Transcript: Human NM_001204798.2

Homo sapiens potassium voltage-gated channel subfamily H member 2 (KCNH2), transcript variant 4, mRNA.

Source:
NCBI, updated 2019-08-27
Taxon:
Homo sapiens (human)
Gene:
KCNH2 (3757)
Length:
2438
CDS:
302..1948

Additional Resources:

NCBI RefSeq record:
NM_001204798.2
NBCI Gene record:
KCNH2 (3757)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001204798.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000068385 CCCTCCATCAAGGACAAGTAT pLKO.1 1094 CDS 100% 5.625 3.938 N Kcnh2 n/a
2 TRCN0000429152 CAGATAGGCAAACCCTACAAC pLKO_005 1055 CDS 100% 4.950 3.465 N KCNH2 n/a
3 TRCN0000021717 CCTCATGTATGCTAGCATCTT pLKO.1 1228 CDS 100% 4.950 3.465 N KCNH2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001204798.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14289 pDONR223 100% 35.4% 31.9% None (many diffs) n/a
2 ccsbBroad304_14289 pLX_304 0% 35.4% 31.9% V5 (not translated due to prior stop codon) (many diffs) n/a
3 TRCN0000468367 GATACGGCATTCACCCGCCGGGGG pLX_317 29% 35.4% 31.9% V5 (not translated due to prior stop codon) (many diffs) n/a
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