Transcript: Mouse NM_001165954.1

Mus musculus polyhomeotic-like 3 (Drosophila) (Phc3), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-05-27
Taxon:
Mus musculus (mouse)
Gene:
Phc3 (241915)
Length:
11051
CDS:
29..3010

Additional Resources:

NCBI RefSeq record:
NM_001165954.1
NBCI Gene record:
Phc3 (241915)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001165954.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000109012 CCAGTGGTTTATCAAGTAGAA pLKO.1 1805 CDS 100% 4.950 6.930 N Phc3 n/a
2 TRCN0000109014 CCTCATTCTCTAATCAAACAT pLKO.1 959 CDS 100% 5.625 4.500 N Phc3 n/a
3 TRCN0000434507 GTGAACATCGGTGACATTAAT pLKO_005 3191 3UTR 100% 15.000 10.500 N Phc3 n/a
4 TRCN0000422051 ATCAAGCATCCACCAGTTAAT pLKO_005 910 CDS 100% 13.200 9.240 N Phc3 n/a
5 TRCN0000143157 CAAGTCAGTCTCCTACTATAA pLKO.1 1272 CDS 100% 13.200 9.240 N PHC3 n/a
6 TRCN0000441135 CACAGACTGTTGCGGTAAATC pLKO_005 1752 CDS 100% 13.200 9.240 N Phc3 n/a
7 TRCN0000423511 CGACATGCTGTTCAGGTAATT pLKO_005 191 CDS 100% 13.200 9.240 N Phc3 n/a
8 TRCN0000415622 GGAATCGTAAGCCTGATAATC pLKO_005 2532 CDS 100% 13.200 9.240 N Phc3 n/a
9 TRCN0000109010 GCTACACTGAAAGACTTGTTA pLKO.1 3226 3UTR 100% 5.625 3.938 N Phc3 n/a
10 TRCN0000139438 CCAGTGGAAGACCATCTACAT pLKO.1 372 CDS 100% 4.950 3.465 N PHC3 n/a
11 TRCN0000139200 CCTTGCAGTCTATGCAGTCTT pLKO.1 1608 CDS 100% 4.950 3.465 N PHC3 n/a
12 TRCN0000109011 GCAGTGTTACACAACAGTCAA pLKO.1 402 CDS 100% 4.950 3.465 N Phc3 n/a
13 TRCN0000109013 GCTATTGTGAAACCACAGATT pLKO.1 2141 CDS 100% 4.950 3.465 N Phc3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001165954.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15997 pDONR223 0% 11.4% 11.9% None (many diffs) n/a
2 ccsbBroad304_15997 pLX_304 0% 11.4% 11.9% V5 (many diffs) n/a
3 TRCN0000473121 TTTTTCCGCTAGGGCAAAAGAACA pLX_317 96% 11.4% 11.9% V5 (many diffs) n/a
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