Transcript: Mouse NM_001081135.2

Mus musculus proline rich Gla (G-carboxyglutamic acid) 3 (transmembrane) (Prrg3), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-06-04
Taxon:
Mus musculus (mouse)
Gene:
Prrg3 (208748)
Length:
5947
CDS:
98..793

Additional Resources:

NCBI RefSeq record:
NM_001081135.2
NBCI Gene record:
Prrg3 (208748)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001081135.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000252397 AGAGCTCAGATGCTATGTATG pLKO_005 321 CDS 100% 10.800 15.120 N Prrg3 n/a
2 TRCN0000252401 ATGGCTGATGGAGCTAATATT pLKO_005 2469 3UTR 100% 15.000 10.500 N Prrg3 n/a
3 TRCN0000252399 GAAGAGGTCAAGGAAGTATTT pLKO_005 227 CDS 100% 13.200 9.240 N Prrg3 n/a
4 TRCN0000252398 ATGCCCATGCAGTCCTGAAAC pLKO_005 123 CDS 100% 10.800 7.560 N Prrg3 n/a
5 TRCN0000252400 TAGTCTTGCTGATTGTCATTG pLKO_005 363 CDS 100% 10.800 7.560 N Prrg3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001081135.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08914 pDONR223 100% 87.1% 93.9% None (many diffs) n/a
2 ccsbBroad304_08914 pLX_304 0% 87.1% 93.9% V5 (many diffs) n/a
3 TRCN0000480098 AACAACTCCGTTTAAACTAGGCGA pLX_317 50.5% 87.1% 93.9% V5 (many diffs) n/a
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