Construct: ORF ccsbBroadEn_15942
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005666.1_s300c1, BRDN0000391101
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PELI1 (57162)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57162 | PELI1 | pellino E3 ubiquitin protei... | XM_011532997.2 | 52.3% | 52.3% | 1_342del |
| 2 | human | 57162 | PELI1 | pellino E3 ubiquitin protei... | XM_005264437.3 | 39% | 39% | 1_585del |
| 3 | human | 57162 | PELI1 | pellino E3 ubiquitin protei... | XM_011532996.2 | 39% | 39% | 1_585del |
| 4 | human | 57162 | PELI1 | pellino E3 ubiquitin protei... | XM_017004521.2 | 35.5% | 35.5% | 1_681del |
| 5 | human | 57162 | PELI1 | pellino E3 ubiquitin protei... | NM_020651.4 | 29.9% | 29.9% | 1_879del |
| 6 | human | 57162 | PELI1 | pellino E3 ubiquitin protei... | XM_011532994.3 | 29.9% | 29.9% | 1_879del |
| 7 | human | 57162 | PELI1 | pellino E3 ubiquitin protei... | XM_017004520.1 | 29.9% | 29.9% | 1_879del |
| 8 | mouse | 67245 | Peli1 | pellino 1 | XM_011243746.1 | 33.4% | 35.5% | (many diffs) |
| 9 | mouse | 67245 | Peli1 | pellino 1 | NM_023324.2 | 28.1% | 29.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 441
- ORF length:
- 375
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag 61 ttggcatgaa gaggaaagac gttgtagatg aaaaacaacc atgggtatat ctaaactgcg 121 gccatgtaca tggctatcat aactggggaa acaaagaaga acgtgatgga aaagatcgtg 181 aatgtcctat gtgtaggtct gttggtccct atgttcctct gtggcttgga tgtgaagctg 241 gattttatgt ggacgccggc cctccaaccc atgcgtttag cccgtgtggg catgtgtgtt 301 cagaaaagac aactgcctat tggtcccaga tcccacttcc tcatggtact catacttttc 361 atgcagcctg tcccttttgt gcacatcagt tggctggtga acaaggctac atcagactta 421 tttttcaagg acctctagac tnnccaactt tcttgtacaa agttggcatt ataagaaagc 481 attgcttatc aatttgttgc aacgaac