Construct: ORF ccsbBroadEn_15726
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008999.1_s300c1, BRDN0000397360
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GRAP (10750)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10750 | GRAP | GRB2 related adaptor protein | NM_006613.4 | 100% | 100% | |
| 2 | human | 10750 | GRAP | GRB2 related adaptor protein | NM_001330148.2 | 61.4% | 47.4% | 299_300ins169;483_615del |
| 3 | human | 400581 | GRAPL | GRB2 related adaptor protei... | XM_017024635.2 | 59.4% | 49.7% | (many diffs) |
| 4 | human | 400581 | GRAPL | GRB2 related adaptor protei... | XM_017024636.2 | 59.4% | 49.7% | (many diffs) |
| 5 | human | 400581 | GRAPL | GRB2 related adaptor protei... | NM_001129778.3 | 48.5% | 47% | (many diffs) |
| 6 | human | 400581 | GRAPL | GRB2 related adaptor protei... | XM_017024637.1 | 48.5% | 47% | (many diffs) |
| 7 | human | 400581 | GRAPL | GRB2 related adaptor protei... | NM_001353418.2 | 48.2% | 43.2% | (many diffs) |
| 8 | human | 400581 | GRAPL | GRB2 related adaptor protei... | XM_011523857.3 | 46.8% | 45.6% | (many diffs) |
| 9 | mouse | 71520 | Grap | GRB2-related adaptor protein | NM_027817.3 | 87.4% | 92.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 717
- ORF length:
- 651
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag 61 ttggcatgga gtccgtggcc ctgtacagct ttcaggctac agagagcgac gagctggcct 121 tcaacaaggg agacacactc aagatcctga acatggagga tgaccagaac tggtacaagg 181 ccgagctccg gggtgtcgag ggatttattc ccaagaacta catccgcgtc aagccccatc 241 cgtggtactc gggcaggatt tcccggcagc tggccgaaga gattctgatg aagcggaacc 301 atctgggagc cttcctgatc cgggagagtg agagctcccc aggggagttc tctgtgtctg 361 tgaactatgg agaccaggtg cagcacttca aggtgctgcg tgaggcctcg gggaagtact 421 tcctgtggga ggagaagttc aactccctca acgagctggt cgacttctac cgcaccacca 481 ccatcgccaa gaagcggcag atcttcctgc gcgacgagga gcccttgctc aagtcacctg 541 gggcctgctt tgcccaggcc cagtttgact tctcagccca ggacccctcg cagctcagct 601 tccgccgtgg cgacatcatt gaggtcctgg agcgcccaga cccccactgg tggcggggcc 661 ggtcctgcgg gcgcgttggc ttcttcccac ggagttacgt gcagcccgtg cacctgtnnc 721 caactttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg 781 aac