Transcript: Human NM_001130858.2

Homo sapiens diphosphoinositol pentakisphosphate kinase 1 (PPIP5K1), transcript variant 5, mRNA.

Source:
NCBI, updated 2019-03-21
Taxon:
Homo sapiens (human)
Gene:
PPIP5K1 (9677)
Length:
5721
CDS:
205..4506

Additional Resources:

NCBI RefSeq record:
NM_001130858.2
NBCI Gene record:
PPIP5K1 (9677)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001148626 CGGTTGAGCACAGCATATCG pXPR_003 AGG 483 11% 6 0.7169 PPIP5K1, PPIP5K1P1-CATSPER2 PPIP5K1 76308
2 BRDN0001146565 GGAGTACGGGATCAGTCGGG pXPR_003 AGG 2338 54% 21 0.6746 PPIP5K1, PPIP5K1P1-CATSPER2 PPIP5K1 76306
3 BRDN0001146986 ACCTGCAGGTGTATACAGTG pXPR_003 GGG 786 18% 9 0.5906 PPIP5K1, PPIP5K1P1-CATSPER2 PPIP5K1 76307
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001130858.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000128325 GTAGATGGTAACTCCCAATTT pLKO.1 3760 CDS 100% 13.200 18.480 N PPIP5K1 n/a
2 TRCN0000149817 CAATCTGTTATCTCAGGGCAT pLKO.1 4404 CDS 100% 2.160 1.728 N PPIP5K1 n/a
3 TRCN0000146401 CTGGTCCATAAGTTCCATGTA pLKO.1 4249 CDS 100% 4.950 3.465 N PPIP5K1 n/a
4 TRCN0000128293 GAGATTGATAAACCATCCCAA pLKO.1 4429 CDS 100% 2.640 1.848 N PPIP5K1 n/a
5 TRCN0000128377 GCTACAAGACAGGGAAATTAA pLKO.1 1484 CDS 100% 15.000 7.500 Y PPIP5K1 n/a
6 TRCN0000431015 ACGTTCGAACGCGTCTCTATT pLKO_005 2699 CDS 100% 13.200 6.600 Y PPIP5K1 n/a
7 TRCN0000426823 AGCGTCTCTTTCGTAAGATTG pLKO_005 863 CDS 100% 10.800 5.400 Y PPIP5K1 n/a
8 TRCN0000428411 GGGCGCTATGATATCAGTAAG pLKO_005 2386 CDS 100% 10.800 5.400 Y PPIP5K1 n/a
9 TRCN0000178466 GCGCTATGATATCAGTAAGAT pLKO.1 2388 CDS 100% 5.625 2.813 Y Ppip5k1 n/a
10 TRCN0000198957 GATCAGGTATTTGCCCTGATT pLKO.1 2230 CDS 100% 0.495 0.248 Y Ppip5k1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001130858.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11402 pDONR223 100% 56.9% 57% None 1929G>A;2451_4299delinsG n/a
2 ccsbBroad304_11402 pLX_304 0% 56.9% 57% V5 1929G>A;2451_4299delinsG n/a
3 TRCN0000480975 CACTCCTTTAGCACTTATAGATCG pLX_317 16.6% 56.9% 57% V5 1929G>A;2451_4299delinsG n/a
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