Transcript: Human NM_001178136.2

Homo sapiens pseudouridine 5'-phosphatase (PUDP), transcript variant 4, mRNA.

Source:
NCBI, updated 2019-08-09
Taxon:
Homo sapiens (human)
Gene:
PUDP (8226)
Length:
1974
CDS:
41..598

Additional Resources:

NCBI RefSeq record:
NM_001178136.2
NBCI Gene record:
PUDP (8226)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001178136.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000370326 CCCAGTCTAACGTGTTGATAA pLKO_005 748 3UTR 100% 13.200 18.480 N PUDP n/a
2 TRCN0000365199 GTAATCGCTATGACAAGAAAT pLKO_005 141 CDS 100% 13.200 18.480 N PUDP n/a
3 TRCN0000365256 TGGATACTGAACGGCTGTATT pLKO_005 99 CDS 100% 13.200 18.480 N PUDP n/a
4 TRCN0000051203 CGTCGTTCGATATGAAGACAA pLKO.1 264 CDS 100% 4.950 6.930 N PUDP n/a
5 TRCN0000051207 GACGGAAACTTGAGCCGAGAT pLKO.1 497 CDS 100% 4.050 5.670 N PUDP n/a
6 TRCN0000051204 CAGTGGTGTTTCAAGAAATAT pLKO.1 120 CDS 100% 15.000 10.500 N PUDP n/a
7 TRCN0000365198 GTGTATCAGTTTGGTAATATG pLKO_005 927 3UTR 100% 13.200 9.240 N PUDP n/a
8 TRCN0000370324 TCATCATCCACCTGCGGAAAC pLKO_005 204 CDS 100% 6.000 4.200 N PUDP n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001178136.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11262 pDONR223 100% 74.7% 74.5% None (many diffs) n/a
2 ccsbBroad304_11262 pLX_304 0% 74.7% 74.5% V5 (many diffs) n/a
3 TRCN0000472581 CCAGACCTTACAAATCCACTCGAC pLX_317 59% 74.7% 74.5% V5 (many diffs) n/a
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