Transcript: Human NM_001145839.1

Homo sapiens mitochondrial fission regulator 1 (MTFR1), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-08-04
Taxon:
Homo sapiens (human)
Gene:
MTFR1 (9650)
Length:
1472
CDS:
213..1259

Additional Resources:

NCBI RefSeq record:
NM_001145839.1
NBCI Gene record:
MTFR1 (9650)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001145839.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000143543 GCTTATCGGTATCGAAGTGAT pLKO.1 1059 CDS 100% 4.950 6.930 N MTFR1 n/a
2 TRCN0000343176 GCTTATCGGTATCGAAGTGAT pLKO_005 1059 CDS 100% 4.950 6.930 N MTFR1 n/a
3 TRCN0000140320 CAGAAGATTTGCGCTCTCGAA pLKO.1 636 CDS 100% 2.640 3.696 N MTFR1 n/a
4 TRCN0000139067 CGGTATCGAAGTGATAGCCAA pLKO.1 1065 CDS 100% 2.640 3.696 N MTFR1 n/a
5 TRCN0000144079 CAGTTTCAGATTAACAGCCAT pLKO.1 369 CDS 100% 2.640 2.112 N MTFR1 n/a
6 TRCN0000145051 CAAAGTACATCTGCTGTTGAT pLKO.1 819 CDS 100% 4.950 3.465 N MTFR1 n/a
7 TRCN0000122749 CCAAAGTACATCTGCTGTTGA pLKO.1 818 CDS 100% 4.950 3.465 N MTFR1 n/a
8 TRCN0000143523 GAGAGTGTTCAGCAAGACTAA pLKO.1 472 CDS 100% 4.950 3.465 N MTFR1 n/a
9 TRCN0000343121 GAGAGTGTTCAGCAAGACTAA pLKO_005 472 CDS 100% 4.950 3.465 N MTFR1 n/a
10 TRCN0000145407 GATAGCCAAGATGAAGTTGAA pLKO.1 1077 CDS 100% 4.950 2.970 N MTFR1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001145839.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02220 pDONR223 100% 91.4% 90.8% None (many diffs) n/a
2 ccsbBroad304_02220 pLX_304 0% 91.4% 90.8% V5 (many diffs) n/a
3 TRCN0000467790 GGGCTCTTACGGAATCGCGGTTCT pLX_317 35.2% 91.4% 90.8% V5 (many diffs) n/a
4 ccsbBroadEn_07457 pDONR223 100% 91.3% 90.5% None (many diffs) n/a
5 ccsbBroad304_07457 pLX_304 0% 91.3% 90.5% V5 (many diffs) n/a
6 TRCN0000472980 CAGTCCATCTTAATGCACCTGGCA pLX_317 37.4% 91.3% 90.5% V5 (many diffs) n/a
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