Construct: ORF TRCN0000467242
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003608.1_s317c1
- Derived from:
- ccsbBroadEn_13474
- DNA Barcode:
- TGTCGCACCGGAAGCGTACAAGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM196 (256130)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467242
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 256130 | TMEM196 | transmembrane protein 196 | NM_001366628.1 | 85.2% | 82.6% | (many diffs) |
| 2 | human | 256130 | TMEM196 | transmembrane protein 196 | NM_001366627.1 | 80.1% | 78.3% | 256_257delAG;270_333del |
| 3 | human | 256130 | TMEM196 | transmembrane protein 196 | NM_152774.3 | 51.5% | 50% | (many diffs) |
| 4 | human | 256130 | TMEM196 | transmembrane protein 196 | NM_001366626.1 | 49.8% | 48.3% | (many diffs) |
| 5 | human | 256130 | TMEM196 | transmembrane protein 196 | NM_001363562.2 | 49.7% | 48.6% | 1_204del;460_461delAG;474_537del |
| 6 | human | 256130 | TMEM196 | transmembrane protein 196 | XM_017011929.2 | 48.9% | 47.8% | 1_204del;460_461delAG;474_546del |
| 7 | human | 256130 | TMEM196 | transmembrane protein 196 | NM_001366625.1 | 48.1% | 47% | 1_222del;478_479delAG;492_555del |
| 8 | human | 256130 | TMEM196 | transmembrane protein 196 | XM_017011928.2 | 47.3% | 46.2% | 1_222del;478_479delAG;492_564del |
| 9 | mouse | 217951 | Tmem196 | transmembrane protein 196 | NM_001294154.1 | 44.6% | 48% | (many diffs) |
| 10 | mouse | 217951 | Tmem196 | transmembrane protein 196 | NM_001160385.2 | 44.4% | 48% | (many diffs) |
| 11 | mouse | 217951 | Tmem196 | transmembrane protein 196 | XM_006515787.3 | 43.2% | 46.4% | (many diffs) |
| 12 | mouse | 217951 | Tmem196 | transmembrane protein 196 | NM_001294146.1 | 42.9% | 46.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 333
- ORF length:
- 267
- Sequence:
-
1 tcttccattT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGAT CCTCTTTTCA GCCTGCTGTA TCTGTGGACT TATTGGGGGC ATCCTGAATT 121 TTCAGTTCCT CCGGGCAGTC ACAAAGAAAA CTTCCTCCCT ATACCCACTG CACCTTGCCT 181 CCATGTCTCT CGCGTGCATT GGGATCGGGG GCTGCACTCT CTCTTCCTGG CTCACTTGTC 241 GACTAGCCAG TTATGAACAG AGGAGGATGT TCTCAGAAAG GGAGCATTCC CTGCATCACT 301 CTCATGAAAT GGCTGAGAAA ATTGAGGGCT ATTACCCAAC TTTCTTGTAC AAAGTGGTTG 361 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 421 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATG 481 TCGCACCGGA AGCGTACAAG AAACGCGTTA AGTCgacaat caacctctgg attacaaaat 541 ttgtgaaaga tt