Construct: ORF TRCN0000468367

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003257.1_s317c1
Derived from:
ccsbBroadEn_14289
DNA Barcode:
GATACGGCATTCACCCGCCGGGGG
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
KCNH6 (81033)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000468367
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 81033 KCNH6 potassium voltage-gated cha... XM_011525313.1 70.4% 70.1% (many diffs)
2 human 81033 KCNH6 potassium voltage-gated cha... XM_011525311.1 68.9% 68.7% (many diffs)
3 human 81033 KCNH6 potassium voltage-gated cha... XM_011525312.1 68.9% 68.7% (many diffs)
4 human 81033 KCNH6 potassium voltage-gated cha... XR_934568.1 57.5% (many diffs)
5 human 81033 KCNH6 potassium voltage-gated cha... XM_011525309.2 54.7% 54.5% (many diffs)
6 human 81033 KCNH6 potassium voltage-gated cha... XM_011525308.2 54.5% 54.3% (many diffs)
7 human 81033 KCNH6 potassium voltage-gated cha... XM_017025178.2 52.8% 52.6% (many diffs)
8 human 81033 KCNH6 potassium voltage-gated cha... NM_001278919.2 52.2% 52% (many diffs)
9 human 81033 KCNH6 potassium voltage-gated cha... XM_017025177.1 52.2% 52% (many diffs)
10 human 81033 KCNH6 potassium voltage-gated cha... XM_017025176.2 50.9% 50.7% (many diffs)
11 human 81033 KCNH6 potassium voltage-gated cha... NM_030779.4 50.3% 50.2% (many diffs)
12 human 81033 KCNH6 potassium voltage-gated cha... XM_017025175.1 50.3% 50.2% (many diffs)
13 human 81033 KCNH6 potassium voltage-gated cha... NM_173092.3 46.7% 46.4% (many diffs)
14 human 81033 KCNH6 potassium voltage-gated cha... XM_011525310.2 46.7% 46.4% (many diffs)
15 human 81033 KCNH6 potassium voltage-gated cha... XM_017025179.2 45% 44.7% (many diffs)
16 human 81033 KCNH6 potassium voltage-gated cha... NM_001278920.2 39.4% 39.2% (many diffs)
17 human 81033 KCNH6 potassium voltage-gated cha... XM_017025180.2 37.9% 37.8% (many diffs)
18 human 3757 KCNH2 potassium voltage-gated cha... NM_001204798.2 35.4% 31.9% (many diffs)
19 human 3757 KCNH2 potassium voltage-gated cha... NM_172057.2 26% 23.3% (many diffs)
20 mouse 192775 Kcnh6 potassium voltage-gated cha... XM_006532558.3 73.5% 76.6% (many diffs)
21 mouse 192775 Kcnh6 potassium voltage-gated cha... XM_006532554.2 57.8% 61.8% (many diffs)
22 mouse 192775 Kcnh6 potassium voltage-gated cha... XM_006532553.1 57.7% 61.7% (many diffs)
23 mouse 192775 Kcnh6 potassium voltage-gated cha... XM_006532552.1 56.9% 60.8% (many diffs)
24 mouse 192775 Kcnh6 potassium voltage-gated cha... XM_006532550.2 50% 53.4% (many diffs)
25 mouse 192775 Kcnh6 potassium voltage-gated cha... XR_388374.2 48.8% (many diffs)
26 mouse 192775 Kcnh6 potassium voltage-gated cha... NM_001037712.1 47.1% 50.4% (many diffs)
27 mouse 192775 Kcnh6 potassium voltage-gated cha... XR_388373.2 46.7% (many diffs)
28 mouse 192775 Kcnh6 potassium voltage-gated cha... XM_006532551.3 35.2% 37.5% (many diffs)
29 mouse 192775 Kcnh6 potassium voltage-gated cha... XR_001779926.1 23.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1569
ORF length:
1503
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgcc ggtccgcagg ggccacgtcg ctccccaaaa cacttacctg gacaccatca
121tccgcaagtt cgagggccaa agtcggaagt tcctgattgc caatgctcag atggagaact
181gcgccatcat ttactgcaac gacggcttct gcgaactctt cggctactcc cgagtggagg
241tgatgcagca accctgcacc tgcgacttcc tcacaggccc caacacacca agcagcgccg
301tgtcccgcct agcgcaggcc ctgctggggg ctgaggagtg caaggtggac atcctctact
361accgcaagga tgcctccagc ttccgctgcc tggtagatgt ggtgcccgtg aagaacgagg
421acggggctgt catcatgttc attctcaact tcgaggacct ggcccagctc ctggccaagt
481gcagcagccg cagcttgtcc cagcgcctgt tgtcccagag cttcctgggc tccgagggct
541ctcatggcag gccaggcgga ccagggccag gcacaggcag gggcaagtac aggaccatca
601gccagatccc acagttcacg ctcaacttcg tggagttcaa cttggagaag caccgctcca
661gctccaccac ggagattgag atcatcgcgc cccataaggt ggtggagcgg acacagaacg
721tcactgagaa ggtcacccag gtcctgtccc tgggcgcgga tgtgctgccg gagtacaagc
781tgcaggcgcc gcgcatccac cgctggacca tcctgcacta cagccccttc aaggccgtgt
841gggactggct catcctgctg ctggtcatct acacggctgt cttcacgccc tactcagccg
901ccttcctgct cagcgaccag gacgaatcac ggcgtggggc ctgcagctat acctgcagtc
961ccctcactgt ggtggatctc atcgtggaca tcatgttcgt cgtggacatc gtcatcaact
1021tccgcaccac ctatgtcaac accaatgatg aggtggtcag ccacccccgc cgcatcgccg
1081tccactactt caagggctgg ttcctcattg acatggtggc cgccatccct ttcgacctcc
1141TGATCTTCCG CACTGGCTCC GATGAGACCA CAACCCTGAT TGGGCTATTG AAGACAGCGC
1201GGCTGCTGCG GCTGGTGCGC GTAGCACGGA AGCTGGACCG CTACTCTGAG TATGGGGCGG
1261CTGTGCTCTT CTTGCTCATG TGCACCTTCG CGCTCATAGC GCACTGGCTG GCCTGCATCT
1321GGTACGCCAT CGGCAATGTG GAGCGGCCCT ACCTAGAACA CAAGATCGGC TGGCTGGACA
1381GCCTGGGTGT GCAGCTTGGC AAGCGCTACA ACGGCAGCGA CCCAGCCTCG GGCCCCTCGG
1441TGCAGGACAA GTATGTCACA GCCCTCTACT TCACCTTCAG CAGCCTCACC AGCGTGGGCT
1501TCGGCAATGT CTCGCCCAAC ACCAACTCCG AGAAGGTCTT CTCCATCTGC GTCATGCTCA
1561TCCGGTTGTG AGTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT
1621AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT
1681TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGA TACGGCATTC ACCCGCCGGG
1741GGACGCGTTA AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt

Download FASTA (ORF) (Full)