kinase N2 XM_011541772.2 61.8% 61.5% (many diffs)
8 mouse 109333 Pkn2 protein kinase N2 NM_178654.4 85.5% 89.7% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2877
ORF length:
2808
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag
61ttggcaccat ggcgtccaac cccgaacggg gggagattct gctcacggaa ctgcaggggg
121attcccgaag tcttccgttt tctgagaatg tgagtgctgt tcaaaaatta gacttttcag
181atacaatggt gcagcagaaa ttggatgata tcaaggatcg aattaagaga gaaataagga
241aagaactgaa aatcaaagaa ggagctgaaa atctgaggaa agtcacaaca gataaaaaaa
301gtttggctta tgtagacaac attttgaaaa aatcaaataa aaaattagaa gaactacatc
361acaagctgca ggaattaaat gcacatattg ttgtatcaga tccagaagat attacagatt
421gcccaaggac tccagatact ccaaataatg accctcgttg ttctactagc aacaatagat
481tgaaggccct acaaaaacaa ttggatatag aacttaaagt aaaacaaggt gcagagaata
541tgatacagat gtattcaaat ggatcttcaa aggatcggaa actccatggt acagctcagc
601aactgctcca ggacagcaag acaaaaatag aagtcatacg aatgcagatt cttcaggcag
661tccagactaa tgaattggct tttgataatg caaaacctgt gataagtcct cttgaacttc
721ggatggaaga attaaggcat cattttagga tagagtttgc agtagcagaa ggtgcaaaga
781atgtaatgaa attacttggc tcaggaaaag taacagacag aaaagcactt tcagaagctc
841aagcaagatt taatgaatca agtcagaagt tggacctttt aaagtattca ttagagcaaa
901gattaaacga agtccccaag aatcatccca aaagcaggat tattattgaa gaactttcac
961ttgttgctgc atcaccaaca ctaagtccac gtcaaagtat gatatctacg caaaatcaat
1021atagtacact atccaaacca gcagcactaa caggtacttt ggaagttcgt cttatgggct
1081gccaagatat cctagagaat gtccctggac ggtcaaaagc aacatcagtt gcactgcctg
1141gttggagtcc aagtgaaacc agatcatctt tcatgagcag aacgagtaaa agtaaaagcg
1201gaagtagtcg aaatcttcta aaaaccgatg acttgtccaa tgatgtctgt gctgttttga
1261agctcgataa tactgtggtt ggccaaacta gctggaaacc catttccaat cagtcatggg
1321accagaagtt tacactggaa ctggacaggg ttaccttttt taatccagtt attgaaagaa
1381gaccaaaact tcaaagacaa aagaaaattt tttcaaagca acaaggcaaa acatttctca
1441gagctcctca aatgaatatt aatattgcca cttggggaag gctagtaaga agagctattc
1501ctacagtaaa tcattctggc accttcagcc ctcaagctcc tgtgcctact acagtgccag
1561tggttgatgt acgcatccct caactagcac ctccagctag cgattctaca gtaaccaaat
1621tggactttga tcttgagcct gaacctcctc cagccccacc acgagcttct tctcttggag
1681aaatagatga atcttctgaa ttaagagttt tggatatacc aggacaggat tcagagactg
1741tttttgatat tcagaatgac agaaatagta tacttccaaa atctcaatct gaatacaagc
1801ctgatactcc tcagtcaggc ctagaatata gtggtattca agaacttgag gacagaagat
1861ctcagcaaag gtttcagttt aatctacaag atttcaggtg ttgtgctgtc ttgggaagag
1921gacattttgg aaaggtgctt ttagctgaat ataaaaacac aaatgagatg tttgctataa
1981aagccttaaa gaaaggagat attgtggctc gagatgaagt agacagcctg atgtgtgaaa
2041aaagaatttt tgaaactgtg aatagtgtaa ggcatccctt tttggtgaac ctttttgcat
2101gtttccaaac caaagagcat gtttgctttg taatggaata tgctgccggt ggggacctaa
2161tgatgcacat tcatactgat gtcttttctg aaccaagagc tgtattttat gctgcttgtg
2221tagttcttgg gttgcagtat ttacatgaac acaaaattgt ttatagagat ttgaaattgg
2281ataacttatt gctagataca gagggccttg tgaaaattgc tgattttggt ctttgcaaag
2341aaggaatggg atatggagat agaacaagca cattttgtgg cactcctgaa tttcttgccc
2401cagaagtatt aacagaaact tcttatacaa gggctgtaga ttggtggggc cttggcgtgc
2461ttatatatga aatgcttgtt ggtgagtctc cctttcctgg tgatgatgaa gaggaagttt
2521ttgacagtat tgtaaatgat gaagtaaggt atccaaggtt cttatctaca gaagccattt
2581ctataatgag aaggctgtta agaagaaatc ctgaacggcg ccttggggct agcgagaaag
2641atgcagagga tgtaaaaaag cacccatttt tccggctaat tgattggagc gctctgatgg
2701acaaaaaagt aaagccacca tttataccta ccataagagg acgagaagat gttagtaatt
2761ttgatgatga atttacctca gaagcaccta ttctgactcc acctcgagaa ccaaggatac
2821tttcggaaga ggagcaggaa atgttcagag attttgacta cattgctgat tggtgttnnc
2881caactttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg
2941aac

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