Transcript: Human NM_001098844.3

Homo sapiens transmembrane protein 236 (TMEM236), mRNA.

Source:
NCBI, updated 2019-05-01
Taxon:
Homo sapiens (human)
Gene:
TMEM236 (653567)
Length:
5515
CDS:
96..1151

Additional Resources:

NCBI RefSeq record:
NM_001098844.3
NBCI Gene record:
TMEM236 (653567)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001098844.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000269489 CGTAGGTGTTTGCACTATAAA pLKO_005 1227 3UTR 100% 15.000 21.000 N TMEM236 n/a
2 TRCN0000269487 GGCTATACCCAGTCTACATAT pLKO_005 889 CDS 100% 13.200 18.480 N TMEM236 n/a
3 TRCN0000269439 TCGTGACTCTCTCTTACATTT pLKO_005 1072 CDS 100% 13.200 18.480 N TMEM236 n/a
4 TRCN0000269486 GAGCGTCCTGCTGCAAGATTT pLKO_005 971 CDS 100% 13.200 9.240 N TMEM236 n/a
5 TRCN0000269488 TTATTTCTCTCCTTCGAATTA pLKO_005 916 CDS 100% 13.200 9.240 N TMEM236 n/a
6 TRCN0000140820 GCTTGCCTTCCAGCTTTCTAA pLKO.1 3324 3UTR 100% 5.625 3.938 N TMEM236 n/a
7 TRCN0000121633 GAAGATTTACTCACAGTTGTT pLKO.1 3299 3UTR 100% 4.950 3.465 N TMEM236 n/a
8 TRCN0000142761 CATCTCCTGTTCTATTGCCTA pLKO.1 245 CDS 100% 2.640 1.848 N TMEM236 n/a
9 TRCN0000140467 GTGACTCTACTGATCTGGGTT pLKO.1 276 CDS 100% 2.640 1.848 N TMEM236 n/a
10 TRCN0000145465 GCCTTCATTCATCAGACATTT pLKO.1 3738 3UTR 100% 13.200 7.920 N TMEM236 n/a
11 TRCN0000122704 CCTACCAAGGAACAAGGGCTA pLKO.1 196 CDS 100% 2.160 1.296 N TMEM236 n/a
12 TRCN0000162795 CTTCCAAAGTGCTGGGATTAT pLKO.1 2195 3UTR 100% 13.200 6.600 Y SLC48A1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001098844.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05727 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_05727 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000479517 CTAACTTAACGATATTTTCATATA pLX_317 30.5% 100% 100% V5 n/a
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