Transcript: Human NM_001320707.1

Homo sapiens protein kinase N2 (PKN2), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-07-06
Taxon:
Homo sapiens (human)
Gene:
PKN2 (5586)
Length:
6055
CDS:
438..3248

Additional Resources:

NCBI RefSeq record:
NM_001320707.1
NBCI Gene record:
PKN2 (5586)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001147750 GGGATGCGTACATCAACCAC pXPR_003 TGG 1494 53% 10 0.8763 PKN2 PKN2 77926
2 BRDN0001487065 TTTGAAGCTCGATAATACTG pXPR_003 TGG 1204 43% 8 0.2344 PKN2 PKN2 77928
3 BRDN0001149537 TTGTTGCTAGTAGAACAACG pXPR_003 AGG 390 14% 3 -0.0434 PKN2 PKN2 77927
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001320707.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000380770 TGATATCAAGGATCGAATTAA pLKO_005 575 CDS 100% 15.000 21.000 N PKN2 n/a
2 TRCN0000194752 CAATCATGTAACAGGCTTATA pLKO.1 3869 3UTR 100% 13.200 18.480 N PKN2 n/a
3 TRCN0000353006 CAATCATGTAACAGGCTTATA pLKO_005 3869 3UTR 100% 13.200 18.480 N PKN2 n/a
4 TRCN0000379782 GGCCTAGAATATAGTGGTATT pLKO_005 2187 CDS 100% 10.800 15.120 N PKN2 n/a
5 TRCN0000377251 TTACCTCAGAAGCACCTATTC pLKO_005 3142 CDS 100% 10.800 15.120 N PKN2 n/a
6 TRCN0000195219 CTTAGCATGTTAGGGTCATTA pLKO.1 4704 3UTR 100% 13.200 10.560 N PKN2 n/a
7 TRCN0000219649 GTCCACGTCAAAGTATGATAT pLKO.1 1354 CDS 100% 13.200 10.560 N PKN2 n/a
8 TRCN0000332969 GTCCACGTCAAAGTATGATAT pLKO_005 1354 CDS 100% 13.200 10.560 N PKN2 n/a
9 TRCN0000422087 ATTGTACTTCAGCGTAAATAT pLKO_005 3428 3UTR 100% 15.000 10.500 N PKN2 n/a
10 TRCN0000006254 GCAGCAGAAATTGGATGATAT pLKO.1 560 CDS 100% 13.200 9.240 N PKN2 n/a
11 TRCN0000006251 GCAGGAATTAAATGCACATAT pLKO.1 737 CDS 100% 13.200 9.240 N PKN2 n/a
12 TRCN0000196735 GCAGGGTCTATTTACACAATT pLKO.1 5302 3UTR 100% 13.200 9.240 N PKN2 n/a
13 TRCN0000219650 TACTTTGGAAGTTCGTCTTAT pLKO.1 1424 CDS 100% 13.200 9.240 N PKN2 n/a
14 TRCN0000332970 TACTTTGGAAGTTCGTCTTAT pLKO_005 1424 CDS 100% 13.200 9.240 N PKN2 n/a
15 TRCN0000012716 CCAAGGTTCTTATCTACAGAA pLKO.1 2922 CDS 100% 4.950 3.465 N Pkn2 n/a
16 TRCN0000006252 CCACCATTTATACCTACCATA pLKO.1 3084 CDS 100% 4.950 3.465 N PKN2 n/a
17 TRCN0000006253 GCACATTCATACTGATGTCTT pLKO.1 2534 CDS 100% 4.950 3.465 N PKN2 n/a
18 TRCN0000006250 GCCACCAATAGCTTCTGAGTT pLKO.1 3325 3UTR 100% 4.950 3.465 N PKN2 n/a
19 TRCN0000084683 CCCAAGATCCTTTGGGAGAAA pLKO.1 4974 3UTR 100% 0.495 0.347 N Zfp646 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001320707.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15539 pDONR223 0% 99.8% 99.8% None 421T>C;1533T>C;2239T>C n/a
2 ccsbBroad304_15539 pLX_304 0% 99.8% 99.8% V5 421T>C;1533T>C;2239T>C n/a
3 TRCN0000489928 AAGTCCCACCATTGCGCAATTGCA pLX_317 14.6% 95.1% 95.1% V5 (not translated due to prior stop codon) 1280_1281ins144 n/a
4 ccsbBroadEn_15538 pDONR223 0% 6.4% 6.4% None 1_2628del n/a
5 ccsbBroad304_15538 pLX_304 0% 6.4% 6.4% V5 1_2628del n/a
6 TRCN0000468463 GCACGTAACCATTAAAGTGCCTCT pLX_317 100% 6.4% 6.4% V5 1_2628del n/a
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