Transcript: Human NM_001082959.2

Homo sapiens scavenger receptor class B member 1 (SCARB1), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-09-27
Taxon:
Homo sapiens (human)
Gene:
SCARB1 (949)
Length:
3276
CDS:
145..1665

Additional Resources:

NCBI RefSeq record:
NM_001082959.2
NBCI Gene record:
SCARB1 (949)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001082959.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000056967 CTTCTATCTCTCCGTCTACTT pLKO.1 330 CDS 100% 4.950 3.465 N SCARB1 n/a
2 TRCN0000299709 CTTCTATCTCTCCGTCTACTT pLKO_005 330 CDS 100% 4.950 3.465 N SCARB1 n/a
3 TRCN0000056965 CGGATTATTTGCTGAGCTCAA pLKO.1 759 CDS 100% 4.050 2.835 N SCARB1 n/a
4 TRCN0000056966 GCAAGGTTGACTTCTGGCATT pLKO.1 866 CDS 100% 4.050 2.835 N SCARB1 n/a
5 TRCN0000299698 GCAAGGTTGACTTCTGGCATT pLKO_005 866 CDS 100% 4.050 2.835 N SCARB1 n/a
6 TRCN0000056964 GCTCATCATGACCTTGGCATT pLKO.1 612 CDS 100% 4.050 2.430 N SCARB1 n/a
7 TRCN0000310482 GCTCATCATGACCTTGGCATT pLKO_005 612 CDS 100% 4.050 2.430 N SCARB1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001082959.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10717 pDONR223 100% 90% 85.4% None (many diffs) n/a
2 ccsbBroad304_10717 pLX_304 0% 90% 85.4% V5 (many diffs) n/a
3 TRCN0000480951 AATTTGATCGGACCTGGTGAGCTG pLX_317 22.7% 90% 85.4% V5 (many diffs) n/a
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