Construct: ORF ccsbBroadEn_15819

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007046.1_s300c1, BRDN0000397561
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MEMO1 (51072)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 51072 MEMO1 mediator of cell motility 1 NM_001301833.3 100% 100%
2 human 51072 MEMO1 mediator of cell motility 1 NM_001371914.1 100% 100%
3 human 51072 MEMO1 mediator of cell motility 1 NM_001371915.1 100% 100%
4 human 51072 MEMO1 mediator of cell motility 1 NM_015955.4 100% 100%
5 human 51072 MEMO1 mediator of cell motility 1 NM_001371913.1 97% 97% 1_27del
6 human 51072 MEMO1 mediator of cell motility 1 NM_001137602.3 92.2% 92.2% 142_143ins69
7 human 51072 MEMO1 mediator of cell motility 1 NM_001371916.1 92.2% 92.2% 142_143ins69
8 human 51072 MEMO1 mediator of cell motility 1 NM_001371912.1 88.1% 88.1% 59_178del
9 human 51072 MEMO1 mediator of cell motility 1 NM_001301852.3 67.6% 34% 0_1ins145;291_292ins143
10 human 51072 MEMO1 mediator of cell motility 1 NM_001371917.1 67.6% 34% 0_1ins145;291_292ins143
11 human 51072 MEMO1 mediator of cell motility 1 NM_001371918.1 67.6% 34% 0_1ins145;291_292ins143
12 human 51072 MEMO1 mediator of cell motility 1 XM_011532896.2 67.6% 34% 0_1ins145;291_292ins143
13 human 51072 MEMO1 mediator of cell motility 1 NM_001371920.1 67% 67% 143_144ins294
14 human 51072 MEMO1 mediator of cell motility 1 NM_001371921.1 63.6% 37.8% 0_1ins181;255_256ins143
15 human 51072 MEMO1 mediator of cell motility 1 NR_163998.1 10.1% 1_84del;663_664ins77;899_7959del
16 human 51072 MEMO1 mediator of cell motility 1 NR_163997.1 9.6% 1_447del;1026_1027ins77;1262_8322del
17 human 51072 MEMO1 mediator of cell motility 1 NR_163996.1 8.3% 1_84del;520_521ins220;756_7816del
18 human 51072 MEMO1 mediator of cell motility 1 NR_163995.1 8.3% (many diffs)
19 human 51072 MEMO1 mediator of cell motility 1 NR_126032.2 8% 1_417del;853_854ins220;1089_8149del
20 human 51072 MEMO1 mediator of cell motility 1 NR_126034.3 8% (many diffs)
21 mouse 76890 Memo1 mediator of cell motility 1 NM_133771.2 96% 99.3% (many diffs)
22 mouse 76890 Memo1 mediator of cell motility 1 XM_006525109.3 96% 99.3% (many diffs)
23 mouse 76890 Memo1 mediator of cell motility 1 XM_011246712.2 64.9% 35.4% (many diffs)
24 mouse 76890 Memo1 mediator of cell motility 1 XM_011246713.2 64.9% 35.4% (many diffs)
25 mouse 76890 Memo1 mediator of cell motility 1 XR_001782128.1 27.3% (many diffs)
26 mouse 76890 Memo1 mediator of cell motility 1 XR_876585.2 25.5% (many diffs)
27 mouse 76890 Memo1 mediator of cell motility 1 XR_385390.3 24.6% (many diffs)
28 mouse 76890 Memo1 mediator of cell motility 1 XR_385387.3 24.4% (many diffs)
29 mouse 76890 Memo1 mediator of cell motility 1 XR_876587.2 18.8% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
957
ORF length:
891
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag
61ttggcatgtc caaccgagtg gtctgccgag aagccagtca cgccgggagc tggtacacag
121cctcaggacc gcagctgaat gcacagctag aaggttggct ttcacaagta cagtctacaa
181aaagacctgc tagagccatt attgcccccc atgcaggata tacgtactgt gggtcttgtg
241ctgcccatgc ttataaacaa gtggatccgt ctattacccg gagaattttc atccttgggc
301cttctcatca tgtgcccctc tctcgatgtg cactttccag tgtggatata tataggacac
361ctctgtatga ccttcgtatt gaccaaaaga tttacggaga actgtggaag acaggaatgt
421ttgaacgcat gtctctgcag acagatgaag atgaacacag tattgaaatg catttgcctt
481atacagctaa agccatggaa agccataagg atgagtttac cattattcct gtactggttg
541gagctctgag tgagtcaaaa gaacaggaat tcggaaaact cttcagtaaa tatctagcgg
601atcctagtaa tctctttgtg gtttcttctg atttctgcca ttggggtcaa aggttccgtt
661acagttacta tgatgaatcc cagggggaga tttatagatc cattgaacat ctagataaaa
721tgggtatgag tattatagaa caattagacc ctgtatcttt tagcaattac ttgaagaaat
781accataatac tatatgtgga agacatccca ttggggtgtt attaaatgct atcacagagc
841tccagaagaa tggaatgaat atgagttttt cgtttttgaa ttatgcccag tcgagccagt
901gtagaaactg gcaagacagt tcagtgagtt atgcagctgg agcactcacg gtccactnnc
961caactttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg
1021aac

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