Transcript: Mouse NM_133771.2

Mus musculus mediator of cell motility 1 (Memo1), mRNA.

Source:
NCBI, updated 2017-05-27
Taxon:
Mus musculus (mouse)
Gene:
Memo1 (76890)
Length:
1499
CDS:
112..1005

Additional Resources:

NCBI RefSeq record:
NM_133771.2
NBCI Gene record:
Memo1 (76890)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_133771.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000258053 TATCTAGCGGATCCTAGTAAT pLKO_005 637 CDS 100% 13.200 18.480 N Memo1 n/a
2 TRCN0000130305 GTCAAAGGTTCCGTTACAGTT pLKO.1 692 CDS 100% 4.950 6.930 N MEMO1 n/a
3 TRCN0000344179 GTCAAAGGTTCCGTTACAGTT pLKO_005 692 CDS 100% 4.950 6.930 N MEMO1 n/a
4 TRCN0000265278 ACTCTCCAGTGTGGATATATA pLKO_005 378 CDS 100% 15.000 10.500 N Memo1 n/a
5 TRCN0000250449 AGACCTGCTAGAGCCATTATT pLKO_005 229 CDS 100% 15.000 10.500 N Memo1 n/a
6 TRCN0000250451 CTCCTTAATTTCAACTCATTT pLKO_005 1155 3UTR 100% 13.200 9.240 N Memo1 n/a
7 TRCN0000215789 CATTTGCCTTATACAGCTAAA pLKO.1 517 CDS 100% 10.800 7.560 N Memo1 n/a
8 TRCN0000250450 CATTTGCCTTATACAGCTAAA pLKO_005 517 CDS 100% 10.800 7.560 N Memo1 n/a
9 TRCN0000201481 CCTAGCCTTTACCAAGATGTT pLKO.1 1062 3UTR 100% 4.950 3.465 N Memo1 n/a
10 TRCN0000122896 TGGAGCTCTGAGTGAGTCAAA pLKO.1 585 CDS 100% 4.950 3.465 N MEMO1 n/a
11 TRCN0000352982 TGGAGCTCTGAGTGAGTCAAA pLKO_005 585 CDS 100% 4.950 3.465 N MEMO1 n/a
12 TRCN0000201731 GCCTTTACCAAGATGTTGGTT pLKO.1 1066 3UTR 100% 0.300 0.210 N Memo1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_133771.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15819 pDONR223 0% 96% 99.3% None (many diffs) n/a
2 ccsbBroad304_15819 pLX_304 0% 96% 99.3% V5 (many diffs) n/a
3 TRCN0000470853 TAAACTCTGATTATTAATTGGTAT pLX_317 46.9% 96% 99.3% V5 (many diffs) n/a
4 ccsbBroadEn_08210 pDONR223 100% 95.9% 98.9% None (many diffs) n/a
5 ccsbBroad304_08210 pLX_304 0% 95.9% 98.9% V5 (many diffs) n/a
6 TRCN0000468812 CTTGATACTTGGCAAGCCATTGTG pLX_317 46.2% 95.9% 98.9% V5 (many diffs) n/a
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