Transcript: Mouse NM_001081423.2

Mus musculus tubulin tyrosine ligase-like family, member 5 (Ttll5), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-04-17
Taxon:
Mus musculus (mouse)
Gene:
Ttll5 (320244)
Length:
4858
CDS:
236..4222

Additional Resources:

NCBI RefSeq record:
NM_001081423.2
NBCI Gene record:
Ttll5 (320244)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001081423.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000253179 AGTTGCCGAGTGGACTAATAA pLKO_005 2182 CDS 100% 15.000 21.000 N Ttll5 n/a
2 TRCN0000253181 CCGAGGACCTTGGATAGTAAA pLKO_005 754 CDS 100% 13.200 18.480 N Ttll5 n/a
3 TRCN0000253177 CGGACATCCAACCGGTCAATT pLKO_005 1448 CDS 100% 13.200 18.480 N Ttll5 n/a
4 TRCN0000253178 GCTGCTCACAGATGGTATAAA pLKO_005 4568 3UTR 100% 15.000 12.000 N Ttll5 n/a
5 TRCN0000253180 CCGAGCAGCACTGACTATAAT pLKO_005 506 CDS 100% 15.000 10.500 N Ttll5 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001081423.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11673 pDONR223 100% 17.9% 18.1% None (many diffs) n/a
2 ccsbBroad304_11673 pLX_304 0% 17.9% 18.1% V5 (many diffs) n/a
3 TRCN0000478925 ATCCGGCTGTATACCAGCTGTGGC pLX_317 57.9% 17.9% 18.1% V5 (many diffs) n/a
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