Transcript: Mouse NM_001160385.2

Mus musculus transmembrane protein 196 (Tmem196), transcript variant 2, mRNA.

Source:
NCBI, updated 2015-02-15
Taxon:
Mus musculus (mouse)
Gene:
Tmem196 (217951)
Length:
3707
CDS:
513..1043

Additional Resources:

NCBI RefSeq record:
NM_001160385.2
NBCI Gene record:
Tmem196 (217951)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001160385.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000341835 GAGCACCGCAACTGATGTTTA pLKO_005 997 CDS 100% 13.200 18.480 N Tmem196 n/a
2 TRCN0000341836 ATGACAGACAACATGAGTAAT pLKO_005 975 CDS 100% 13.200 9.240 N Tmem196 n/a
3 TRCN0000341832 CATTGGAGGCATCCTGAATTT pLKO_005 752 CDS 100% 13.200 9.240 N Tmem196 n/a
4 TRCN0000341833 CCTGCATCACTCTCATGAAAT pLKO_005 941 CDS 100% 13.200 9.240 N Tmem196 n/a
5 TRCN0000341834 CATCTCGCTTCCATGTCTTTG pLKO_005 822 CDS 100% 10.800 7.560 N Tmem196 n/a
6 TRCN0000142407 GAGGATGTTCTCAGAAAGGGA pLKO.1 914 CDS 100% 0.750 0.525 N TMEM196 n/a
7 TRCN0000122729 CTCTCATGAAATGGCTGAGAA pLKO.1 950 CDS 100% 0.495 0.347 N TMEM196 n/a
8 TRCN0000144487 CATGAAATGGCTGAGAAAGAA pLKO.1 954 CDS 100% 5.625 3.375 N TMEM196 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001160385.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13474 pDONR223 100% 44.4% 48% None (many diffs) n/a
2 ccsbBroad304_13474 pLX_304 0% 44.4% 48% V5 (many diffs) n/a
3 TRCN0000467242 TGTCGCACCGGAAGCGTACAAGAA pLX_317 100% 44.4% 48% V5 (many diffs) n/a
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