Construct: ORF TRCN0000472581
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012700.1_s317c1
- Derived from:
- ccsbBroadEn_11262
- DNA Barcode:
- CCAGACCTTACAAATCCACTCGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PUDP (8226)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472581
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8226 | PUDP | pseudouridine 5'-phosphatase | NM_012080.5 | 93.5% | 93.4% | 1_42del;346G>A;456G>A |
| 2 | human | 8226 | PUDP | pseudouridine 5'-phosphatase | NM_001135565.2 | 84.9% | 84.8% | (many diffs) |
| 3 | human | 8226 | PUDP | pseudouridine 5'-phosphatase | NM_001178136.2 | 74.7% | 74.5% | (many diffs) |
| 4 | human | 8226 | PUDP | pseudouridine 5'-phosphatase | NM_001178135.2 | 74.2% | 66.8% | (many diffs) |
| 5 | human | 8226 | PUDP | pseudouridine 5'-phosphatase | XR_001755734.1 | 36.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 708
- ORF length:
- 642
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga cggacttctt ctggatactg aacggctgta ttcagtggtg tttcaagaaa 121 tatgtaatcg ctatgacaag aaatacagct gggatgtaaa gtccctggtt atgggtaaga 181 aggcattaga ggcggcacag attataatag acgtcttgca gctcccgatg tccaaagagg 241 agctggtgga agaaagccaa acgaagttaa aggaagtgtt ccccacggct gcgctcatgc 301 caggggcgga gaaactcatc atccaccTGC GGAAACATGG CATCCCCTTT GCACTGGCCA 361 CCAGCTCGAG GTCCGCGTCG TTCGATATGA AGACAAGCCG CCACAAGGAG TTCTTCAGCT 421 TGTTTTCCCA CATTGTGCTG GGAGATGACC CCGAAGTGCA GCATGGCAAG CCAGACCCAG 481 ACATCTTCCT AGCTTGTGCC AAGAGGTTCT CTCCCCCTCC TGCTATGGAG AAGTGCCTTG 541 TCTTTGAAGA TGCTCCCAAT GGGGTGGAGG CGGCCCTGGC AGCTGGGATG CAGGTGGTCA 601 TGGTTCCTGA CGGAAACTTG AGCCGAGATC TGACAACAAA GGCCACCCTG GTGCTGAATT 661 CCCTGCAGGA CTTCCAGCCC GAGCTGTTTG GTTTGCCCTC CTATGAGTGC CCAACTGTCT 721 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 781 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 841 GTGGAAAGGA CGACCAGACC TTACAAATCC ACTCGACACG CGTTAAGTCg acaatcaacc 901 tctggattac aaaatttgtg aaagatt